Isolation and Characterization of the <i>Acadevirus</i> Members BigMira and MidiMira Infecting a Highly Pathogenic <i>Proteus mirabilis</i> Strain
<i>Proteus mirabilis</i> is an opportunistic pathogen and is responsible for more than 40% of all cases of catheter-associated urinary tract infections (CAUTIs). Healthcare-associated infections have been aggravated by the constant emergence of antibiotic-resistant bacterial strains. Bec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/9/2141 |
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| author | Jéssica Duarte da Silva Lene Bens Adriele J. do Carmo Santos Rob Lavigne José Soares Luís D. R. Melo Marta Vallino Roberto Sousa Dias Zuzanna Drulis-Kawa Sérgio Oliveira de Paula Jeroen Wagemans |
| author_facet | Jéssica Duarte da Silva Lene Bens Adriele J. do Carmo Santos Rob Lavigne José Soares Luís D. R. Melo Marta Vallino Roberto Sousa Dias Zuzanna Drulis-Kawa Sérgio Oliveira de Paula Jeroen Wagemans |
| author_sort | Jéssica Duarte da Silva |
| collection | DOAJ |
| description | <i>Proteus mirabilis</i> is an opportunistic pathogen and is responsible for more than 40% of all cases of catheter-associated urinary tract infections (CAUTIs). Healthcare-associated infections have been aggravated by the constant emergence of antibiotic-resistant bacterial strains. Because of this, the use of phages to combat bacterial infections gained renewed interest. In this study, we describe the biological and genomic features of two <i>P. mirabilis</i> phages, named BigMira and MidiMira. These phages belong to the <i>Acadevirus</i> genus (family <i>Autographiviridae</i>). BigMira and MidiMira are highly similar, differing only in four missense mutations in their phage tail fiber. These mutations are sufficient to impact the phages’ depolymerase activity. Subsequently, the comparative genomic analysis of ten clinical <i>P. mirabilis</i> strains revealed differences in their antibiotic resistance profiles and lipopolysaccharide locus, with the latter potentially explaining the host range data of the phages. The massive presence of antimicrobial resistance genes, especially in the phages’ isolation strain <i>P. mirabilis</i> MCS, highlights the challenges in treating infections caused by multidrug-resistant bacteria. The findings reinforce BigMira and MidiMira phages as candidates for phage therapy purposes. |
| first_indexed | 2024-03-10T22:27:26Z |
| format | Article |
| id | doaj.art-cd6aa22362544162bb22037cfd60469e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T22:27:26Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cd6aa22362544162bb22037cfd60469e2023-11-19T12:01:08ZengMDPI AGMicroorganisms2076-26072023-08-01119214110.3390/microorganisms11092141Isolation and Characterization of the <i>Acadevirus</i> Members BigMira and MidiMira Infecting a Highly Pathogenic <i>Proteus mirabilis</i> StrainJéssica Duarte da Silva0Lene Bens1Adriele J. do Carmo Santos2Rob Lavigne3José Soares4Luís D. R. Melo5Marta Vallino6Roberto Sousa Dias7Zuzanna Drulis-Kawa8Sérgio Oliveira de Paula9Jeroen Wagemans10Laboratory of Molecular Immunovirology, Department of Microbiology, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilLaboratory of Gene Technology, Department of Biosystems, Division of Animal and Human Health Engineering, KU Leuven, 3000 Leuven, BelgiumLaboratory of Molecular Immunovirology, Department of Microbiology, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilLaboratory of Gene Technology, Department of Biosystems, Division of Animal and Human Health Engineering, KU Leuven, 3000 Leuven, BelgiumLaboratory of Molecular Immunovirology, Department of Microbiology, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilCentre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalInstitute for Sustainable Plant Protection, National Research Council of Italy, 10135 Torino, ItalyDepartment of General Biology, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilDepartment of Pathogen Biology and Immunology, University of Wroclaw, 50-335 Wroclaw, PolandLaboratory of Molecular Immunovirology, Department of Microbiology, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilLaboratory of Gene Technology, Department of Biosystems, Division of Animal and Human Health Engineering, KU Leuven, 3000 Leuven, Belgium<i>Proteus mirabilis</i> is an opportunistic pathogen and is responsible for more than 40% of all cases of catheter-associated urinary tract infections (CAUTIs). Healthcare-associated infections have been aggravated by the constant emergence of antibiotic-resistant bacterial strains. Because of this, the use of phages to combat bacterial infections gained renewed interest. In this study, we describe the biological and genomic features of two <i>P. mirabilis</i> phages, named BigMira and MidiMira. These phages belong to the <i>Acadevirus</i> genus (family <i>Autographiviridae</i>). BigMira and MidiMira are highly similar, differing only in four missense mutations in their phage tail fiber. These mutations are sufficient to impact the phages’ depolymerase activity. Subsequently, the comparative genomic analysis of ten clinical <i>P. mirabilis</i> strains revealed differences in their antibiotic resistance profiles and lipopolysaccharide locus, with the latter potentially explaining the host range data of the phages. The massive presence of antimicrobial resistance genes, especially in the phages’ isolation strain <i>P. mirabilis</i> MCS, highlights the challenges in treating infections caused by multidrug-resistant bacteria. The findings reinforce BigMira and MidiMira phages as candidates for phage therapy purposes.https://www.mdpi.com/2076-2607/11/9/2141podoviruses<i>Acadevirus</i>depolymerasesCAUTI<i>Proteus mirabilis</i> |
| spellingShingle | Jéssica Duarte da Silva Lene Bens Adriele J. do Carmo Santos Rob Lavigne José Soares Luís D. R. Melo Marta Vallino Roberto Sousa Dias Zuzanna Drulis-Kawa Sérgio Oliveira de Paula Jeroen Wagemans Isolation and Characterization of the <i>Acadevirus</i> Members BigMira and MidiMira Infecting a Highly Pathogenic <i>Proteus mirabilis</i> Strain Microorganisms podoviruses <i>Acadevirus</i> depolymerases CAUTI <i>Proteus mirabilis</i> |
| title | Isolation and Characterization of the <i>Acadevirus</i> Members BigMira and MidiMira Infecting a Highly Pathogenic <i>Proteus mirabilis</i> Strain |
| title_full | Isolation and Characterization of the <i>Acadevirus</i> Members BigMira and MidiMira Infecting a Highly Pathogenic <i>Proteus mirabilis</i> Strain |
| title_fullStr | Isolation and Characterization of the <i>Acadevirus</i> Members BigMira and MidiMira Infecting a Highly Pathogenic <i>Proteus mirabilis</i> Strain |
| title_full_unstemmed | Isolation and Characterization of the <i>Acadevirus</i> Members BigMira and MidiMira Infecting a Highly Pathogenic <i>Proteus mirabilis</i> Strain |
| title_short | Isolation and Characterization of the <i>Acadevirus</i> Members BigMira and MidiMira Infecting a Highly Pathogenic <i>Proteus mirabilis</i> Strain |
| title_sort | isolation and characterization of the i acadevirus i members bigmira and midimira infecting a highly pathogenic i proteus mirabilis i strain |
| topic | podoviruses <i>Acadevirus</i> depolymerases CAUTI <i>Proteus mirabilis</i> |
| url | https://www.mdpi.com/2076-2607/11/9/2141 |
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