A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers
Polymeric nanocarriers must overcome several biological barriers to reach the vicinity of solid tumors and deliver their encapsulated drug. This study assessed the in vitro and in vivo passage through the blood vessel wall to tumors of two well-characterized polymeric nanocarriers: poly(ethyleneglyc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10717544.2018.1510064 |
| _version_ | 1818487412131627008 |
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| author | Agathe Figarol Laure Gibot Muriel Golzio Barbara Lonetti Anne-Françoise Mingotaud Marie-Pierre Rols |
| author_facet | Agathe Figarol Laure Gibot Muriel Golzio Barbara Lonetti Anne-Françoise Mingotaud Marie-Pierre Rols |
| author_sort | Agathe Figarol |
| collection | DOAJ |
| description | Polymeric nanocarriers must overcome several biological barriers to reach the vicinity of solid tumors and deliver their encapsulated drug. This study assessed the in vitro and in vivo passage through the blood vessel wall to tumors of two well-characterized polymeric nanocarriers: poly(ethyleneglycol-b-ε-caprolactone) micelles and polymersomes charged with a fluorescent membrane dye (DiO: 3,3'-dioctadecyloxacarbo-cyanine perchlorate). The internalization and translocation from endothelial (human primary endothelial cells HUVEC) to cancer cells (human tumor cell line HCT-116) was studied in conventional 2D monolayers, 3D tumor spheroids, or in an endothelium model based on transwell assay. Micelles induced a faster DiO internalization compared to polymersomes but the latter crossed the endothelial monolayer more easily. Both translocation rates were enhanced by the addition of a pro-inflammatory factor or in the presence of tumor cells. These results were confirmed by early in vivo experiments. Overall, this study pointed out the room for the improvement of polymeric nanocarriers design to avoid drug losses when crossing the blood vessel walls. |
| first_indexed | 2024-12-10T16:37:27Z |
| format | Article |
| id | doaj.art-cd68bda4ea2048ddbb4e589f3e0d7133 |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-10T16:37:27Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-cd68bda4ea2048ddbb4e589f3e0d71332022-12-22T01:41:21ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642018-01-012511766177810.1080/10717544.2018.15100641510064A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriersAgathe Figarol0Laure Gibot1Muriel Golzio2Barbara Lonetti3Anne-Françoise Mingotaud4Marie-Pierre Rols5Université de Toulouse, CNRS, UPSUniversité de Toulouse, CNRS, UPSUniversité de Toulouse, CNRS, UPSUniversité de Toulouse CNRS UMRUniversité de Toulouse CNRS UMRUniversité de Toulouse, CNRS, UPSPolymeric nanocarriers must overcome several biological barriers to reach the vicinity of solid tumors and deliver their encapsulated drug. This study assessed the in vitro and in vivo passage through the blood vessel wall to tumors of two well-characterized polymeric nanocarriers: poly(ethyleneglycol-b-ε-caprolactone) micelles and polymersomes charged with a fluorescent membrane dye (DiO: 3,3'-dioctadecyloxacarbo-cyanine perchlorate). The internalization and translocation from endothelial (human primary endothelial cells HUVEC) to cancer cells (human tumor cell line HCT-116) was studied in conventional 2D monolayers, 3D tumor spheroids, or in an endothelium model based on transwell assay. Micelles induced a faster DiO internalization compared to polymersomes but the latter crossed the endothelial monolayer more easily. Both translocation rates were enhanced by the addition of a pro-inflammatory factor or in the presence of tumor cells. These results were confirmed by early in vivo experiments. Overall, this study pointed out the room for the improvement of polymeric nanocarriers design to avoid drug losses when crossing the blood vessel walls.http://dx.doi.org/10.1080/10717544.2018.1510064nanocarrierspolymersself-assemblydrug deliverytranslocationendotheliumtumor |
| spellingShingle | Agathe Figarol Laure Gibot Muriel Golzio Barbara Lonetti Anne-Françoise Mingotaud Marie-Pierre Rols A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers Drug Delivery nanocarriers polymers self-assembly drug delivery translocation endothelium tumor |
| title | A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers |
| title_full | A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers |
| title_fullStr | A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers |
| title_full_unstemmed | A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers |
| title_short | A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers |
| title_sort | journey from the endothelium to the tumor tissue distinct behavior between peo pcl micelles and polymersomes nanocarriers |
| topic | nanocarriers polymers self-assembly drug delivery translocation endothelium tumor |
| url | http://dx.doi.org/10.1080/10717544.2018.1510064 |
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