| Summary: | The <i>DA1/DAR</i> family genes have been shown to play important roles in regulating organ size and plant biomass in the model plant Arabidopsis and several crops. However, this family has not been characterized in <i>Brassica rapa</i> (<i>B. rapa</i>). In this study, we identified 17 <i>DA1</i>&<i>DAR</i> genes from <i>B. rapa</i>. Phylogenetic analysis indicated that these genes are classified into four groups. Structural and motif analysis of <i>BrDA1</i>&<i>DARs</i> discovered that the genes within the same group have similar exon-intron structures and share an equal number of conserved motifs except for <i>BrDAR6.3</i> from group IV, which contains two conserved motifs. <i>Cis</i>-regulatory elements identified four phytohormones (Salicylic acid, Abscisic acid, Gibberellin, and auxin) and three major abiotic (Light, Low temperature, and drought) responsive elements. Further, six br-miRNAs named br-miR164a, br-miR164b, br-miR164c, br-miR164d, br-miRN360, and br-miRN366 were found which target <i>BrDAR6.1</i>, <i>BrDA1.4</i>, and <i>BrDA1.5</i>. <i>BrDA1</i>&<i>DAR</i> genes were highly expressed in stem, root, silique, flower, leaf, and callus tissues. Moreover, qRT-PCR analyses indicated that some of these genes were responsive to abiotic stresses or phytohormone treatments. Our findings provide a foundation for further genetic and physiological studies of <i>BrDA1</i>&<i>DARs</i> in <i>B. rapa</i>.
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