Impact of humic acids on EYL liposome membranes: ESR method
In this paper, the effects of model (commercial) and natural (extracted from peat) humic substances on the membrane of liposomes formed with egg yolk lecithin (EYL) are presented. In our research, mass concentrations of fulvic and humic acids were used, which in relation to lecithin varied from 0% t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2015-07-01
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| Series: | Nukleonika |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/nuka-2015-0081 |
| _version_ | 1798018283980455936 |
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| author | Pytel Barbara Filipiak Aneta Pisarek Izabella Olchawa Ryszard Man Dariusz |
| author_facet | Pytel Barbara Filipiak Aneta Pisarek Izabella Olchawa Ryszard Man Dariusz |
| author_sort | Pytel Barbara |
| collection | DOAJ |
| description | In this paper, the effects of model (commercial) and natural (extracted from peat) humic substances on the membrane of liposomes formed with egg yolk lecithin (EYL) are presented. In our research, mass concentrations of fulvic and humic acids were used, which in relation to lecithin varied from 0% to 13%. To study membrane fluidity, electron spin resonance (EPR) was used with two spin probes, penetrating various regions of the lipid bilayer. The effects of model and natural humic substances (humic acids – HAs and fulvic acids – FAs) on the lipid membrane in different regions were researched: the lipid-water interphase, and in the middle of the lipid bilayer. It was shown that FA and HA impact the fluidity of liposome membranes in different ways. Increased mass concentrations of HAs decreased membrane fluidity in both acids: extracted from peat and the model. However, increased mass concentration of FAs extracted from peat, decreased membrane fluidity in the surface region, at the same time stiffening the central part of the bilayer. Increasing the concentration of FAs extracted from peat had the opposite effect when compared to model FA. This effect may be related to the complexation of xenobiotics present in the soil environment and their impact on biological membranes. |
| first_indexed | 2024-04-11T16:21:32Z |
| format | Article |
| id | doaj.art-cd7175570b18462bb6f62566e4ca48e0 |
| institution | Directory Open Access Journal |
| issn | 0029-5922 |
| language | English |
| last_indexed | 2024-04-11T16:21:32Z |
| publishDate | 2015-07-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Nukleonika |
| spelling | doaj.art-cd7175570b18462bb6f62566e4ca48e02022-12-22T04:14:20ZengSciendoNukleonika0029-59222015-07-0160345545910.1515/nuka-2015-0081nuka-2015-0081Impact of humic acids on EYL liposome membranes: ESR methodPytel Barbara0Filipiak Aneta1Pisarek Izabella2Olchawa Ryszard3Man Dariusz4Institute of Physics, Opole University, 48 Oleska Str., 45-052 Opole, Poland, Tel.: +48 77 452 7250, Fax: +48 77 452 7290Department of Land Protection, Opole University, 22 Oleska Str., 45-052 Opole, PolandDepartment of Land Protection, Opole University, 22 Oleska Str., 45-052 Opole, PolandInstitute of Physics, Opole University, 48 Oleska Str., 45-052 Opole, Poland, Tel.: +48 77 452 7250, Fax: +48 77 452 7290Institute of Physics, Opole University, 48 Oleska Str., 45-052 Opole, Poland, Tel.: +48 77 452 7250, Fax: +48 77 452 7290In this paper, the effects of model (commercial) and natural (extracted from peat) humic substances on the membrane of liposomes formed with egg yolk lecithin (EYL) are presented. In our research, mass concentrations of fulvic and humic acids were used, which in relation to lecithin varied from 0% to 13%. To study membrane fluidity, electron spin resonance (EPR) was used with two spin probes, penetrating various regions of the lipid bilayer. The effects of model and natural humic substances (humic acids – HAs and fulvic acids – FAs) on the lipid membrane in different regions were researched: the lipid-water interphase, and in the middle of the lipid bilayer. It was shown that FA and HA impact the fluidity of liposome membranes in different ways. Increased mass concentrations of HAs decreased membrane fluidity in both acids: extracted from peat and the model. However, increased mass concentration of FAs extracted from peat, decreased membrane fluidity in the surface region, at the same time stiffening the central part of the bilayer. Increasing the concentration of FAs extracted from peat had the opposite effect when compared to model FA. This effect may be related to the complexation of xenobiotics present in the soil environment and their impact on biological membranes.https://doi.org/10.1515/nuka-2015-0081eyl liposomesesr methodhumic substances |
| spellingShingle | Pytel Barbara Filipiak Aneta Pisarek Izabella Olchawa Ryszard Man Dariusz Impact of humic acids on EYL liposome membranes: ESR method Nukleonika eyl liposomes esr method humic substances |
| title | Impact of humic acids on EYL liposome membranes: ESR method |
| title_full | Impact of humic acids on EYL liposome membranes: ESR method |
| title_fullStr | Impact of humic acids on EYL liposome membranes: ESR method |
| title_full_unstemmed | Impact of humic acids on EYL liposome membranes: ESR method |
| title_short | Impact of humic acids on EYL liposome membranes: ESR method |
| title_sort | impact of humic acids on eyl liposome membranes esr method |
| topic | eyl liposomes esr method humic substances |
| url | https://doi.org/10.1515/nuka-2015-0081 |
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