Preparation, Optimization and In-Vitro Evaluation of Curcumin-Loaded Niosome@calcium Alginate Nanocarrier as a New Approach for Breast Cancer Treatment
Cancer is one of the most common causes of mortality, and its various treatment methods can have many challenges for patients. As one of the most widely used cancer treatments, chemotherapy may result in diverse side effects. The lack of targeted drug delivery to tumor tissues can raise the possibil...
| المؤلفون الرئيسيون: | , , , , , , , |
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| التنسيق: | مقال |
| اللغة: | English |
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MDPI AG
2021-02-01
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| سلاسل: | Biology |
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| الوصول للمادة أونلاين: | https://www.mdpi.com/2079-7737/10/3/173 |
| _version_ | 1827588255667716096 |
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| author | Iman Akbarzadeh Mona Shayan Mahsa Bourbour Maryam Moghtaderi Hassan Noorbazargan Faten Eshrati Yeganeh Samaneh Saffar Mohammadreza Tahriri |
| author_facet | Iman Akbarzadeh Mona Shayan Mahsa Bourbour Maryam Moghtaderi Hassan Noorbazargan Faten Eshrati Yeganeh Samaneh Saffar Mohammadreza Tahriri |
| author_sort | Iman Akbarzadeh |
| collection | DOAJ |
| description | Cancer is one of the most common causes of mortality, and its various treatment methods can have many challenges for patients. As one of the most widely used cancer treatments, chemotherapy may result in diverse side effects. The lack of targeted drug delivery to tumor tissues can raise the possibility of damage to healthy tissues, with attendant dysfunction. In the present study, an optimum formulation of curcumin-loaded niosomes with a calcium alginate shell (AL-NioC) was developed and optimized by a three-level Box–Behnken design—in terms of dimension and drug loading efficiency. The niosomes were characterized by transmission electron microscopy, Fourier-transform infrared spectroscopy, and dynamic light scattering. The as-formulated niosomes showed excellent stability for up to 1 month at 4 °C. Additionally, the niosomal formulation demonstrated a pH-dependent release; a slow-release profile in physiological pH (7.4), and a more significant release rate at acidic conditions (pH = 3). Cytotoxicity studies showed high compatibility of AL-NioC toward normal MCF10A cells, while significant toxicity was observed in MDA-MB-231 and SKBR3 breast cancer cells. Gene expression studies of the cancer cells showed downregulation of Bcl2, cyclin D, and cyclin E genes, as well as upregulation of P53, Bax, caspase-3, and caspase-9 genes expression following the designed treatment. Flow cytometry studies confirmed a significant enhancement in the apoptosis rate in the presence of AL-NioC in both MDA-MB-231 and SKBR3 cells as compared to other samples. In general, the results of this study demonstrated that—thanks to its biocompatibility toward normal cells—the AL-NioC formulation can efficiently deliver hydrophobic drugs to target cancer cells while reducing side effects. |
| first_indexed | 2024-03-09T00:30:54Z |
| format | Article |
| id | doaj.art-cd7ac1e7ee91490fbb6d6db7e3e4ccd8 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T00:30:54Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-cd7ac1e7ee91490fbb6d6db7e3e4ccd82023-12-11T18:31:37ZengMDPI AGBiology2079-77372021-02-0110317310.3390/biology10030173Preparation, Optimization and In-Vitro Evaluation of Curcumin-Loaded Niosome@calcium Alginate Nanocarrier as a New Approach for Breast Cancer TreatmentIman Akbarzadeh0Mona Shayan1Mahsa Bourbour2Maryam Moghtaderi3Hassan Noorbazargan4Faten Eshrati Yeganeh5Samaneh Saffar6Mohammadreza Tahriri7Department of Chemical and Petrochemical Engineering, Sharif University of Technology, Tehran 1458889694, IranCore Facility Center, Pasteur Institute of Iran, Tehran 1316943551, IranDepartment of