Low potassium disrupt intestinal barrier and result in bacterial translocation
Abstract Background Bacterial translocation was observed in critical illness and patients with chronic diseases such as liver cirrhosis and chronic kidney disease (CKD). Hypokalemia is a common complication in these diseases. Whether low potassium diet may increase intestinal permeability and result...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12967-022-03499-0 |
| _version_ | 1828191824754245632 |
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| author | Haishan Wu Rong Huang Jinjin Fan Ning Luo Xiao Yang |
| author_facet | Haishan Wu Rong Huang Jinjin Fan Ning Luo Xiao Yang |
| author_sort | Haishan Wu |
| collection | DOAJ |
| description | Abstract Background Bacterial translocation was observed in critical illness and patients with chronic diseases such as liver cirrhosis and chronic kidney disease (CKD). Hypokalemia is a common complication in these diseases. Whether low potassium diet may increase intestinal permeability and result in bacterial translocation lack of evidence. The present study was aimed to investigate the potential effects of LK on intestinal permeability. Methods Grade 8-week-old male Bal B/C mice were randomly placed either on a normal potassium (NK) mouse chow or a low potassium (LK) diet for 28 days. Intestinal permeability and expression of tight junction proteins were compared between the two groups. Results Compared with the NK group, the mice in LK group had significantly lower serum potassium level, increased levels of plasmas endotoxin and plasma d-lactate. The bacterial translocation was higher and in occurred mainly in mesenteric lymph nodes (MLN), liver and spleen. The pathologic change of small intestine was obvious with thinner villus lamina propria, shorter crypt depth and thinner intestinal wall. Slight increases in the expression of proteins and mRNA levels of both claudin-1 and claudin-2 were observed in LK group. Conclusions Low potassium diet could increase intestinal permeability and thereby lead to bacterial translocation, which was suspected to result from impaired intestinal epithelial barrier and biological barrier. |
| first_indexed | 2024-04-12T08:45:30Z |
| format | Article |
| id | doaj.art-cd779a10837c4e6682ba51842f3aeff1 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-12T08:45:30Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-cd779a10837c4e6682ba51842f3aeff12022-12-22T03:39:43ZengBMCJournal of Translational Medicine1479-58762022-07-0120111110.1186/s12967-022-03499-0Low potassium disrupt intestinal barrier and result in bacterial translocationHaishan Wu0Rong Huang1Jinjin Fan2Ning Luo3Xiao Yang4Department of Nephrology, The First Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-Sen UniversityAbstract Background Bacterial translocation was observed in critical illness and patients with chronic diseases such as liver cirrhosis and chronic kidney disease (CKD). Hypokalemia is a common complication in these diseases. Whether low potassium diet may increase intestinal permeability and result in bacterial translocation lack of evidence. The present study was aimed to investigate the potential effects of LK on intestinal permeability. Methods Grade 8-week-old male Bal B/C mice were randomly placed either on a normal potassium (NK) mouse chow or a low potassium (LK) diet for 28 days. Intestinal permeability and expression of tight junction proteins were compared between the two groups. Results Compared with the NK group, the mice in LK group had significantly lower serum potassium level, increased levels of plasmas endotoxin and plasma d-lactate. The bacterial translocation was higher and in occurred mainly in mesenteric lymph nodes (MLN), liver and spleen. The pathologic change of small intestine was obvious with thinner villus lamina propria, shorter crypt depth and thinner intestinal wall. Slight increases in the expression of proteins and mRNA levels of both claudin-1 and claudin-2 were observed in LK group. Conclusions Low potassium diet could increase intestinal permeability and thereby lead to bacterial translocation, which was suspected to result from impaired intestinal epithelial barrier and biological barrier.https://doi.org/10.1186/s12967-022-03499-0Low potassiumGut permeabilityIntestinal barrierDysbacteriosisTight junction |
| spellingShingle | Haishan Wu Rong Huang Jinjin Fan Ning Luo Xiao Yang Low potassium disrupt intestinal barrier and result in bacterial translocation Journal of Translational Medicine Low potassium Gut permeability Intestinal barrier Dysbacteriosis Tight junction |
| title | Low potassium disrupt intestinal barrier and result in bacterial translocation |
| title_full | Low potassium disrupt intestinal barrier and result in bacterial translocation |
| title_fullStr | Low potassium disrupt intestinal barrier and result in bacterial translocation |
| title_full_unstemmed | Low potassium disrupt intestinal barrier and result in bacterial translocation |
| title_short | Low potassium disrupt intestinal barrier and result in bacterial translocation |
| title_sort | low potassium disrupt intestinal barrier and result in bacterial translocation |
| topic | Low potassium Gut permeability Intestinal barrier Dysbacteriosis Tight junction |
| url | https://doi.org/10.1186/s12967-022-03499-0 |
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