Genome-Wide Identification and Expression Analysis of Wall-Associated Kinase (WAK) Gene Family in <i>Cannabis sativa</i> L.
Wall-associated kinases (WAKs) are receptors that bind pectin or small pectic fragments in the cell wall and play roles in cell elongation and pathogen response. In the <i>Cannabis sativa</i> (Cs) genome, 53 CsWAK/CsWAKL (WAK-like) protein family members were identified and characterized...
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/20/2703 |
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| author | Hülya Sipahi Terik Djabeng Whyte Gang Ma Gerald Berkowitz |
| author_facet | Hülya Sipahi Terik Djabeng Whyte Gang Ma Gerald Berkowitz |
| author_sort | Hülya Sipahi |
| collection | DOAJ |
| description | Wall-associated kinases (WAKs) are receptors that bind pectin or small pectic fragments in the cell wall and play roles in cell elongation and pathogen response. In the <i>Cannabis sativa</i> (Cs) genome, 53 CsWAK/CsWAKL (WAK-like) protein family members were identified and characterized; their amino acid lengths and molecular weights varied from 582 to 983, and from 65.6 to 108.8 kDa, respectively. They were classified into four main groups by a phylogenetic tree. Out of the 53 identified <i>CsWAK/CsWAKL</i> genes, 23 <i>CsWAK/CsWAKL</i> genes were unevenly distributed among six chromosomes. Two pairs of genes on chromosomes 4 and 7 have undergone duplication. The number of introns and exons among <i>CsWAK/CsWAKL</i> genes ranged from 1 to 6 and from 2 to 7, respectively. The promoter regions of 23 <i>CsWAKs</i>/<i>CsWAKLs</i> possessed diverse cis-regulatory elements that are involved in light, development, environmental stress, and hormone responsiveness. The expression profiles indicated that our candidate genes (<i>CsWAK1, CsWAK4, CsWAK7, CsWAKL1, and CsWAKL7</i>) are expressed in leaf tissue. These genes exhibit different expression patterns than their homologs in other plant species. These initial findings are useful resources for further research work on the potential roles of CsWAK/CsWAKL in cellular signalling during development, environmental stress conditions, and hormone treatments. |
| first_indexed | 2024-03-09T19:34:00Z |
| format | Article |
| id | doaj.art-cd9a7ad2b9bb4f2abdc580b0e1d20688 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T19:34:00Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-cd9a7ad2b9bb4f2abdc580b0e1d206882023-11-24T02:03:52ZengMDPI AGPlants2223-77472022-10-011120270310.3390/plants11202703Genome-Wide Identification and Expression Analysis of Wall-Associated Kinase (WAK) Gene Family in <i>Cannabis sativa</i> L.Hülya Sipahi0Terik Djabeng Whyte1Gang Ma2Gerald Berkowitz3Department of Agricultural Biotechnology, Faculty of Agriculture, University of Eskişehir Osmangazi, Eskişehir 26160, TürkiyeDepartment of Agricultural Biotechnology, Faculty of Agriculture, University of Eskişehir Osmangazi, Eskişehir 26160, TürkiyeAgricultural Biotechnology Laboratory, Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USAAgricultural Biotechnology Laboratory, Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USAWall-associated kinases (WAKs) are receptors that bind pectin or small pectic fragments in the cell wall and play roles in cell elongation and pathogen response. In the <i>Cannabis sativa</i> (Cs) genome, 53 CsWAK/CsWAKL (WAK-like) protein family members were identified and characterized; their amino acid lengths and molecular weights varied from 582 to 983, and from 65.6 to 108.8 kDa, respectively. They were classified into four main groups by a phylogenetic tree. Out of the 53 identified <i>CsWAK/CsWAKL</i> genes, 23 <i>CsWAK/CsWAKL</i> genes were unevenly distributed among six chromosomes. Two pairs of genes on chromosomes 4 and 7 have undergone duplication. The number of introns and exons among <i>CsWAK/CsWAKL</i> genes ranged from 1 to 6 and from 2 to 7, respectively. The promoter regions of 23 <i>CsWAKs</i>/<i>CsWAKLs</i> possessed diverse cis-regulatory elements that are involved in light, development, environmental stress, and hormone responsiveness. The expression profiles indicated that our candidate genes (<i>CsWAK1, CsWAK4, CsWAK7, CsWAKL1, and CsWAKL7</i>) are expressed in leaf tissue. These genes exhibit different expression patterns than their homologs in other plant species. These initial findings are useful resources for further research work on the potential roles of CsWAK/CsWAKL in cellular signalling during development, environmental stress conditions, and hormone treatments.https://www.mdpi.com/2223-7747/11/20/2703<i>Cannabis sativa</i> L.wall-associated kinasegene expression |
| spellingShingle | Hülya Sipahi Terik Djabeng Whyte Gang Ma Gerald Berkowitz Genome-Wide Identification and Expression Analysis of Wall-Associated Kinase (WAK) Gene Family in <i>Cannabis sativa</i> L. Plants <i>Cannabis sativa</i> L. wall-associated kinase gene expression |
| title | Genome-Wide Identification and Expression Analysis of Wall-Associated Kinase (WAK) Gene Family in <i>Cannabis sativa</i> L. |
| title_full | Genome-Wide Identification and Expression Analysis of Wall-Associated Kinase (WAK) Gene Family in <i>Cannabis sativa</i> L. |
| title_fullStr | Genome-Wide Identification and Expression Analysis of Wall-Associated Kinase (WAK) Gene Family in <i>Cannabis sativa</i> L. |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of Wall-Associated Kinase (WAK) Gene Family in <i>Cannabis sativa</i> L. |
| title_short | Genome-Wide Identification and Expression Analysis of Wall-Associated Kinase (WAK) Gene Family in <i>Cannabis sativa</i> L. |
| title_sort | genome wide identification and expression analysis of wall associated kinase wak gene family in i cannabis sativa i l |
| topic | <i>Cannabis sativa</i> L. wall-associated kinase gene expression |
| url | https://www.mdpi.com/2223-7747/11/20/2703 |
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