Biotechnology, Alzahra University, Tehran 1993893973, IranDepartment of Chemical Engineering, Faculty of Engineering, University of Tehran, Tehran 141556619, IranDepartment of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1985717443, IranDepartment of Chemistry, Science and Research Branch, Islamic Azad University, Tehran 1477893855, IranCore Facility Center, Pasteur Institute of Iran, Tehran 1316943551, IranDepartment of Engineering, Norfolk state University, Norfolk, VA 23504, USACancer is one of the most common causes of mortality, and its various treatment methods can have many challenges for patients. As one of the most widely used cancer treatments, chemotherapy may result in diverse side effects. The lack of targeted drug delivery to tumor tissues can raise the possibility of damage to healthy tissues, with attendant dysfunction. In the present study, an optimum formulation of curcumin-loaded niosomes with a calcium alginate shell (AL-NioC) was developed and optimized by a three-level Box–Behnken design—in terms of dimension and drug loading efficiency. The niosomes were characterized by transmission electron microscopy, Fourier-transform infrared spectroscopy, and dynamic light scattering. The as-formulated niosomes showed excellent stability for up to 1 month at 4 °C. Additionally, the niosomal formulation demonstrated a pH-dependent release; a slow-release profile in physiological pH (7.4), and a more significant release rate at acidic conditions (pH = 3). Cytotoxicity studies showed high compatibility of AL-NioC toward normal MCF10A cells, while significant toxicity was observed in MDA-MB-231 and SKBR3 breast cancer cells. Gene expression studies of the cancer cells showed downregulation of Bcl2, cyclin D, and cyclin E genes, as well as upregulation of P53, Bax, caspase-3, and caspase-9 genes expression following the designed treatment. Flow cytometry studies confirmed a significant enhancement in the apoptosis rate in the presence of AL-NioC in both MDA-MB-231 and SKBR3 cells as compared to other samples. In general, the results of this study demonstrated that—thanks to its biocompatibility toward normal cells—the AL-NioC formulation can efficiently deliver hydrophobic drugs to target cancer cells while reducing side effects.https://www.mdpi.com/2079-7737/10/3/173niosomecalcium alginatecurcuminbreast canceranticancer |
| spellingShingle | Iman Akbarzadeh Mona Shayan Mahsa Bourbour Maryam Moghtaderi Hassan Noorbazargan Faten Eshrati Yeganeh Samaneh Saffar Mohammadreza Tahriri Preparation, Optimization and In-Vitro Evaluation of Curcumin-Loaded Niosome@calcium Alginate Nanocarrier as a New Approach for Breast Cancer Treatment Biology niosome calcium alginate curcumin breast cancer anticancer |
| title | Preparation, Optimization and In-Vitro Evaluation of Curcumin-Loaded Niosome@calcium Alginate Nanocarrier as a New Approach for Breast Cancer Treatment |
| title_full | Preparation, Optimization and In-Vitro Evaluation of Curcumin-Loaded Niosome@calcium Alginate Nanocarrier as a New Approach for Breast Cancer Treatment |
| title_fullStr | Preparation, Optimization and In-Vitro Evaluation of Curcumin-Loaded Niosome@calcium Alginate Nanocarrier as a New Approach for Breast Cancer Treatment |
| title_full_unstemmed | Preparation, Optimization and In-Vitro Evaluation of Curcumin-Loaded Niosome@calcium Alginate Nanocarrier as a New Approach for Breast Cancer Treatment |
| title_short | Preparation, Optimization and In-Vitro Evaluation of Curcumin-Loaded Niosome@calcium Alginate Nanocarrier as a New Approach for Breast Cancer Treatment |
| title_sort | preparation optimization and in vitro evaluation of curcumin loaded niosome calcium alginate nanocarrier as a new approach for breast cancer treatment |
| topic | niosome calcium alginate curcumin breast cancer anticancer |
| url | https://www.mdpi.com/2079-7737/10/3/173 |
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