Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single Chromosome
Chromosomal inheritance in bacteria usually entails bidirectional replication of a single chromosome from a single origin into two copies and subsequent partitioning of one copy each into daughter cells upon cell division. However, the human pathogen Vibrio cholerae and other Vibrionaceae harbor two...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02932/full |
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| author | Matthias Bruhn Daniel Schindler Franziska S. Kemter Michael R. Wiley Kitty Chase Galina I. Koroleva Gustavo Palacios Shanmuga Sozhamannan Shanmuga Sozhamannan Torsten Waldminghaus |
| author_facet | Matthias Bruhn Daniel Schindler Franziska S. Kemter Michael R. Wiley Kitty Chase Galina I. Koroleva Gustavo Palacios Shanmuga Sozhamannan Shanmuga Sozhamannan Torsten Waldminghaus |
| author_sort | Matthias Bruhn |
| collection | DOAJ |
| description | Chromosomal inheritance in bacteria usually entails bidirectional replication of a single chromosome from a single origin into two copies and subsequent partitioning of one copy each into daughter cells upon cell division. However, the human pathogen Vibrio cholerae and other Vibrionaceae harbor two chromosomes, a large Chr1 and a small Chr2. Chr1 and Chr2 have different origins, an oriC-type origin and a P1 plasmid-type origin, respectively, driving the replication of respective chromosomes. Recently, we described naturally occurring exceptions to the two-chromosome rule of Vibrionaceae: i.e., Chr1 and Chr2 fused single chromosome V. cholerae strains, NSCV1 and NSCV2, in which both origins of replication are present. Using NSCV1 and NSCV2, here we tested whether two types of origins of replication can function simultaneously on the same chromosome or one or the other origin is silenced. We found that in NSCV1, both origins are active whereas in NSCV2 ori2 is silenced despite the fact that it is functional in an isolated context. The ori2 activity appears to be primarily determined by the copy number of the triggering site, crtS, which in turn is determined by its location with respect to ori1 and ori2 on the fused chromosome. |
| first_indexed | 2024-12-12T03:54:18Z |
| format | Article |
| id | doaj.art-cd8706fd30b04017b4beb744cbb5e7f4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T03:54:18Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cd8706fd30b04017b4beb744cbb5e7f42022-12-22T00:39:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-11-01910.3389/fmicb.2018.02932427674Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single ChromosomeMatthias Bruhn0Daniel Schindler1Franziska S. Kemter2Michael R. Wiley3Kitty Chase4Galina I. Koroleva5Gustavo Palacios6Shanmuga Sozhamannan7Shanmuga Sozhamannan8Torsten Waldminghaus9LOEWE Centre for Synthetic Microbiology-SYNMIKRO, Philipps-Universität Marburg, Marburg, GermanyManchester Institute of Biotechnology, The University of Manchester, Manchester, United KingdomLOEWE Centre for Synthetic Microbiology-SYNMIKRO, Philipps-Universität Marburg, Marburg, GermanyUnited States Army Medical Research Institute of Infectious Diseases, Frederick, MD, United StatesUnited States Army Medical Research Institute of Infectious Diseases, Frederick, MD, United StatesUnited States Army Medical Research Institute of Infectious Diseases, Frederick, MD, United StatesUnited States Army Medical Research Institute of Infectious Diseases, Frederick, MD, United StatesDefense Biological Product Assurance Office, Frederick, MD, United StatesThe Tauri Group, LLC, Alexandria, VA, United StatesLOEWE Centre for Synthetic Microbiology-SYNMIKRO, Philipps-Universität Marburg, Marburg, GermanyChromosomal inheritance in bacteria usually entails bidirectional replication of a single chromosome from a single origin into two copies and subsequent partitioning of one copy each into daughter cells upon cell division. However, the human pathogen Vibrio cholerae and other Vibrionaceae harbor two chromosomes, a large Chr1 and a small Chr2. Chr1 and Chr2 have different origins, an oriC-type origin and a P1 plasmid-type origin, respectively, driving the replication of respective chromosomes. Recently, we described naturally occurring exceptions to the two-chromosome rule of Vibrionaceae: i.e., Chr1 and Chr2 fused single chromosome V. cholerae strains, NSCV1 and NSCV2, in which both origins of replication are present. Using NSCV1 and NSCV2, here we tested whether two types of origins of replication can function simultaneously on the same chromosome or one or the other origin is silenced. We found that in NSCV1, both origins are active whereas in NSCV2 ori2 is silenced despite the fact that it is functional in an isolated context. The ori2 activity appears to be primarily determined by the copy number of the triggering site, crtS, which in turn is determined by its location with respect to ori1 and ori2 on the fused chromosome.https://www.frontiersin.org/article/10.3389/fmicb.2018.02932/fullDNA replicationsecondary chromosomeplasmidmultipartite genomereplication initiationpathogens |
| spellingShingle | Matthias Bruhn Daniel Schindler Franziska S. Kemter Michael R. Wiley Kitty Chase Galina I. Koroleva Gustavo Palacios Shanmuga Sozhamannan Shanmuga Sozhamannan Torsten Waldminghaus Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single Chromosome Frontiers in Microbiology DNA replication secondary chromosome plasmid multipartite genome replication initiation pathogens |
| title | Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single Chromosome |
| title_full | Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single Chromosome |
| title_fullStr | Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single Chromosome |
| title_full_unstemmed | Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single Chromosome |
| title_short | Functionality of Two Origins of Replication in Vibrio cholerae Strains With a Single Chromosome |
| title_sort | functionality of two origins of replication in vibrio cholerae strains with a single chromosome |
| topic | DNA replication secondary chromosome plasmid multipartite genome replication initiation pathogens |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02932/full |
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