Laboratory Culture and Life Cycle of <i>Thelazia callipaeda</i> in Intermediate and Definitive Hosts
Human thelaziasis caused by <i>Thelazia callipaeda</i> is being increasingly reported worldwide. Notably, an epidemic trend is observed in Southwest China. Whether <i>Phortica okadai</i> found in Southwest China can act as a vector of <i>T. callipaeda</i> and huma...
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MDPI AG
2022-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/9/1066 |
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| author | Lingjun Wang Di Li Changzhu Yin Hongri Tang Bo Luo Rong Yan Yujuan Shen Hui Liu |
| author_facet | Lingjun Wang Di Li Changzhu Yin Hongri Tang Bo Luo Rong Yan Yujuan Shen Hui Liu |
| author_sort | Lingjun Wang |
| collection | DOAJ |
| description | Human thelaziasis caused by <i>Thelazia callipaeda</i> is being increasingly reported worldwide. Notably, an epidemic trend is observed in Southwest China. Whether <i>Phortica okadai</i> found in Southwest China can act as a vector of <i>T. callipaeda</i> and human-derived <i>T. callipaeda</i> animal infections has not been widely reported. Here, <i>P</i>. <i>okadai</i> was maintained in a laboratory and experimentally infected with first-stage larvae collected from adult <i>T. callipaeda</i> that were isolated from infected human subjects. Dead <i>P. okadai</i> were subjected to PCR assay and dissected every two days to detect <i>T. callipaeda</i>. Subsequently, live flies were used to infect a rabbit. The infection procedures were performed once a day (20 min) for two weeks. The results show that L1 collected from the adult <i>T. callipaeda</i> could successfully parasitize <i>P. okadai</i> captured in Zunyi, a city in Southwest China, and developed into L3, and a rabbit was successfully infected with <i>T. callipaeda</i> using <i>P. okadai</i> as the intermediate host. The present study demonstrates a human-derived <i>T. callipaeda</i> infection in rabbits, through <i>P. okadai</i>, under laboratory conditions for the first time. These results provide insights into the transmission cycle of <i>T. callipaeda</i> and constitute a foundation to develop an effective treatment protocol for <i>T. callipaeda</i> infection. |
| first_indexed | 2024-03-09T22:52:39Z |
| format | Article |
| id | doaj.art-cd943ea9cd584538bdaad77620a32a3c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:52:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-cd943ea9cd584538bdaad77620a32a3c2023-11-23T18:16:52ZengMDPI AGPathogens2076-08172022-09-01119106610.3390/pathogens11091066Laboratory Culture and Life Cycle of <i>Thelazia callipaeda</i> in Intermediate and Definitive HostsLingjun Wang0Di Li1Changzhu Yin2Hongri Tang3Bo Luo4Rong Yan5Yujuan Shen6Hui Liu7Department of Parasitology, Zunyi Medical University, Zunyi 563000, ChinaDepartment of Parasitology, Zunyi Medical University, Zunyi 563000, ChinaDepartment of Parasitology, Zunyi Medical University, Zunyi 563000, ChinaDepartment of Parasitology, Zunyi Medical University, Zunyi 563000, ChinaDepartment of Parasitology, Zunyi Medical University, Zunyi 563000, ChinaDepartment of Parasitology, Zunyi Medical University, Zunyi 563000, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, ChinaDepartment of Parasitology, Zunyi Medical University, Zunyi 563000, ChinaHuman thelaziasis caused by <i>Thelazia callipaeda</i> is being increasingly reported worldwide. Notably, an epidemic trend is observed in Southwest China. Whether <i>Phortica okadai</i> found in Southwest China can act as a vector of <i>T. callipaeda</i> and human-derived <i>T. callipaeda</i> animal infections has not been widely reported. Here, <i>P</i>. <i>okadai</i> was maintained in a laboratory and experimentally infected with first-stage larvae collected from adult <i>T. callipaeda</i> that were isolated from infected human subjects. Dead <i>P. okadai</i> were subjected to PCR assay and dissected every two days to detect <i>T. callipaeda</i>. Subsequently, live flies were used to infect a rabbit. The infection procedures were performed once a day (20 min) for two weeks. The results show that L1 collected from the adult <i>T. callipaeda</i> could successfully parasitize <i>P. okadai</i> captured in Zunyi, a city in Southwest China, and developed into L3, and a rabbit was successfully infected with <i>T. callipaeda</i> using <i>P. okadai</i> as the intermediate host. The present study demonstrates a human-derived <i>T. callipaeda</i> infection in rabbits, through <i>P. okadai</i>, under laboratory conditions for the first time. These results provide insights into the transmission cycle of <i>T. callipaeda</i> and constitute a foundation to develop an effective treatment protocol for <i>T. callipaeda</i> infection.https://www.mdpi.com/2076-0817/11/9/1066<i>Thelazia callipaeda</i><i>Phortica okadai</i>vector-borne zoonosisanimal modelintermediate host |
| spellingShingle | Lingjun Wang Di Li Changzhu Yin Hongri Tang Bo Luo Rong Yan Yujuan Shen Hui Liu Laboratory Culture and Life Cycle of <i>Thelazia callipaeda</i> in Intermediate and Definitive Hosts Pathogens <i>Thelazia callipaeda</i> <i>Phortica okadai</i> vector-borne zoonosis animal model intermediate host |
| title | Laboratory Culture and Life Cycle of <i>Thelazia callipaeda</i> in Intermediate and Definitive Hosts |
| title_full | Laboratory Culture and Life Cycle of <i>Thelazia callipaeda</i> in Intermediate and Definitive Hosts |
| title_fullStr | Laboratory Culture and Life Cycle of <i>Thelazia callipaeda</i> in Intermediate and Definitive Hosts |
| title_full_unstemmed | Laboratory Culture and Life Cycle of <i>Thelazia callipaeda</i> in Intermediate and Definitive Hosts |
| title_short | Laboratory Culture and Life Cycle of <i>Thelazia callipaeda</i> in Intermediate and Definitive Hosts |
| title_sort | laboratory culture and life cycle of i thelazia callipaeda i in intermediate and definitive hosts |
| topic | <i>Thelazia callipaeda</i> <i>Phortica okadai</i> vector-borne zoonosis animal model intermediate host |
| url | https://www.mdpi.com/2076-0817/11/9/1066 |
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