Exogenous γ-aminobutyric acid (GABA) mitigated salinity-induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potential
BackgroundIncreasing soil salinization has a detrimental effect on agricultural productivity.Therefore, strategies are needed to induce salinity-tolerance in crop species for sustainable foodproduction. γ-aminobutyric acid (GABA) plays a key role in regulating plant salinity stresstolerance. However...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1081188/full |
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| author | Abd Ullah Abd Ullah Abd Ullah Iftikhar Ali Iftikhar Ali Javaria Noor Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng Sami Bawazeer Sayed M Eldin Muhammad Ahsan Asghar Hafiz Hassan Javed Khansa Saleem Sami Ullah Haider Ali |
| author_facet | Abd Ullah Abd Ullah Abd Ullah Iftikhar Ali Iftikhar Ali Javaria Noor Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng Sami Bawazeer Sayed M Eldin Muhammad Ahsan Asghar Hafiz Hassan Javed Khansa Saleem Sami Ullah Haider Ali |
| author_sort | Abd Ullah |
| collection | DOAJ |
| description | BackgroundIncreasing soil salinization has a detrimental effect on agricultural productivity.Therefore, strategies are needed to induce salinity-tolerance in crop species for sustainable foodproduction. γ-aminobutyric acid (GABA) plays a key role in regulating plant salinity stresstolerance. However, it remains largely unknown how mungbean plants (Vigna radiata L.) respondto exogenous GABA under salinity stress.MethodsThus, we evaluated the effect of exogenous GABA (1.5 mM) on the growth and physiobiochemicalresponse mechanism of mungbean plants to saline stress (0-, 50-, and 100 mM [NaCland Na2SO4, at a 1:1 molar ratio]).ResultsIncreased saline stress adversely affected mungbean plants' growth and metabolism. Forinstance, leaf-stem-root biomass (34- and 56%, 31- and 53%, and 27- and 56% under 50- and 100mM, respectively]) and chlorophyll concentrations declined. The carotenoid level increased (10%)at 50 mM and remained unaffected at 100 mM. Hydrogen peroxide (H2O2), malondialdehyde(MDA), osmolytes (soluble sugars, soluble proteins, proline), total phenolic content, andenzymatic activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase(POD), glutathione reductase (GTR), and polyphenol oxidation (PPO) were significantlyincreased. In leaves, salinity caused a significant increase in Na+ concentration but a decrease inK+ concentration, resulting in a low K+/Na+ concentration (51- and 71% under 50- and 100- mMstress). Additionally, nitrogen concentration and the activities of nitrate reductase (NR) andglutamine synthetase (GS) decreased significantly. The reduction in glutamate synthase (GOGAT)activity was only significant (65%) at 100 mM stress. Exogenous GABA decreased Na+, H2O2,and MDA concentrations but enhanced photosynthetic pigments, K+ and K+/Na+ ratio, Nmetabolism, osmolytes, and enzymatic antioxidant activities, thus reducing salinity-associatedstress damages, resulting in improved growth and biomass.ConclusionExogenous GABA may have improved the salinity tolerance of mungbean plants by maintaining their morpho-physiological responses and reducing the accumulation of harmfulsubstances under salinity. Future molecular studies can contribute to a better understanding of themolecular mechanisms by which GABA regulates mungbean salinity tolerance. |
| first_indexed | 2024-04-10T21:13:31Z |
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| language | English |
| last_indexed | 2024-04-10T21:13:31Z |
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| spelling | doaj.art-cd81c1d0453042659d109baad60465fb2023-01-20T17:02:05ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10811881081188Exogenous γ-aminobutyric acid (GABA) mitigated salinity-induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potentialAbd Ullah0Abd Ullah1Abd Ullah2Iftikhar Ali3Iftikhar Ali4Javaria Noor5Fanjiang Zeng6Fanjiang Zeng7Fanjiang Zeng8Sami Bawazeer9Sayed M Eldin10Muhammad Ahsan Asghar11Hafiz Hassan Javed12Khansa Saleem13Sami Ullah14Haider Ali15Xinjiang Key Laboratory of Desert Plant Root Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaCele National Station of Observation and Research for Desert-Grassland Ecosystems, Cele, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCenter for Plant Sciences and Biodiversity, University of Swat, Charbagh Swat, PakistanDepartment of Genetics and Development, Columbia University Irving Medical Center, New York, NY, United StatesDepartment of Botany, Islamia College University, Peshawar, PakistanXinjiang Key Laboratory of Desert Plant Root Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaCele National Station of Observation and Research for Desert-Grassland