Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neurons
Summary: Osmoregulation is an essential homeostatic process that requires constant release of vasopressin during sustained increases in plasma osmolality. The magnocellular neurosecretory cells (MNCs) respond to increases in external osmolality through increases in the activity of ΔN-TRPV1 channels,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223003358 |
| _version_ | 1811158467260448768 |
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| author | Kirk D. Haan Sung Jin Park Yoshikazu Nakamura Kiyoko Fukami Thomas E. Fisher |
| author_facet | Kirk D. Haan Sung Jin Park Yoshikazu Nakamura Kiyoko Fukami Thomas E. Fisher |
| author_sort | Kirk D. Haan |
| collection | DOAJ |
| description | Summary: Osmoregulation is an essential homeostatic process that requires constant release of vasopressin during sustained increases in plasma osmolality. The magnocellular neurosecretory cells (MNCs) respond to increases in external osmolality through increases in the activity of ΔN-TRPV1 channels, which leads to increased action potential firing and vasopressin release. We show that sustained exposure of acutely isolated rat and mouse MNCs to hypertonic solutions (90 min) causes a reversible translocation of ΔN-TRPV1 channels from internal stores to the plasma membrane that depends on the activation of phospholipase C and on SNARE-dependent exocytosis. ΔN-TRPV1 channel translocation is absent in MNCs isolated from transgenic mice lacking the PLCδ1 isoform, suggesting that PLCδ1 is essential for triggering this process. The translocation of ΔN-TRPV1 channels to the cell surface could contribute to the maintenance of MNC excitability during sustained increases in osmolality. Our data therefore have important implications for the mechanisms underlying mammalian osmoregulation. |
| first_indexed | 2024-04-10T05:24:05Z |
| format | Article |
| id | doaj.art-cd70fce54cd944daa951135da7ca96cc |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T05:24:05Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-cd70fce54cd944daa951135da7ca96cc2023-03-08T04:14:59ZengElsevieriScience2589-00422023-03-01263106258Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neuronsKirk D. Haan0Sung Jin Park1Yoshikazu Nakamura2Kiyoko Fukami3Thomas E. Fisher4Department of Anatomy, Physiology, and Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, SK, CanadaDepartment of Anatomy, Physiology, and Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, SK, CanadaDepartment of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, JapanLaboratory of Genome and Biosignals, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanDepartment of Anatomy, Physiology, and Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada; Corresponding authorSummary: Osmoregulation is an essential homeostatic process that requires constant release of vasopressin during sustained increases in plasma osmolality. The magnocellular neurosecretory cells (MNCs) respond to increases in external osmolality through increases in the activity of ΔN-TRPV1 channels, which leads to increased action potential firing and vasopressin release. We show that sustained exposure of acutely isolated rat and mouse MNCs to hypertonic solutions (90 min) causes a reversible translocation of ΔN-TRPV1 channels from internal stores to the plasma membrane that depends on the activation of phospholipase C and on SNARE-dependent exocytosis. ΔN-TRPV1 channel translocation is absent in MNCs isolated from transgenic mice lacking the PLCδ1 isoform, suggesting that PLCδ1 is essential for triggering this process. The translocation of ΔN-TRPV1 channels to the cell surface could contribute to the maintenance of MNC excitability during sustained increases in osmolality. Our data therefore have important implications for the mechanisms underlying mammalian osmoregulation.http://www.sciencedirect.com/science/article/pii/S2589004223003358Cellular neuroscienceCell biology |
| spellingShingle | Kirk D. Haan Sung Jin Park Yoshikazu Nakamura Kiyoko Fukami Thomas E. Fisher Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neurons iScience Cellular neuroscience Cell biology |
| title | Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neurons |
| title_full | Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neurons |
| title_fullStr | Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neurons |
| title_full_unstemmed | Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neurons |
| title_short | Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neurons |
| title_sort | osmotically evoked plcδ1 dependent translocation of δn trpv1 channels in rat supraoptic neurons |
| topic | Cellular neuroscience Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223003358 |
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