Suppression of MiR130a-3p Using CRISPR/Cas9 Induces Proliferation and Migration of Non-Small Cell Lung Cancer Cell Line
BACKGROUND: Molecular alterations of microRNA130a (miR130a) are observed in many types of cancers, including non-small cell lung cancer (NSCLC). However, the role of miR130a in NSCLC has been poorly studied. METHODS: In this study, clustered regularly interspaced short palindromic repeat (CRISPR)-C...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Secretariat of The Indonesian Biomedical Journal
2021-12-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/1670 |
| _version_ | 1819098380374114304 |
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| author | Nur Ainina Abdollah Nabil Izzatie Mohamad Safiai Muhammad Khairi Ahmad Kumitaa Theva Das Siti Razila Abdul Razak |
| author_facet | Nur Ainina Abdollah Nabil Izzatie Mohamad Safiai Muhammad Khairi Ahmad Kumitaa Theva Das Siti Razila Abdul Razak |
| author_sort | Nur Ainina Abdollah |
| collection | DOAJ |
| description | BACKGROUND: Molecular alterations of microRNA130a (miR130a) are observed in many types of cancers, including non-small cell lung cancer (NSCLC). However, the role of miR130a in NSCLC has been poorly studied.
METHODS: In this study, clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 was utilised to knockdown miR130a. The gRNA was designed to target the stem loop, 3’ and 5’ sites of miR130a and stably expressed in A549 cells. Post-treatment, mature levels of miR130a-3p and 5p were quantified, and proliferation and migration assays were conducted.
RESULTS: Result showed significant suppression of miR130a-3p and -5p by two and three-fold respectively, when the CRISPR/Cas9 targeted at the 3’ site and stem loop of the miR130a gene. Suppression of miR130a-3p significantly increased the growth and migration of A549 cells, but no significant changes were observed in cells with suppressed expression of miR130a-5p.
CONCLUSION: Our encouraging results highlight that the suppression of the miR130a is achievable using CRISPR/Cas9, and suppression of the miR-130a-3p could play an important role in the regulation of NSCLC.
KEYWORDS: miR130a, CRISPR-Cas9, non-small cell lung cancer |
| first_indexed | 2024-12-22T00:30:04Z |
| format | Article |
| id | doaj.art-cd8f6b781b6f423fa0c22d3d5a5abf7e |
| institution | Directory Open Access Journal |
| issn | 2085-3297 2355-9179 |
| language | English |
| last_indexed | 2024-12-22T00:30:04Z |
| publishDate | 2021-12-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj.art-cd8f6b781b6f423fa0c22d3d5a5abf7e2022-12-21T18:44:57ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792021-12-011343647410.18585/inabj.v13i4.1670430Suppression of MiR130a-3p Using CRISPR/Cas9 Induces Proliferation and Migration of Non-Small Cell Lung Cancer Cell LineNur Ainina Abdollah0Nabil Izzatie Mohamad Safiai1Muhammad Khairi Ahmad2Kumitaa Theva Das3Siti Razila Abdul Razak4Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Kepala Batas, Pulau Pinang, 13200Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Kepala Batas, Pulau Pinang, 13200Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Kepala Batas, Pulau Pinang, 13200Infectomics Cluster, Advanced Medical and Dental Institute, Kepala Batas, Pulau Pinang, 13200Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Kepala Batas, Pulau Pinang, 13200BACKGROUND: Molecular alterations of microRNA130a (miR130a) are observed in many types of cancers, including non-small cell lung cancer (NSCLC). However, the role of miR130a in NSCLC has been poorly studied. METHODS: In this study, clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 was utilised to knockdown miR130a. The gRNA was designed to target the stem loop, 3’ and 5’ sites of miR130a and stably expressed in A549 cells. Post-treatment, mature levels of miR130a-3p and 5p were quantified, and proliferation and migration assays were conducted. RESULTS: Result showed significant suppression of miR130a-3p and -5p by two and three-fold respectively, when the CRISPR/Cas9 targeted at the 3’ site and stem loop of the miR130a gene. Suppression of miR130a-3p significantly increased the growth and migration of A549 cells, but no significant changes were observed in cells with suppressed expression of miR130a-5p. CONCLUSION: Our encouraging results highlight that the suppression of the miR130a is achievable using CRISPR/Cas9, and suppression of the miR-130a-3p could play an important role in the regulation of NSCLC. KEYWORDS: miR130a, CRISPR-Cas9, non-small cell lung cancerhttps://inabj.org/index.php/ibj/article/view/1670 |
| spellingShingle | Nur Ainina Abdollah Nabil Izzatie Mohamad Safiai Muhammad Khairi Ahmad Kumitaa Theva Das Siti Razila Abdul Razak Suppression of MiR130a-3p Using CRISPR/Cas9 Induces Proliferation and Migration of Non-Small Cell Lung Cancer Cell Line Indonesian Biomedical Journal |
| title | Suppression of MiR130a-3p Using CRISPR/Cas9 Induces Proliferation and Migration of Non-Small Cell Lung Cancer Cell Line |
| title_full | Suppression of MiR130a-3p Using CRISPR/Cas9 Induces Proliferation and Migration of Non-Small Cell Lung Cancer Cell Line |
| title_fullStr | Suppression of MiR130a-3p Using CRISPR/Cas9 Induces Proliferation and Migration of Non-Small Cell Lung Cancer Cell Line |
| title_full_unstemmed | Suppression of MiR130a-3p Using CRISPR/Cas9 Induces Proliferation and Migration of Non-Small Cell Lung Cancer Cell Line |
| title_short | Suppression of MiR130a-3p Using CRISPR/Cas9 Induces Proliferation and Migration of Non-Small Cell Lung Cancer Cell Line |
| title_sort | suppression of mir130a 3p using crispr cas9 induces proliferation and migration of non small cell lung cancer cell line |
| url | https://inabj.org/index.php/ibj/article/view/1670 |
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