Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant Fever
Brucella bacterium causes Brucellosis, an infectious disease spreading from animals to human. Brucella lumazine synthase (BLS) is a highly immunogenic protein with adjuvant properties, which has been introduced as an effective protein carrier for vaccine development. This protein also plays a signif...
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| Language: | English |
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Razi Vaccine and Serum Research Institute
2019-03-01
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| Series: | Archives of Razi Institute |
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| Online Access: | http://archrazi.areeo.ac.ir/article_117997_0163597485faad89bb1bdbe5e65989ad.pdf |
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| author | R. Akbari M. H. Sekhavati A. Bahrami R. Majidzadeh Heravi S. Yousefi |
| author_facet | R. Akbari M. H. Sekhavati A. Bahrami R. Majidzadeh Heravi S. Yousefi |
| author_sort | R. Akbari |
| collection | DOAJ |
| description | Brucella bacterium causes Brucellosis, an infectious disease spreading from animals to human. Brucella lumazine synthase (BLS) is a highly immunogenic protein with adjuvant properties, which has been introduced as an effective protein carrier for vaccine development. This protein also plays a significant role in inducing immune system. This study aimed to clone, express, and purify the BLS gene from Brucella melitensis Rev1. The BLS gene was amplified by particular primers with the restriction enzyme sites as a linker and it was inserted into pTZ57R/T vector. Subsequently, it was ligated into pET32(a)+ expression vector. Recombinant expression vector containing coding sequence of BLS was transformed into E. coli BL21 (DE3) host gene expression and stimulated by 0.1mM IPTG. The results of sequencing showed that there were not any mutations in BLS encoding sequence. The expression results were set by sequencing and endorsed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses and western blotting that showed 35 kDa protein band appropriately. |
| first_indexed | 2024-04-13T12:57:02Z |
| format | Article |
| id | doaj.art-cd711317704341ada4be5d8f6817a3fb |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-04-13T12:57:02Z |
| publishDate | 2019-03-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-cd711317704341ada4be5d8f6817a3fb2022-12-22T02:46:01ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722019-03-017411610.22092/ari.2019.117997117997Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant FeverR. Akbari0M. H. Sekhavati1A. Bahrami2R. Majidzadeh Heravi3S. Yousefi4Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, IranImmunology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, IranBrucella bacterium causes Brucellosis, an infectious disease spreading from animals to human. Brucella lumazine synthase (BLS) is a highly immunogenic protein with adjuvant properties, which has been introduced as an effective protein carrier for vaccine development. This protein also plays a significant role in inducing immune system. This study aimed to clone, express, and purify the BLS gene from Brucella melitensis Rev1. The BLS gene was amplified by particular primers with the restriction enzyme sites as a linker and it was inserted into pTZ57R/T vector. Subsequently, it was ligated into pET32(a)+ expression vector. Recombinant expression vector containing coding sequence of BLS was transformed into E. coli BL21 (DE3) host gene expression and stimulated by 0.1mM IPTG. The results of sequencing showed that there were not any mutations in BLS encoding sequence. The expression results were set by sequencing and endorsed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses and western blotting that showed 35 kDa protein band appropriately.http://archrazi.areeo.ac.ir/article_117997_0163597485faad89bb1bdbe5e65989ad.pdfBrucella melitensis Rev1BLSGene ExpressionRecombinant Protein |
| spellingShingle | R. Akbari M. H. Sekhavati A. Bahrami R. Majidzadeh Heravi S. Yousefi Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant Fever Archives of Razi Institute Brucella melitensis Rev1 BLS Gene Expression Recombinant Protein |
| title | Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant Fever |
| title_full | Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant Fever |
| title_fullStr | Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant Fever |
| title_full_unstemmed | Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant Fever |
| title_short | Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant Fever |
| title_sort | production of brucella lumazine synthase recombinant protein to design a subunit vaccine against undulant fever |
| topic | Brucella melitensis Rev1 BLS Gene Expression Recombinant Protein |
| url | http://archrazi.areeo.ac.ir/article_117997_0163597485faad89bb1bdbe5e65989ad.pdf |
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