The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological Methods
Background: The water level of Poyang Lake (China) fluctuates seasonally. Shahu Lake, a smaller body of water connected to Poyang Lake during the wet season, is separated in the dry season. Due to a special fishing method termed ‘lake enclosed in autumn’, the water level is lowered and reaches its l...
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MDPI AG
2022-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/8/950 |
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| author | Xuemei Qiu Quanfeng Lu Chenchen Jia Yuting Dai Shan Ouyang Xiaoping Wu |
| author_facet | Xuemei Qiu Quanfeng Lu Chenchen Jia Yuting Dai Shan Ouyang Xiaoping Wu |
| author_sort | Xuemei Qiu |
| collection | DOAJ |
| description | Background: The water level of Poyang Lake (China) fluctuates seasonally. Shahu Lake, a smaller body of water connected to Poyang Lake during the wet season, is separated in the dry season. Due to a special fishing method termed ‘lake enclosed in autumn’, the water level is lowered and reaches its lowest point in January, which is <0.5 m deep in the middle of the lake. Our research investigated the effect of water level changes on the zooplankton community composition in Shahu Lake. Methods: We used both DNA metabarcoding method (MBC) (18S rRNA gene V4 region) and morphological method (MOI) to track the zooplankton community structure over four seasons in Shahu Lake (China). Results: Totals of 90 and 98 species of zooplankton were detected by MOI and MBC, respectively, with rotifers being the main zooplankton component. The α-diversity index of both methods increased from spring to summer and decreased from summer to autumn, reaching the lowest value in winter. NMDS and a cluster analysis showed that all zooplankton communities detected by MOI and MBC were significantly separated by season. The zooplankton community in winter was separated from that of the other three seasons, but the summer and autumn communities were more similar. Conclusions: Changes in the water level had significant effects on the zooplankton community composition. We found that MBC was more able to detect the differences in the zooplankton composition than MOI. MBC also had more advantages in copepod recognition. In our study, 37 species of copepods were detected by MBC, but only 11 species were detected by MOI. We concluded that MBC should be used to research the seasonal variations of zooplankton. |
| first_indexed | 2024-03-09T11:16:17Z |
| format | Article |
| id | doaj.art-cd77188707e44283b10ddd202c3d569c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T11:16:17Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-cd77188707e44283b10ddd202c3d569c2023-12-01T00:29:55ZengMDPI AGAnimals2076-26152022-04-0112895010.3390/ani12080950The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological MethodsXuemei Qiu0Quanfeng Lu1Chenchen Jia2Yuting Dai3Shan Ouyang4Xiaoping Wu5School of Life Science, Nanchang University, Nanchang 330036, ChinaSchool of Life Science, Nanchang University, Nanchang 330036, ChinaSchool of Life Science, Nanchang University, Nanchang 330036, ChinaSchool of Life Science, Nanchang University, Nanchang 330036, ChinaSchool of Life Science, Nanchang University, Nanchang 330036, ChinaSchool of Life Science, Nanchang University, Nanchang 330036, ChinaBackground: The water level of Poyang Lake (China) fluctuates seasonally. Shahu Lake, a smaller body of water connected to Poyang Lake during the wet season, is separated in the dry season. Due to a special fishing method termed ‘lake enclosed in autumn’, the water level is lowered and reaches its lowest point in January, which is <0.5 m deep in the middle of the lake. Our research investigated the effect of water level changes on the zooplankton community composition in Shahu Lake. Methods: We used both DNA metabarcoding method (MBC) (18S rRNA gene V4 region) and morphological method (MOI) to track the zooplankton community structure over four seasons in Shahu Lake (China). Results: Totals of 90 and 98 species of zooplankton were detected by MOI and MBC, respectively, with rotifers being the main zooplankton component. The α-diversity index of both methods increased from spring to summer and decreased from summer to autumn, reaching the lowest value in winter. NMDS and a cluster analysis showed that all zooplankton communities detected by MOI and MBC were significantly separated by season. The zooplankton community in winter was separated from that of the other three seasons, but the summer and autumn communities were more similar. Conclusions: Changes in the water level had significant effects on the zooplankton community composition. We found that MBC was more able to detect the differences in the zooplankton composition than MOI. MBC also had more advantages in copepod recognition. In our study, 37 species of copepods were detected by MBC, but only 11 species were detected by MOI. We concluded that MBC should be used to research the seasonal variations of zooplankton.https://www.mdpi.com/2076-2615/12/8/950ChinaDNA metabarcodingmorphological methodPoyang Lakezooplankton |
| spellingShingle | Xuemei Qiu Quanfeng Lu Chenchen Jia Yuting Dai Shan Ouyang Xiaoping Wu The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological Methods Animals China DNA metabarcoding morphological method Poyang Lake zooplankton |
| title | The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological Methods |
| title_full | The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological Methods |
| title_fullStr | The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological Methods |
| title_full_unstemmed | The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological Methods |
| title_short | The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological Methods |
| title_sort | effects of water level fluctuation on zooplankton communities in shahu lake based on dna metabarcoding and morphological methods |
| topic | China DNA metabarcoding morphological method Poyang Lake zooplankton |
| url | https://www.mdpi.com/2076-2615/12/8/950 |
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