Ecosystems, Cele, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaUmm Al-Qura University, Faculty of Pharmacy, Department of Pharmacognosy, Makkah, Saudi ArabiaCenter of Research, Faculty of Engineering, Future University in Egypt, New Cairo, EgyptDepartment of Biological Resources, Agricultural Institute, Centre for Agricultural Research, ELKH, 2 Brunszvik St. Martonvásár, Hungary0College of Agronomy, Sichuan Agricultural University, Chengdu, China1Department of Horticultural Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan2Department of Botany, University of Peshawar, Peshawar, PakistanCenter for Plant Sciences and Biodiversity, University of Swat, Charbagh Swat, PakistanBackgroundIncreasing soil salinization has a detrimental effect on agricultural productivity.Therefore, strategies are needed to induce salinity-tolerance in crop species for sustainable foodproduction. γ-aminobutyric acid (GABA) plays a key role in regulating plant salinity stresstolerance. However, it remains largely unknown how mungbean plants (Vigna radiata L.) respondto exogenous GABA under salinity stress.MethodsThus, we evaluated the effect of exogenous GABA (1.5 mM) on the growth and physiobiochemicalresponse mechanism of mungbean plants to saline stress (0-, 50-, and 100 mM [NaCland Na2SO4, at a 1:1 molar ratio]).ResultsIncreased saline stress adversely affected mungbean plants' growth and metabolism. Forinstance, leaf-stem-root biomass (34- and 56%, 31- and 53%, and 27- and 56% under 50- and 100mM, respectively]) and chlorophyll concentrations declined. The carotenoid level increased (10%)at 50 mM and remained unaffected at 100 mM. Hydrogen peroxide (H2O2), malondialdehyde(MDA), osmolytes (soluble sugars, soluble proteins, proline), total phenolic content, andenzymatic activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase(POD), glutathione reductase (GTR), and polyphenol oxidation (PPO) were significantlyincreased. In leaves, salinity caused a significant increase in Na+ concentration but a decrease inK+ concentration, resulting in a low K+/Na+ concentration (51- and 71% under 50- and 100- mMstress). Additionally, nitrogen concentration and the activities of nitrate reductase (NR) andglutamine synthetase (GS) decreased significantly. The reduction in glutamate synthase (GOGAT)activity was only significant (65%) at 100 mM stress. Exogenous GABA decreased Na+, H2O2,and MDA concentrations but enhanced photosynthetic pigments, K+ and K+/Na+ ratio, Nmetabolism, osmolytes, and enzymatic antioxidant activities, thus reducing salinity-associatedstress damages, resulting in improved growth and biomass.ConclusionExogenous GABA may have improved the salinity tolerance of mungbean plants by maintaining their morpho-physiological responses and reducing the accumulation of harmfulsubstances under salinity. Future molecular studies can contribute to a better understanding of themolecular mechanisms by which GABA regulates mungbean salinity tolerance.https://www.frontiersin.org/articles/10.3389/fpls.2022.1081188/fullSalinityplant abiotic stressplant growth regulatorsphytohormonesphysiologicalmechanismantioxidant mechanism |
| spellingShingle | Abd Ullah Abd Ullah Abd Ullah Iftikhar Ali Iftikhar Ali Javaria Noor Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng Sami Bawazeer Sayed M Eldin Muhammad Ahsan Asghar Hafiz Hassan Javed Khansa Saleem Sami Ullah Haider Ali Exogenous γ-aminobutyric acid (GABA) mitigated salinity-induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potential Frontiers in Plant Science Salinity plant abiotic stress plant growth regulators phytohormones physiologicalmechanism antioxidant mechanism |
| title | Exogenous γ-aminobutyric acid (GABA) mitigated salinity-induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potential |
| title_full | Exogenous γ-aminobutyric acid (GABA) mitigated salinity-induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potential |
| title_fullStr | Exogenous γ-aminobutyric acid (GABA) mitigated salinity-induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potential |
| title_full_unstemmed | Exogenous γ-aminobutyric acid (GABA) mitigated salinity-induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potential |
| title_short | Exogenous γ-aminobutyric acid (GABA) mitigated salinity-induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potential |
| title_sort | exogenous γ aminobutyric acid gaba mitigated salinity induced impairments in mungbean plants by regulating their nitrogen metabolism and antioxidant potential |
| topic | Salinity plant abiotic stress plant growth regulators phytohormones physiologicalmechanism antioxidant mechanism |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1081188/full |
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