Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar Rats
Hypertension is a condition of persistently high blood pressure. It is currently a big health issue as its prevalence is high in Indonesia and its complications are numerous and deadly. Salt intake is one of the modifiable factors of hypertension. According to a study by Indonesian Ministry of Healt...
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| Format: | Article |
| Language: | English |
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Universitas Padjajaran
2019-12-01
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| Series: | Majalah Kedokteran Bandung |
| Subjects: | |
| Online Access: | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1764 |
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| author | Ronny Lesmana Genta Syaifrin Laudza Trianing Tyas Kusuma Hanna Goenawan Yulia Sofiatin Yuni Susanti Pratiwi Nova Sylviana Setiawan Setiawan |
| author_facet | Ronny Lesmana Genta Syaifrin Laudza Trianing Tyas Kusuma Hanna Goenawan Yulia Sofiatin Yuni Susanti Pratiwi Nova Sylviana Setiawan Setiawan |
| author_sort | Ronny Lesmana |
| collection | DOAJ |
| description | Hypertension is a condition of persistently high blood pressure. It is currently a big health issue as its prevalence is high in Indonesia and its complications are numerous and deadly. Salt intake is one of the modifiable factors of hypertension. According to a study by Indonesian Ministry of Health, salt consumption in Indonesia is almost two times greater than the recommended salt diet from WHO. Sodium reabsorption in kidney plays a role in regulating blood pressure. Epithelial sodium channel (ENaC) is one of the structures that function in sodium reabsorption in kidney tubules. This study was conducted at the Central Laboratory and Animal Laboratory of the Faculty of Medicine Universitas Padjadjaran from June to Desember 2018. The aim of this study was to analyze the effect of high sodium diet on the expression of ENaC gene in kidney tubules of rats. Twelve Rattus norvegicus wistar rats were divided into two groups of control and treatment. Treatment group was given daily 2 mL NaCl solution treatment using gavage for 8 weeks. The expression of ENaC α, β, and γ was obtained by running tissue samples from kidney cortex and medulla in polymerase chain reaction and electrophoresis. The result showed that there was an insignificant decrease in ENaC α, β, and γ gene expressions in both kidney cortex and medulla of the treatment group when compared to control groupThis study concludes that ENaC gene expression is not significantly affected by high sodium diet. |
| first_indexed | 2024-12-11T05:02:30Z |
| format | Article |
| id | doaj.art-cd917c0cca254766b11278e59ee73568 |
| institution | Directory Open Access Journal |
| issn | 0126-074X 2338-6223 |
| language | English |
| last_indexed | 2024-12-11T05:02:30Z |
| publishDate | 2019-12-01 |
| publisher | Universitas Padjajaran |
| record_format | Article |
| series | Majalah Kedokteran Bandung |
| spelling | doaj.art-cd917c0cca254766b11278e59ee735682022-12-22T01:20:07ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232019-12-0151424024510.15395/mkb.v51n4.17641138Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar RatsRonny Lesmana0Genta Syaifrin Laudza1Trianing Tyas Kusuma2Hanna Goenawan3Yulia Sofiatin4Yuni Susanti Pratiwi5Nova Sylviana6Setiawan Setiawan7Physiology Division, Departement of Biomedical Sciences Faculty of Medicine Universitas PadjadjaranFaculty of Medicine Universitas Padjadjaran, IndonesiaPhysiology Molecular Laboratory, Biological Activity Division, Central Laboratory, Universitas Padjadjaran, BandungPhysiology Division, Departement of Biomedical Sciences Faculty of Medicine Universitas PadjadjaranDepartment of Public Health Faculty of Medicine Universitas Padjadjaran BandungPhysiology Division, Departement of Biomedical Sciences Faculty of Medicine Universitas PadjadjaranPhysiology Division, Departement of Biomedical Sciences Faculty of Medicine Universitas PadjadjaranPhysiology Division, Departement of Biomedical Sciences Faculty of Medicine Universitas PadjadjaranHypertension is a condition of persistently high blood pressure. It is currently a big health issue as its prevalence is high in Indonesia and its complications are numerous and deadly. Salt intake is one of the modifiable factors of hypertension. According to a study by Indonesian Ministry of Health, salt consumption in Indonesia is almost two times greater than the recommended salt diet from WHO. Sodium reabsorption in kidney plays a role in regulating blood pressure. Epithelial sodium channel (ENaC) is one of the structures that function in sodium reabsorption in kidney tubules. This study was conducted at the Central Laboratory and Animal Laboratory of the Faculty of Medicine Universitas Padjadjaran from June to Desember 2018. The aim of this study was to analyze the effect of high sodium diet on the expression of ENaC gene in kidney tubules of rats. Twelve Rattus norvegicus wistar rats were divided into two groups of control and treatment. Treatment group was given daily 2 mL NaCl solution treatment using gavage for 8 weeks. The expression of ENaC α, β, and γ was obtained by running tissue samples from kidney cortex and medulla in polymerase chain reaction and electrophoresis. The result showed that there was an insignificant decrease in ENaC α, β, and γ gene expressions in both kidney cortex and medulla of the treatment group when compared to control groupThis study concludes that ENaC gene expression is not significantly affected by high sodium diet.http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1764cortex, dietary sodium, α enac, β enac, γ enac, kidney, medulla |
| spellingShingle | Ronny Lesmana Genta Syaifrin Laudza Trianing Tyas Kusuma Hanna Goenawan Yulia Sofiatin Yuni Susanti Pratiwi Nova Sylviana Setiawan Setiawan Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar Rats Majalah Kedokteran Bandung cortex, dietary sodium, α enac, β enac, γ enac, kidney, medulla |
| title | Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar Rats |
| title_full | Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar Rats |
| title_fullStr | Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar Rats |
| title_full_unstemmed | Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar Rats |
| title_short | Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar Rats |
| title_sort | effect of dietary sodium on α β and γ epithelial sodium channel enac gene expression in kidney tubules of wistar rats |
| topic | cortex, dietary sodium, α enac, β enac, γ enac, kidney, medulla |
| url | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1764 |
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