Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917
The probiotic bacterium Escherichia coli Nissle 1917 (EcN) holds significant promise for use in clinical and biological industries. However, the reliance on antibiotics to maintain plasmid-borne genes has overshadowed its benefits. In this study, we addressed this issue by engineering the endogenous...
Main Authors: | Siyan Zhou, Linlin Zhao, Wenjie Zuo, Yilin Zheng, Ping Zhang, Yanan Sun, Yang Wang, Guocheng Du, Zhen Kang |
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Format: | Article |
Language: | English |
Published: |
KeAi Communications Co., Ltd.
2024-03-01
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Series: | Synthetic and Systems Biotechnology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X24000061 |
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