Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae)
The Theraphosidae (Thorell, 1869) is the most prevalent family in the suborder Mygalomorpae, but little is known about their cytogenetics, nor have the chromosomes of old-world tarantulas been studied. This study aims to develop an efficient protocol for chromosomal tarantula preparation in which ch...
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| Format: | Article |
| Language: | English |
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Mahasarakham University
2022-12-01
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| Series: | Science Technology and Engineering Journal (STEJ) |
| Subjects: | |
| Online Access: | https://ph02.tci-thaijo.org/index.php/stej/article/view/247428/168460 |
| _version_ | 1797902177040072704 |
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| author | Paveen Piyatrakulchai Alongklod Tanomtong Phichaya Buasriyot Narin Chomphuphuang |
| author_facet | Paveen Piyatrakulchai Alongklod Tanomtong Phichaya Buasriyot Narin Chomphuphuang |
| author_sort | Paveen Piyatrakulchai |
| collection | DOAJ |
| description | The Theraphosidae (Thorell, 1869) is the most prevalent family in the suborder Mygalomorpae, but little is known about their cytogenetics, nor have the chromosomes of old-world tarantulas been studied. This study aims to develop an efficient protocol for chromosomal tarantula preparation in which chromosomes are extracted directly from the internal organs of spiders following in vivo colchicine treatment. Chilobrachys (Karsch, 1892) and Cyriopagopus (Simon, 1888), which belong to different subfamilies, were chosen as validation models.The results indicate that the protocol for preparing chromosomes from both subfamilies is effective, suggesting that Southeast Asian tarantulas are probable to have bi-arms chromosomes and a high proportion of diploid number (2n). Interestingly, diploid chromosomes contain chromosomal gaps on many chromosomes, which will be investigated in the future to facilitate a greater understanding. |
| first_indexed | 2024-04-10T09:13:35Z |
| format | Article |
| id | doaj.art-cd6c9ca5106e429398541e60eb1bc33b |
| institution | Directory Open Access Journal |
| issn | 2697-6110 2697-6072 |
| language | English |
| last_indexed | 2024-04-10T09:13:35Z |
| publishDate | 2022-12-01 |
| publisher | Mahasarakham University |
| record_format | Article |
| series | Science Technology and Engineering Journal (STEJ) |
| spelling | doaj.art-cd6c9ca5106e429398541e60eb1bc33b2023-02-21T03:12:13ZengMahasarakham UniversityScience Technology and Engineering Journal (STEJ)2697-61102697-60722022-12-0182111118Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae)Paveen Piyatrakulchai0Alongklod Tanomtong1Phichaya Buasriyot2Narin Chomphuphuang3 Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, ThailandDepartment of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, ThailandAnimals Cytogenetics Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, ThailandDepartment of Entomology and Plant Pathology, Faculty of Agriculture, Khon Kaen University, Khon Kaen, ThailandThe Theraphosidae (Thorell, 1869) is the most prevalent family in the suborder Mygalomorpae, but little is known about their cytogenetics, nor have the chromosomes of old-world tarantulas been studied. This study aims to develop an efficient protocol for chromosomal tarantula preparation in which chromosomes are extracted directly from the internal organs of spiders following in vivo colchicine treatment. Chilobrachys (Karsch, 1892) and Cyriopagopus (Simon, 1888), which belong to different subfamilies, were chosen as validation models.The results indicate that the protocol for preparing chromosomes from both subfamilies is effective, suggesting that Southeast Asian tarantulas are probable to have bi-arms chromosomes and a high proportion of diploid number (2n). Interestingly, diploid chromosomes contain chromosomal gaps on many chromosomes, which will be investigated in the future to facilitate a greater understanding.https://ph02.tci-thaijo.org/index.php/stej/article/view/247428/168460chromosomechilobrachyscyriopagopusselenocosminaeornithoctoninae |
| spellingShingle | Paveen Piyatrakulchai Alongklod Tanomtong Phichaya Buasriyot Narin Chomphuphuang Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae) Science Technology and Engineering Journal (STEJ) chromosome chilobrachys cyriopagopus selenocosminae ornithoctoninae |
| title | Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae) |
| title_full | Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae) |
| title_fullStr | Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae) |
| title_full_unstemmed | Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae) |
| title_short | Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae) |
| title_sort | direct chromosomal preparation protocol from old world tarantulas araneae theraphosidae |
| topic | chromosome chilobrachys cyriopagopus selenocosminae ornithoctoninae |
| url | https://ph02.tci-thaijo.org/index.php/stej/article/view/247428/168460 |
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