Green synthesis of Kickxia elatine-induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of Alzheimer’s disease
The synthesis of silver nanoparticles (AgNPs) by the green method is favored as compared to chemical synthesis due to their appreciable properties of less toxicity and simple synthesis. The current study designed the biosynthesis of AgNPs in one step by using the plant Kickxia elatine (KE) extract a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2023-06-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2023-0060 |
| _version_ | 1827928228674666496 |
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| author | Huda Noor Ul Ghneim Hazem K. Fozia Fozia Ahmed Mushtaq Mushtaq Nadia Sher Naila Khan Rahmat Ali Ahmad Ijaz Al-Sheikh Yazeed A. Giesy John P. Aboul-Soud Mourad A. M. |
| author_facet | Huda Noor Ul Ghneim Hazem K. Fozia Fozia Ahmed Mushtaq Mushtaq Nadia Sher Naila Khan Rahmat Ali Ahmad Ijaz Al-Sheikh Yazeed A. Giesy John P. Aboul-Soud Mourad A. M. |
| author_sort | Huda Noor Ul |
| collection | DOAJ |
| description | The synthesis of silver nanoparticles (AgNPs) by the green method is favored as compared to chemical synthesis due to their appreciable properties of less toxicity and simple synthesis. The current study designed the biosynthesis of AgNPs in one step by using the plant Kickxia elatine (KE) extract and then investigated its inhibiting activity against rat’s brain acetylcholinesterase (AChE) ex vivo. Ultraviolet spectrum at 416 nm confirmed the formation of AgNPs. X-ray diffractometer calculated size was reported to be 42.47 nm. The SEM analysis confirmed spherical-shaped AgNPs. FT-IR suggested that the phytochemical groups present in the KE extract and their nanoparticles (NPs) are responsible for the biosynthesized of NPs. EDX analysis presented that Ag was the chief element with 61.67%. Both KE extract and AgNPs showed significant anti-AChE activity at 175 µg·mL−1. Statistical analysis showed that both KE and AgNPs exhibited non-competitive type inhibition against AChE, i.e. V
max decreased (34.17–68.64% and 22.29–62.10%), while K
m values remained constant. It is concluded that KE and AgNPs can be considered an inhibitor of rats’ brain AChE. Furthermore, the synthesis of AgNP-based drugs can be used as a cheaper and alternative option against diseases such as Alzheimer’s disease. |
| first_indexed | 2024-03-13T06:04:41Z |
| format | Article |
| id | doaj.art-cd9b3c86d33d46748920e2e673d66740 |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-03-13T06:04:41Z |
| publishDate | 2023-06-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-cd9b3c86d33d46748920e2e673d667402023-06-12T06:30:52ZengDe GruyterGreen Processing and Synthesis2191-95502023-06-01121e69502310.1515/gps-2023-0060Green synthesis of Kickxia elatine-induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of Alzheimer’s diseaseHuda Noor Ul0Ghneim Hazem K.1Fozia Fozia2Ahmed Mushtaq3Mushtaq Nadia4Sher Naila5Khan Rahmat Ali6Ahmad Ijaz7Al-Sheikh Yazeed A.8Giesy John P.9Aboul-Soud Mourad A. M.10Department of Biotechnology, University of Science and Technology Bannu, Bannu, PakistanDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi ArabiaDepartment of Biochemistry, KMU Institute of Medical Sciences, Kohat26000, PakistanDepartment of Biotechnology, University of Science and Technology Bannu, Bannu, PakistanDepartment of Botany, University of Science and Technology Bannu, Bannu, PakistanDepartment of Biotechnology, University of Science and Technology Bannu, Bannu, PakistanDepartment of Biotechnology, University of Science and Technology Bannu, Bannu, PakistanDepartment of Chemistry, Kohat University of Science & Technology, Kohat26000, PakistanDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi ArabiaToxicology Centre, University of Saskatchewan, Saskatoon, SK S7N 5B3, CanadaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi ArabiaThe synthesis of silver nanoparticles (AgNPs) by the green method is favored as compared to chemical synthesis due to their appreciable properties of less toxicity and simple synthesis. The current study designed the biosynthesis of AgNPs in one step by using the plant Kickxia elatine (KE) extract and then investigated its inhibiting activity against rat’s brain acetylcholinesterase (AChE) ex vivo. Ultraviolet spectrum at 416 nm confirmed the formation of AgNPs. X-ray diffractometer calculated size was reported to be 42.47 nm. The SEM analysis confirmed spherical-shaped AgNPs. FT-IR suggested that the phytochemical groups present in the KE extract and their nanoparticles (NPs) are responsible for the biosynthesized of NPs. EDX analysis presented that Ag was the chief element with 61.67%. Both KE extract and AgNPs showed significant anti-AChE activity at 175 µg·mL−1. Statistical analysis showed that both KE and AgNPs exhibited non-competitive type inhibition against AChE, i.e. V max decreased (34.17–68.64% and 22.29–62.10%), while K m values remained constant. It is concluded that KE and AgNPs can be considered an inhibitor of rats’ brain AChE. Furthermore, the synthesis of AgNP-based drugs can be used as a cheaper and alternative option against diseases such as Alzheimer’s disease.https://doi.org/10.1515/gps-2023-0060kickxia elatineagnpsbrain homogenateacetylcholinesterasekinetics |
| spellingShingle | Huda Noor Ul Ghneim Hazem K. Fozia Fozia Ahmed Mushtaq Mushtaq Nadia Sher Naila Khan Rahmat Ali Ahmad Ijaz Al-Sheikh Yazeed A. Giesy John P. Aboul-Soud Mourad A. M. Green synthesis of Kickxia elatine-induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of Alzheimer’s disease Green Processing and Synthesis kickxia elatine agnps brain homogenate acetylcholinesterase kinetics |
| title | Green synthesis of Kickxia elatine-induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of Alzheimer’s disease |
| title_full | Green synthesis of Kickxia elatine-induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of Alzheimer’s disease |
| title_fullStr | Green synthesis of Kickxia elatine-induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of Alzheimer’s disease |
| title_full_unstemmed | Green synthesis of Kickxia elatine-induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of Alzheimer’s disease |
| title_short | Green synthesis of Kickxia elatine-induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of Alzheimer’s disease |
| title_sort | green synthesis of kickxia elatine induced silver nanoparticles and their role as anti acetylcholinesterase in the treatment of alzheimer s disease |
| topic | kickxia elatine agnps brain homogenate acetylcholinesterase kinetics |
| url | https://doi.org/10.1515/gps-2023-0060 |
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