Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration
Summary: Electrical stimulation has been used to strengthen microbial extracellular electron transfer (EET), however, the deep-seated reasons remain unclear. Here we reported that Bacillus subtilis, a typical gram-positive bacterium capable of extracellular respiration, obtained a higher EET capacit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223001426 |
| _version_ | 1797903342774517760 |
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| author | Qilin Yu Haohao Mao Bowen Yang Yahui Zhu Cheng Sun Zhiqiang Zhao Yang Li Yaobin Zhang |
| author_facet | Qilin Yu Haohao Mao Bowen Yang Yahui Zhu Cheng Sun Zhiqiang Zhao Yang Li Yaobin Zhang |
| author_sort | Qilin Yu |
| collection | DOAJ |
| description | Summary: Electrical stimulation has been used to strengthen microbial extracellular electron transfer (EET), however, the deep-seated reasons remain unclear. Here we reported that Bacillus subtilis, a typical gram-positive bacterium capable of extracellular respiration, obtained a higher EET capacity after the electrical domestication. After the electrical domestication, the current generated by the EET of B. subtilis was 23.4-fold that of the control group without pre-domestication. Multiple lines of evidence in bacterial cells of B. subtilis, their cell walls, and a model tripeptide indicated that the polarization of amide groups after the electrical stimulation forwarded the H-bonds recombination and radical generation of protein-like substances to develop extracellular electron transfer via the proton-coupled pattern. The improved electrochemical properties of protein-like substances benefited the trans-cell-wall electron transfer and strengthen extracellular respiration. This study was the first exploration to promote microbial extracellular respiration by improving the electrochemical properties of protein-like substances in cell envelopes. |
| first_indexed | 2024-04-10T09:31:22Z |
| format | Article |
| id | doaj.art-cd830c4e7187483095ed931af1985d30 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T09:31:22Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-cd830c4e7187483095ed931af1985d302023-02-19T04:27:07ZengElsevieriScience2589-00422023-02-01262106065Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respirationQilin Yu0Haohao Mao1Bowen Yang2Yahui Zhu3Cheng Sun4Zhiqiang Zhao5Yang Li6Yaobin Zhang7Key Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, ChinaKey Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, ChinaKey Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, ChinaKey Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, ChinaKey Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, ChinaKey Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, ChinaSchool of Ocean Science and Technology, Dalian University of Technology, Panjin, Liaoning 124221, ChinaKey Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China; Corresponding authorSummary: Electrical stimulation has been used to strengthen microbial extracellular electron transfer (EET), however, the deep-seated reasons remain unclear. Here we reported that Bacillus subtilis, a typical gram-positive bacterium capable of extracellular respiration, obtained a higher EET capacity after the electrical domestication. After the electrical domestication, the current generated by the EET of B. subtilis was 23.4-fold that of the control group without pre-domestication. Multiple lines of evidence in bacterial cells of B. subtilis, their cell walls, and a model tripeptide indicated that the polarization of amide groups after the electrical stimulation forwarded the H-bonds recombination and radical generation of protein-like substances to develop extracellular electron transfer via the proton-coupled pattern. The improved electrochemical properties of protein-like substances benefited the trans-cell-wall electron transfer and strengthen extracellular respiration. This study was the first exploration to promote microbial extracellular respiration by improving the electrochemical properties of protein-like substances in cell envelopes.http://www.sciencedirect.com/science/article/pii/S2589004223001426ChemistryElectrochemistryBio-electrochemistryBiological sciencesCell biology |
| spellingShingle | Qilin Yu Haohao Mao Bowen Yang Yahui Zhu Cheng Sun Zhiqiang Zhao Yang Li Yaobin Zhang Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration iScience Chemistry Electrochemistry Bio-electrochemistry Biological sciences Cell biology |
| title | Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration |
| title_full | Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration |
| title_fullStr | Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration |
| title_full_unstemmed | Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration |
| title_short | Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration |
| title_sort | electro polarization of protein like substances accelerates trans cell wall electron transfer in microbial extracellular respiration |
| topic | Chemistry Electrochemistry Bio-electrochemistry Biological sciences Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223001426 |
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