Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME)
Prebiotic compounds aim at improving human health by targeting beneficial gut microbes. We used a dynamic in vitro model of the human digestive tract (M-SHIME®) to study the modulatory effects of long-chain arabinoxylans (LC-AX) towards luminal and mucosal microbiota. Two doses (3 and 6 g/L) of LC-A...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-05-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617300762 |
| _version_ | 1818617682185945088 |
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| author | Pilar Truchado Emma Hernandez-Sanabria Bouke N. Salden Pieter Van den Abbeele Ramiro Vilchez-Vargas Ruy Jauregui Dietmar H. Pieper Sam Possemiers Tom Van de Wiele |
| author_facet | Pilar Truchado Emma Hernandez-Sanabria Bouke N. Salden Pieter Van den Abbeele Ramiro Vilchez-Vargas Ruy Jauregui Dietmar H. Pieper Sam Possemiers Tom Van de Wiele |
| author_sort | Pilar Truchado |
| collection | DOAJ |
| description | Prebiotic compounds aim at improving human health by targeting beneficial gut microbes. We used a dynamic in vitro model of the human digestive tract (M-SHIME®) to study the modulatory effects of long-chain arabinoxylans (LC-AX) towards luminal and mucosal microbiota. Two doses (3 and 6 g/L) of LC-AX were supplied to the proximal colon compartments of the M-SHIME, three times per day for 3 days. Next generation sequencing and multivariate statistical analysis revealed the distribution of key microbial genera across M-SHIME compartments. Supplementation of 6 g/L of LC-AX significantly increased the relative abundance of Bifidobacterium in both lumen (from 0.34% to 0.41%, 1.2-fold) and mucus (from 0.14% to 0.29%, 2.1-fold) compared with the control (P < 0.001). Propionate (1.4-fold), as well as activity of β-xylanase, β-xylosidase and α-arabinofuranosidase (P < 0.05) were also increased in the lumen. LC-AX may potentially benefit host health by stimulating Bifidobacterium abundance and its metabolic activity. |
| first_indexed | 2024-12-16T17:09:34Z |
| format | Article |
| id | doaj.art-cd8ab6185fc84c699437532f9834abf9 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T17:09:34Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-cd8ab6185fc84c699437532f9834abf92022-12-21T22:23:28ZengElsevierJournal of Functional Foods1756-46462017-05-0132226237Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME)Pilar Truchado0Emma Hernandez-Sanabria1Bouke N. Salden2Pieter Van den Abbeele3Ramiro Vilchez-Vargas4Ruy Jauregui5Dietmar H. Pieper6Sam Possemiers7Tom Van de Wiele8Centre for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, BelgiumCentre for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, BelgiumDepartment of Internal Medicine, Division of Gastroenterology-Hepatology, NUTRIM, Maastricht University Medical Centre, Maastricht, NetherlandsCentre for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, BelgiumCentre for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, BelgiumMicrobial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstraβe 7, 38124 Braunschweig, GermanyMicrobial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstraβe 7, 38124 Braunschweig, GermanyCentre for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, BelgiumCentre for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, Belgium; Corresponding author at: CMET – UGent, Coupure Links 653, B-9000 Ghent, Belgium.Prebiotic compounds aim at improving human health by targeting beneficial gut microbes. We used a dynamic in vitro model of the human digestive tract (M-SHIME®) to study the modulatory effects of long-chain arabinoxylans (LC-AX) towards luminal and mucosal microbiota. Two doses (3 and 6 g/L) of LC-AX were supplied to the proximal colon compartments of the M-SHIME, three times per day for 3 days. Next generation sequencing and multivariate statistical analysis revealed the distribution of key microbial genera across M-SHIME compartments. Supplementation of 6 g/L of LC-AX significantly increased the relative abundance of Bifidobacterium in both lumen (from 0.34% to 0.41%, 1.2-fold) and mucus (from 0.14% to 0.29%, 2.1-fold) compared with the control (P < 0.001). Propionate (1.4-fold), as well as activity of β-xylanase, β-xylosidase and α-arabinofuranosidase (P < 0.05) were also increased in the lumen. LC-AX may potentially benefit host health by stimulating Bifidobacterium abundance and its metabolic activity.http://www.sciencedirect.com/science/article/pii/S1756464617300762PrebioticsMucusBifidobacteriumMicrobiomePropionateIn vitro |
| spellingShingle | Pilar Truchado Emma Hernandez-Sanabria Bouke N. Salden Pieter Van den Abbeele Ramiro Vilchez-Vargas Ruy Jauregui Dietmar H. Pieper Sam Possemiers Tom Van de Wiele Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME) Journal of Functional Foods Prebiotics Mucus Bifidobacterium Microbiome Propionate In vitro |
| title | Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME) |
| title_full | Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME) |
| title_fullStr | Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME) |
| title_full_unstemmed | Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME) |
| title_short | Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME) |
| title_sort | long chain arabinoxylans shift the mucosa associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem m shime |
| topic | Prebiotics Mucus Bifidobacterium Microbiome Propionate In vitro |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617300762 |
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