Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP
Abstract High body mass index (high BMI, obesity) is a serious public health problem, and “obesity‐induced oxidative stress, inflammation, and cancer” have become modern epidemic diseases. We carried out this study to explore a functional beverage that may protect against obesity‐induced diseases. T...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-06-01
|
| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.3335 |
| _version_ | 1797805720644616192 |
|---|---|
| author | Ni Jun Cheng Yi‐Ting Gao Yu‐Ting Zhao Cheng‐Fa Li Li‐Juan She Rong Yang Xiao‐yan Xiao Wen Yang Xu |
| author_facet | Ni Jun Cheng Yi‐Ting Gao Yu‐Ting Zhao Cheng‐Fa Li Li‐Juan She Rong Yang Xiao‐yan Xiao Wen Yang Xu |
| author_sort | Ni Jun |
| collection | DOAJ |
| description | Abstract High body mass index (high BMI, obesity) is a serious public health problem, and “obesity‐induced oxidative stress, inflammation, and cancer” have become modern epidemic diseases. We carried out this study to explore a functional beverage that may protect against obesity‐induced diseases. The Engleromyces goetzei Henn herbal tea is such a candidate. For this study, we carried out LC–MS analysis of E. goetzei Henn aqueous extract (EgH‐AE); then used the Caco‐2 cell line for the model cells and treated the cells with t‐BHP to form an oxidative stress system. An MTT assay was used for testing the biocompatibility and cytoprotective effects; reactive oxygen species and malondialdehyde determination was used for evaluating the antioxidative stress effect; TNF‐α and IL‐1β were used for observing the anti‐inflammatory effect, and 8‐OHdG for monitoring anticancer activity. The results of this study demonstrate that the EgH‐AE has very good biocompatibility with the Caco‐2 cell line and has good cytoprotective, antioxidant, anti‐inflammatory, and anticancer properties. It is clear that EgH‐AE, a kind of ancient herbal tea, may be used to develop a functional beverage that can be given to people with a high BMI to protect against obesity‐induced diseases. |
| first_indexed | 2024-03-13T05:56:20Z |
| format | Article |
| id | doaj.art-cd87b26b363b4377a9472a5e5d210e38 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-13T05:56:20Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-cd87b26b363b4377a9472a5e5d210e382023-06-13T05:10:23ZengWileyFood Science & Nutrition2048-71772023-06-011163450346310.1002/fsn3.3335Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHPNi Jun0Cheng Yi‐Ting1Gao Yu‐Ting2Zhao Cheng‐Fa3Li Li‐Juan4She Rong5Yang Xiao‐yan6Xiao Wen7Yang Xu8Institute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaInstitute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaInstitute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaInstitute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaInstitute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaInstitute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaInstitute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaInstitute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaInstitute of Natural Antioxidants and Antioxidant Inflammation Dali University Dali 671003 ChinaAbstract High body mass index (high BMI, obesity) is a serious public health problem, and “obesity‐induced oxidative stress, inflammation, and cancer” have become modern epidemic diseases. We carried out this study to explore a functional beverage that may protect against obesity‐induced diseases. The Engleromyces goetzei Henn herbal tea is such a candidate. For this study, we carried out LC–MS analysis of E. goetzei Henn aqueous extract (EgH‐AE); then used the Caco‐2 cell line for the model cells and treated the cells with t‐BHP to form an oxidative stress system. An MTT assay was used for testing the biocompatibility and cytoprotective effects; reactive oxygen species and malondialdehyde determination was used for evaluating the antioxidative stress effect; TNF‐α and IL‐1β were used for observing the anti‐inflammatory effect, and 8‐OHdG for monitoring anticancer activity. The results of this study demonstrate that the EgH‐AE has very good biocompatibility with the Caco‐2 cell line and has good cytoprotective, antioxidant, anti‐inflammatory, and anticancer properties. It is clear that EgH‐AE, a kind of ancient herbal tea, may be used to develop a functional beverage that can be given to people with a high BMI to protect against obesity‐induced diseases.https://doi.org/10.1002/fsn3.3335anticanceranti‐inflammatoryantioxidative stressbiocompatibilityEngleromyces goetzei Henn (EgH)LC–MS analysis |
| spellingShingle | Ni Jun Cheng Yi‐Ting Gao Yu‐Ting Zhao Cheng‐Fa Li Li‐Juan She Rong Yang Xiao‐yan Xiao Wen Yang Xu Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP Food Science & Nutrition anticancer anti‐inflammatory antioxidative stress biocompatibility Engleromyces goetzei Henn (EgH) LC–MS analysis |
| title | Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP |
| title_full | Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP |
| title_fullStr | Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP |
| title_full_unstemmed | Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP |
| title_short | Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP |
| title_sort | antioxidant anti inflammatory and anticancer function of engleromyces goetzei henn aqueous extract on human intestinal caco 2 cells treated with t bhp |
| topic | anticancer anti‐inflammatory antioxidative stress biocompatibility Engleromyces goetzei Henn (EgH) LC–MS analysis |
| url | https://doi.org/10.1002/fsn3.3335 |
| work_keys_str_mv | AT nijun antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp AT chengyiting antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp AT gaoyuting antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp AT zhaochengfa antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp AT lilijuan antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp AT sherong antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp AT yangxiaoyan antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp AT xiaowen antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp AT yangxu antioxidantantiinflammatoryandanticancerfunctionofengleromycesgoetzeihennaqueousextractonhumanintestinalcaco2cellstreatedwithtbhp |