Eucalyptus oil abrogated liver damage, oxidant /antioxidant imbalance, inflammation and apoptosis stimulated by acetaminophen in rats: biochemical, molecular and histological approaches
Acetaminophen is an analgesic antipyretic commonly used. Hepatotoxicity is one of the most common obstacles to acetaminophen therapy. Eucalyptus oil is an antioxidant with potent free radical-capturing activities. This research was designed to evaluate the inhibitory impact of Eucalyptus oil versus...
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| Format: | Article |
| Language: | English |
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Assiut University
2024-04-01
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| Series: | Journal of Advanced Veterinary Research |
| Subjects: | |
| Online Access: | https://www.advetresearch.com/index.php/AVR/article/view/1796 |
| _version_ | 1797214715057799168 |
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| author | Noran W. Esmail Sawsan M. El-sheikh Reda M.S. Korany Arwa A. Hassan Sara M. Baraka Esraa M. Fahmy Doaa A. Mansour |
| author_facet | Noran W. Esmail Sawsan M. El-sheikh Reda M.S. Korany Arwa A. Hassan Sara M. Baraka Esraa M. Fahmy Doaa A. Mansour |
| author_sort | Noran W. Esmail |
| collection | DOAJ |
| description |
Acetaminophen is an analgesic antipyretic commonly used. Hepatotoxicity is one of the most common obstacles to acetaminophen therapy. Eucalyptus oil is an antioxidant with potent free radical-capturing activities. This research was designed to evaluate the inhibitory impact of Eucalyptus oil versus acetaminophen-triggered hepatotoxicity. The composition of Eucalyptus was detected utilizing gas chromatography mass spectroscopy. Forty-eight rats were assigned into six groups; control group, acetaminophen group (500mg/kg I.P twice on the 17th and, 20th of the experiment), silymarin group (50mg/kg, I.P once daily for three weeks), Eucalyptus oil group (30mg/kg orally once daily for three weeks), acetaminophen +silymarin group and Eucalyptus +acetaminophen group. gas chromatography mass spectroscopy discovered four compounds in Eucalyptus with eucalyptol representing the main compound. revealed that acetaminophen remarkably elevated serum alanine transaminase, aspartate aminotransferase, alkaline phosphatase, and total bilirubin levels, whereas it declined serum albumin and total protein levels. In addition, hepatic oxidant/antioxidant imbalance was evident in acetaminophen-intoxicated rats by the rising of lipid peroxidation biomarker; malondialdehyde and the downregulation of nuclear factor erythroid 2-related factor 2 and its transcriptional mediators; superoxide dismutase, glutathione peroxidase, and reduced glutathione. Furthermore, following acetaminophen injection, there was a remarkable increase in transforming growth factor-β gene expression, tumor necrosis factor-α and interleukin1-β levels along with a decline in interleukin-10 levels. Immunohistochemical and histopathological examinations were in parallel with the abovementioned results. However, all these abnormalities were significantly abrogated in rats pretreated with Eucalyptus. We conclude that prior administration of Eucalyptus oil counteracted acetaminophen-mediated hepatotoxicity via powerful antioxidant, anti-inflammatory, and anti-apoptotic impacts.
