A novel animal model of osteonecrosis of the femoral head based on 3D printing technology
Abstract Background Osteonecrosis of the femoral head (ONFH) is a prevalent orthopedic condition characterized by the disruption of blood supply to the femoral head, leading to ischemia of internal tissues, subchondral bone fractures, necrosis, and eventual collapse of the weight-bearing portion of...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-08-01
|
| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-023-04050-7 |
| _version_ | 1797452065072480256 |
|---|---|
| author | Yiyang Li Jiewen Zhang Yiwei Zhao Run Tian Pei Yang |
| author_facet | Yiyang Li Jiewen Zhang Yiwei Zhao Run Tian Pei Yang |
| author_sort | Yiyang Li |
| collection | DOAJ |
| description | Abstract Background Osteonecrosis of the femoral head (ONFH) is a prevalent orthopedic condition characterized by the disruption of blood supply to the femoral head, leading to ischemia of internal tissues, subchondral bone fractures, necrosis, and eventual collapse of the weight-bearing portion of the femoral head. This condition results in severe functional impairment, pain, and even disability of the hip joint. Existing animal models of ONFH have limitations in replicating the natural disease progression accurately. Thus, there is a critical need to develop a novel animal model capable of better simulating localized pressure on the human femoral head to facilitate ONFH-related research. Methods In this study, we present a novel approach for modeling ONFH, which involves integrating stress factors into the modeling process through the utilization of 3D printing technology and principles of biomechanics. A total of 36 animals were randomly assigned to six groups, where they received either the novel modeling technique or the traditional hormone induction method. Subsequently, an 8-week treatment period was implemented, followed by conducting micro-CT scans and histological evaluations to assess tissue outcomes. Results The study evaluated the cytotoxicity of the material used in the new model, and it was observed that the material did not exhibit any cytotoxic effects on cells. Additionally, the novel model successfully replicated the pathological features of ONFH, including femoral head collapse, along with a substantial presence of empty bone lacunae, cartilage defects, and subchondral bone fractures in the subchondral bone region. Conclusion In conclusion, our study provides evidence that the new model shows the ability to simulate the progression of the disease, making it a valuable tool for research in this field and can contribute to the development of better treatment strategies for this debilitating condition. It holds great promise for advancing our understanding of the pathogenesis of ONFH and the potential therapeutic interventions for this challenging clinical problem. |
| first_indexed | 2024-03-09T15:03:22Z |
| format | Article |
| id | doaj.art-cd785326efaf4b8d831ac22ad8409900 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-03-09T15:03:22Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-cd785326efaf4b8d831ac22ad84099002023-11-26T13:48:40ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2023-08-0118111210.1186/s13018-023-04050-7A novel animal model of osteonecrosis of the femoral head based on 3D printing technologyYiyang Li0Jiewen Zhang1Yiwei Zhao2Run Tian3Pei Yang4Second Affiliated Hospital of Xi’an Jiaotong UniversitySecond Affiliated Hospital of Xi’an Jiaotong UniversitySecond Affiliated Hospital of Xi’an Jiaotong UniversitySecond Affiliated Hospital of Xi’an Jiaotong UniversitySecond Affiliated Hospital of Xi’an Jiaotong UniversityAbstract Background Osteonecrosis of the femoral head (ONFH) is a prevalent orthopedic condition characterized by the disruption of blood supply to the femoral head, leading to ischemia of internal tissues, subchondral bone fractures, necrosis, and eventual collapse of the weight-bearing portion of the femoral head. This condition results in severe functional impairment, pain, and even disability of the hip joint. Existing animal models of ONFH have limitations in replicating the natural disease progression accurately. Thus, there is a critical need to develop a novel animal model capable of better simulating localized pressure on the human femoral head to facilitate ONFH-related research. Methods In this study, we present a novel approach for modeling ONFH, which involves integrating stress factors into the modeling process through the utilization of 3D printing technology and principles of biomechanics. A total of 36 animals were randomly assigned to six groups, where they received either the novel modeling technique or the traditional hormone induction method. Subsequently, an 8-week treatment period was implemented, followed by conducting micro-CT scans and histological evaluations to assess tissue outcomes. Results The study evaluated the cytotoxicity of the material used in the new model, and it was observed that the material did not exhibit any cytotoxic effects on cells. Additionally, the novel model successfully replicated the pathological features of ONFH, including femoral head collapse, along with a substantial presence of empty bone lacunae, cartilage defects, and subchondral bone fractures in the subchondral bone region. Conclusion In conclusion, our study provides evidence that the new model shows the ability to simulate the progression of the disease, making it a valuable tool for research in this field and can contribute to the development of better treatment strategies for this debilitating condition. It holds great promise for advancing our understanding of the pathogenesis of ONFH and the potential therapeutic interventions for this challenging clinical problem.https://doi.org/10.1186/s13018-023-04050-7Animal modelOsteonecrosis of the femoral headRabbit3D printing technology |
| spellingShingle | Yiyang Li Jiewen Zhang Yiwei Zhao Run Tian Pei Yang A novel animal model of osteonecrosis of the femoral head based on 3D printing technology Journal of Orthopaedic Surgery and Research Animal model Osteonecrosis of the femoral head Rabbit 3D printing technology |
| title | A novel animal model of osteonecrosis of the femoral head based on 3D printing technology |
| title_full | A novel animal model of osteonecrosis of the femoral head based on 3D printing technology |
| title_fullStr | A novel animal model of osteonecrosis of the femoral head based on 3D printing technology |
| title_full_unstemmed | A novel animal model of osteonecrosis of the femoral head based on 3D printing technology |
| title_short | A novel animal model of osteonecrosis of the femoral head based on 3D printing technology |
| title_sort | novel animal model of osteonecrosis of the femoral head based on 3d printing technology |
| topic | Animal model Osteonecrosis of the femoral head Rabbit 3D printing technology |
| url | https://doi.org/10.1186/s13018-023-04050-7 |
| work_keys_str_mv | AT yiyangli anovelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT jiewenzhang anovelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT yiweizhao anovelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT runtian anovelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT peiyang anovelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT yiyangli novelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT jiewenzhang novelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT yiweizhao novelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT runtian novelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology AT peiyang novelanimalmodelofosteonecrosisofthefemoralheadbasedon3dprintingtechnology |