Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening
The movement away from mammalian testing of potential toxicants and new chemical entities has primarily led to cell line testing and protein-based assays. However, these assays may not yet be sufficient to properly characterize the toxic potential of a chemical. The zebrafish embryo model is widely...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Toxics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2305-6304/8/4/126 |
| _version_ | 1797544027561656320 |
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| author | John C. Achenbach Cindy Leggiadro Sandra A. Sperker Cindy Woodland Lee D. Ellis |
| author_facet | John C. Achenbach Cindy Leggiadro Sandra A. Sperker Cindy Woodland Lee D. Ellis |
| author_sort | John C. Achenbach |
| collection | DOAJ |
| description | The movement away from mammalian testing of potential toxicants and new chemical entities has primarily led to cell line testing and protein-based assays. However, these assays may not yet be sufficient to properly characterize the toxic potential of a chemical. The zebrafish embryo model is widely recognized as a potential new approach method for chemical testing that may provide a bridge between cell and protein-based assays and mammalian testing. The Zebrafish Embryo Toxicity (ZET) model is increasingly recognized as a valuable toxicity testing platform. The ZET assay focuses on the early stages of embryo development and is considered a more humane model compared to adult zebrafish testing. A complementary model has been developed that exposes larvae to toxicants at a later time point during development where body patterning has already been established. Here we compare the toxicity profiles of 20 compounds for this General and Behavioral Toxicity (GBT) assay to the ZET assay. The results show partially overlapping toxicity profiles along with unique information provided by each assay. It appears from this work that these two assays applied together can strengthen the use of zebrafish embryos/larvae as standard toxicity testing models. |
| first_indexed | 2024-03-10T13:53:44Z |
| format | Article |
| id | doaj.art-cd7aa085e4f641eea0aa656f6f43ec6c |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T13:53:44Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-cd7aa085e4f641eea0aa656f6f43ec6c2023-11-21T01:53:03ZengMDPI AGToxics2305-63042020-12-018412610.3390/toxics8040126Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical ScreeningJohn C. Achenbach0Cindy Leggiadro1Sandra A. Sperker2Cindy Woodland3Lee D. Ellis4Aquatic and Crop Resource Development, National Research Council of Canada, Halifax, NS B3H 3Z1, CanadaAquatic and Crop Resource Development, National Research Council of Canada, Halifax, NS B3H 3Z1, CanadaAquatic and Crop Resource Development, National Research Council of Canada, Halifax, NS B3H 3Z1, CanadaNew Substances Assessment Control Bureau, Health Canada, Ottawa, ON K1A 0K9, CanadaNew Substances Assessment Control Bureau, Health Canada, Ottawa, ON K1A 0K9, CanadaThe movement away from mammalian testing of potential toxicants and new chemical entities has primarily led to cell line testing and protein-based assays. However, these assays may not yet be sufficient to properly characterize the toxic potential of a chemical. The zebrafish embryo model is widely recognized as a potential new approach method for chemical testing that may provide a bridge between cell and protein-based assays and mammalian testing. The Zebrafish Embryo Toxicity (ZET) model is increasingly recognized as a valuable toxicity testing platform. The ZET assay focuses on the early stages of embryo development and is considered a more humane model compared to adult zebrafish testing. A complementary model has been developed that exposes larvae to toxicants at a later time point during development where body patterning has already been established. Here we compare the toxicity profiles of 20 compounds for this General and Behavioral Toxicity (GBT) assay to the ZET assay. The results show partially overlapping toxicity profiles along with unique information provided by each assay. It appears from this work that these two assays applied together can strengthen the use of zebrafish embryos/larvae as standard toxicity testing models.https://www.mdpi.com/2305-6304/8/4/126zebrafish larvaetoxicity assayphenotypedevelopmental period |
| spellingShingle | John C. Achenbach Cindy Leggiadro Sandra A. Sperker Cindy Woodland Lee D. Ellis Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening Toxics zebrafish larvae toxicity assay phenotype developmental period |
| title | Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening |
| title_full | Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening |
| title_fullStr | Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening |
| title_full_unstemmed | Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening |
| title_short | Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening |
| title_sort | comparison of the zebrafish embryo toxicity assay and the general and behavioral embryo toxicity assay as new approach methods for chemical screening |
| topic | zebrafish larvae toxicity assay phenotype developmental period |
| url | https://www.mdpi.com/2305-6304/8/4/126 |
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