Changes in the number of circulating CD34+ cells after eccentric exercise of the elbow flexors in relation to muscle damage
Background: It has been reported that strenuous exercise increases the number of bone marrow-derived progenitor cells such as CD34+ cells in the blood, but no previous studies have investigated the changes in circulating CD34+ cells following resistance exercise. This study tested the hypothesis tha...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | Journal of Sport and Health Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209525461400012X |
| _version_ | 1818010741142192128 |
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| author | Ho Seong Lee Makii Muthalib Takayuki Akimoto Kazunori Nosaka |
| author_facet | Ho Seong Lee Makii Muthalib Takayuki Akimoto Kazunori Nosaka |
| author_sort | Ho Seong Lee |
| collection | DOAJ |
| description | Background: It has been reported that strenuous exercise increases the number of bone marrow-derived progenitor cells such as CD34+ cells in the blood, but no previous studies have investigated the changes in circulating CD34+ cells following resistance exercise. This study tested the hypothesis that the number of CD34+ cells in the blood would increase after eccentric exercise of the elbow flexors, but decrease in recovery, and the magnitude of the changes would be dependent on the magnitude of muscle damage.
Methods: Nine men (28.0 ± 6.6 years) performed exercises consisting of 10 sets of six maximal voluntary eccentric contractions of the elbow flexors with their non-dominant arm. Six of them performed the same exercise with the same arm 4 weeks later. Changes in indirect markers of muscle damage were measured before, within 10 min after, and at 24, 48, 72, and 96 h after eccentric exercise. Differential leukocyte counts (total leukocytes, neutrophils, lymphocytes, monocytes) and CD34+ cells in the blood were measured before, immediately after, and at 2, 24, 48, 72, and 96 h following the exercises.
Results: After eccentric exercise, significant (p < 0.05) decreases in maximal voluntary isometric contraction torque and increases in delayed onset muscle soreness and plasma creatine kinase activity were observed. However, no significant changes in leukocytes and CD34+ cells were evident. The changes in muscle damage markers were significantly (p < 0.05) smaller following the second exercise session as compared with the first exercise session, but the changes in leukocytes and CD34+ cells were not significantly different between sessions.
Conclusion: These results did not support the hypothesis, and showed that eccentric exercise-induced muscle damage to the elbow flexors did not influence the number of circulating CD34+ cells. |
| first_indexed | 2024-04-14T05:59:16Z |
| format | Article |
| id | doaj.art-cd8750f9896a4b2fa0be5b001d2741cd |
| institution | Directory Open Access Journal |
| issn | 2095-2546 |
| language | English |
| last_indexed | 2024-04-14T05:59:16Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Sport and Health Science |
| spelling | doaj.art-cd8750f9896a4b2fa0be5b001d2741cd2022-12-22T02:08:50ZengElsevierJournal of Sport and Health Science2095-25462015-09-014327528110.1016/j.jshs.2013.12.005Changes in the number of circulating CD34+ cells after eccentric exercise of the elbow flexors in relation to muscle damageHo Seong Lee0Makii Muthalib1Takayuki Akimoto2Kazunori Nosaka3Department of Kinesiologic Medical Science, Dankook University, Choongnam 330-714, South KoreaInstitute of Health and Biomedical Innovation, School of Human Movement Studies, Queensland University of Technology, Brisbane 4000, AustraliaDivision of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113-8654, JapanSchool of Exercise and Health Sciences, Edith Cowan University, Joondalup, Western Australia 6027, AustraliaBackground: It has been reported that strenuous exercise increases the number of bone marrow-derived progenitor cells such as CD34+ cells in the blood, but no previous studies have investigated the changes in circulating CD34+ cells following resistance exercise. This study tested the hypothesis that the number of CD34+ cells in the blood would increase after eccentric exercise of the elbow flexors, but decrease in recovery, and the magnitude of the changes would be dependent on the magnitude of muscle damage. Methods: Nine men (28.0 ± 6.6 years) performed exercises consisting of 10 sets of six maximal voluntary eccentric contractions of the elbow flexors with their non-dominant arm. Six of them performed the same exercise with the same arm 4 weeks later. Changes in indirect markers of muscle damage were measured before, within 10 min after, and at 24, 48, 72, and 96 h after eccentric exercise. Differential leukocyte counts (total leukocytes, neutrophils, lymphocytes, monocytes) and CD34+ cells in the blood were measured before, immediately after, and at 2, 24, 48, 72, and 96 h following the exercises. Results: After eccentric exercise, significant (p < 0.05) decreases in maximal voluntary isometric contraction torque and increases in delayed onset muscle soreness and plasma creatine kinase activity were observed. However, no significant changes in leukocytes and CD34+ cells were evident. The changes in muscle damage markers were significantly (p < 0.05) smaller following the second exercise session as compared with the first exercise session, but the changes in leukocytes and CD34+ cells were not significantly different between sessions. Conclusion: These results did not support the hypothesis, and showed that eccentric exercise-induced muscle damage to the elbow flexors did not influence the number of circulating CD34+ cells.http://www.sciencedirect.com/science/article/pii/S209525461400012XCreatine kinaseHematopoietic progenitor cellsLengthening contractionsLeukocytesRepeated bout effect |
| spellingShingle | Ho Seong Lee Makii Muthalib Takayuki Akimoto Kazunori Nosaka Changes in the number of circulating CD34+ cells after eccentric exercise of the elbow flexors in relation to muscle damage Journal of Sport and Health Science Creatine kinase Hematopoietic progenitor cells Lengthening contractions Leukocytes Repeated bout effect |
| title | Changes in the number of circulating CD34+ cells after eccentric exercise of the elbow flexors in relation to muscle damage |
| title_full | Changes in the number of circulating CD34+ cells after eccentric exercise of the elbow flexors in relation to muscle damage |
| title_fullStr | Changes in the number of circulating CD34+ cells after eccentric exercise of the elbow flexors in relation to muscle damage |
| title_full_unstemmed | Changes in the number of circulating CD34+ cells after eccentric exercise of the elbow flexors in relation to muscle damage |
| title_short | Changes in the number of circulating CD34+ cells after eccentric exercise of the elbow flexors in relation to muscle damage |
| title_sort | changes in the number of circulating cd34 cells after eccentric exercise of the elbow flexors in relation to muscle damage |
| topic | Creatine kinase Hematopoietic progenitor cells Lengthening contractions Leukocytes Repeated bout effect |
| url | http://www.sciencedirect.com/science/article/pii/S209525461400012X |
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