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| first_indexed | 2024-04-24T11:18:34Z |
| format | Article |
| id | doaj.art-cd79c36cb6b24f588d253b508a9edfa1 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-04-24T11:18:34Z |
| publishDate | 2024-04-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-cd79c36cb6b24f588d253b508a9edfa12024-04-11T05:14:42ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772024-04-01144Eucalyptus oil abrogated liver damage, oxidant /antioxidant imbalance, inflammation and apoptosis stimulated by acetaminophen in rats: biochemical, molecular and histological approachesNoran W. Esmail0Sawsan M. El-sheikh1Reda M.S. Korany2Arwa A. Hassan3Sara M. Baraka4Esraa M. Fahmy5Doaa A. Mansour6Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.Department of Pharmacology and Toxicology, Ministry of Health & Population, Egypt.Department of Chemistry of Natural Compounds, National Research Centre, Giza 12622, Egypt.Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Egypt. Acetaminophen is an analgesic antipyretic commonly used. Hepatotoxicity is one of the most common obstacles to acetaminophen therapy. Eucalyptus oil is an antioxidant with potent free radical-capturing activities. This research was designed to evaluate the inhibitory impact of Eucalyptus oil versus acetaminophen-triggered hepatotoxicity. The composition of Eucalyptus was detected utilizing gas chromatography mass spectroscopy. Forty-eight rats were assigned into six groups; control group, acetaminophen group (500mg/kg I.P twice on the 17th and, 20th of the experiment), silymarin group (50mg/kg, I.P once daily for three weeks), Eucalyptus oil group (30mg/kg orally once daily for three weeks), acetaminophen +silymarin group and Eucalyptus +acetaminophen group. gas chromatography mass spectroscopy discovered four compounds in Eucalyptus with eucalyptol representing the main compound. revealed that acetaminophen remarkably elevated serum alanine transaminase, aspartate aminotransferase, alkaline phosphatase, and total bilirubin levels, whereas it declined serum albumin and total protein levels. In addition, hepatic oxidant/antioxidant imbalance was evident in acetaminophen-intoxicated rats by the rising of lipid peroxidation biomarker; malondialdehyde and the downregulation of nuclear factor erythroid 2-related factor 2 and its transcriptional mediators; superoxide dismutase, glutathione peroxidase, and reduced glutathione. Furthermore, following acetaminophen injection, there was a remarkable increase in transforming growth factor-β gene expression, tumor necrosis factor-α and interleukin1-β levels along with a decline in interleukin-10 levels. Immunohistochemical and histopathological examinations were in parallel with the abovementioned results. However, all these abnormalities were significantly abrogated in rats pretreated with Eucalyptus. We conclude that prior administration of Eucalyptus oil counteracted acetaminophen-mediated hepatotoxicity via powerful antioxidant, anti-inflammatory, and anti-apoptotic impacts. https://www.advetresearch.com/index.php/AVR/article/view/1796AcetaminophenSilymarinEucalyptus OilLiver injuryOxidationinflammation |
| spellingShingle | Noran W. Esmail Sawsan M. El-sheikh Reda M.S. Korany Arwa A. Hassan Sara M. Baraka Esraa M. Fahmy Doaa A. Mansour Eucalyptus oil abrogated liver damage, oxidant /antioxidant imbalance, inflammation and apoptosis stimulated by acetaminophen in rats: biochemical, molecular and histological approaches Journal of Advanced Veterinary Research Acetaminophen Silymarin Eucalyptus Oil Liver injury Oxidation inflammation |
| title | Eucalyptus oil abrogated liver damage, oxidant /antioxidant imbalance, inflammation and apoptosis stimulated by acetaminophen in rats: biochemical, molecular and histological approaches |
| title_full | Eucalyptus oil abrogated liver damage, oxidant /antioxidant imbalance, inflammation and apoptosis stimulated by acetaminophen in rats: biochemical, molecular and histological approaches |
| title_fullStr | Eucalyptus oil abrogated liver damage, oxidant /antioxidant imbalance, inflammation and apoptosis stimulated by acetaminophen in rats: biochemical, molecular and histological approaches |
| title_full_unstemmed | Eucalyptus oil abrogated liver damage, oxidant /antioxidant imbalance, inflammation and apoptosis stimulated by acetaminophen in rats: biochemical, molecular and histological approaches |
| title_short | Eucalyptus oil abrogated liver damage, oxidant /antioxidant imbalance, inflammation and apoptosis stimulated by acetaminophen in rats: biochemical, molecular and histological approaches |
| title_sort | eucalyptus oil abrogated liver damage oxidant antioxidant imbalance inflammation and apoptosis stimulated by acetaminophen in rats biochemical molecular and histological approaches |
| topic | Acetaminophen Silymarin Eucalyptus Oil Liver injury Oxidation inflammation |
| url | https://www.advetresearch.com/index.php/AVR/article/view/1796 |
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