Effect of ginsenoside CK combined with cisplatin on the proliferation and migration of human cervical cancer HeLa cells via Ras/ERK/MAPK pathway
Ginsenoside (G-CK) has good chemo preventive and anticancer effects. However, its antimetastatic effects and underlying mechanisms on cervical cancer (CC) are still not clear. This study found that both G-CK and DDP inhibited the proliferation of HeLa cells in a concentration-dependent manner, and t...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623000385 |
| _version_ | 1811155513376768000 |
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| author | Xiao-Li Li Qi Yin Wei Wang Run-Hui Ma Zhi-Jing Ni Kiran Thakur Jian-Guo Zhang Zhao-Jun Wei |
| author_facet | Xiao-Li Li Qi Yin Wei Wang Run-Hui Ma Zhi-Jing Ni Kiran Thakur Jian-Guo Zhang Zhao-Jun Wei |
| author_sort | Xiao-Li Li |
| collection | DOAJ |
| description | Ginsenoside (G-CK) has good chemo preventive and anticancer effects. However, its antimetastatic effects and underlying mechanisms on cervical cancer (CC) are still not clear. This study found that both G-CK and DDP inhibited the proliferation of HeLa cells in a concentration-dependent manner, and the combination blocked cell growth and colony-forming ability, and inhibited cell migration and EMT processes. The signature molecule of EMT, E-cadherin, was up-regulated, and both N-cadherin and vimentin were down-regulated and cell migration was affected through inhibition of RAS/MEK/ERK and PI3K/AKT signaling pathways. DDP could induce oxidative stress damage in HEK293 cells, as evidenced by the accumulation of ROS, decrease in GSH levels and oxidative stress antioxidant enzymes (CAT, SOD and GSH-Px) and increase in GSSG and MDA levels, which proved that G-CK exerted inhibitory on HeLa cell proliferation and EMT in combination with traditional anti-cancer drug and protected normal cells from DDP-induced oxidative stress damage. |
| first_indexed | 2024-04-10T04:35:34Z |
| format | Article |
| id | doaj.art-cd645492ff9a4ccab0c50a5bbbf35888 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-10T04:35:34Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-cd645492ff9a4ccab0c50a5bbbf358882023-03-10T04:34:29ZengElsevierJournal of Functional Foods1756-46462023-03-01102105438Effect of ginsenoside CK combined with cisplatin on the proliferation and migration of human cervical cancer HeLa cells via Ras/ERK/MAPK pathwayXiao-Li Li0Qi Yin1Wei Wang2Run-Hui Ma3Zhi-Jing Ni4Kiran Thakur5Jian-Guo Zhang6Zhao-Jun Wei7School of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People’s Republic of ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People’s Republic of ChinaSchool of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People’s Republic of ChinaSchool of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People’s Republic of ChinaSchool of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People’s Republic of ChinaSchool of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People’s Republic of ChinaSchool of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People’s Republic of China; Corresponding authors at: School of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China.School of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People’s Republic of China; Corresponding authors at: School of Biological Science and Engineering, Yinchuan 750021, People’s Republic of China.Ginsenoside (G-CK) has good chemo preventive and anticancer effects. However, its antimetastatic effects and underlying mechanisms on cervical cancer (CC) are still not clear. This study found that both G-CK and DDP inhibited the proliferation of HeLa cells in a concentration-dependent manner, and the combination blocked cell growth and colony-forming ability, and inhibited cell migration and EMT processes. The signature molecule of EMT, E-cadherin, was up-regulated, and both N-cadherin and vimentin were down-regulated and cell migration was affected through inhibition of RAS/MEK/ERK and PI3K/AKT signaling pathways. DDP could induce oxidative stress damage in HEK293 cells, as evidenced by the accumulation of ROS, decrease in GSH levels and oxidative stress antioxidant enzymes (CAT, SOD and GSH-Px) and increase in GSSG and MDA levels, which proved that G-CK exerted inhibitory on HeLa cell proliferation and EMT in combination with traditional anti-cancer drug and protected normal cells from DDP-induced oxidative stress damage.http://www.sciencedirect.com/science/article/pii/S1756464623000385G-CKAntimetastaticEMTRAS/MEK/ERKPI3K/AKTOxidative stress |
| spellingShingle | Xiao-Li Li Qi Yin Wei Wang Run-Hui Ma Zhi-Jing Ni Kiran Thakur Jian-Guo Zhang Zhao-Jun Wei Effect of ginsenoside CK combined with cisplatin on the proliferation and migration of human cervical cancer HeLa cells via Ras/ERK/MAPK pathway Journal of Functional Foods G-CK Antimetastatic EMT RAS/MEK/ERK PI3K/AKT Oxidative stress |
| title | Effect of ginsenoside CK combined with cisplatin on the proliferation and migration of human cervical cancer HeLa cells via Ras/ERK/MAPK pathway |
| title_full | Effect of ginsenoside CK combined with cisplatin on the proliferation and migration of human cervical cancer HeLa cells via Ras/ERK/MAPK pathway |
| title_fullStr | Effect of ginsenoside CK combined with cisplatin on the proliferation and migration of human cervical cancer HeLa cells via Ras/ERK/MAPK pathway |
| title_full_unstemmed | Effect of ginsenoside CK combined with cisplatin on the proliferation and migration of human cervical cancer HeLa cells via Ras/ERK/MAPK pathway |
| title_short | Effect of ginsenoside CK combined with cisplatin on the proliferation and migration of human cervical cancer HeLa cells via Ras/ERK/MAPK pathway |
| title_sort | effect of ginsenoside ck combined with cisplatin on the proliferation and migration of human cervical cancer hela cells via ras erk mapk pathway |
| topic | G-CK Antimetastatic EMT RAS/MEK/ERK PI3K/AKT Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623000385 |
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