Tn<i>6603</i>, a Carrier of Tn<i>5053</i> Family Transposons, Occurs in the Chromosome and in a Genomic Island of <i>Pseudomonas aeruginosa</i> Clinical Strains
Transposons of the <i>Pseudomonas</i><i>aeruginosa</i> accessory gene pool contribute to phenotype and to genome plasticity. We studied local <i>P. aeruginosa</i> strains to ascertain the encroachment of <i>mer</i>-type <i>res</i> site hunt...
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/1997 |
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| author | Vaheesan Rajabal Vilma A. Stanisich Steve Petrovski |
| author_facet | Vaheesan Rajabal Vilma A. Stanisich Steve Petrovski |
| author_sort | Vaheesan Rajabal |
| collection | DOAJ |
| description | Transposons of the <i>Pseudomonas</i><i>aeruginosa</i> accessory gene pool contribute to phenotype and to genome plasticity. We studied local <i>P. aeruginosa</i> strains to ascertain the encroachment of <i>mer</i>-type <i>res</i> site hunter transposons into clinical settings and their associations with other functional modules. Five different Tn<i>5053</i> family transposons were detected, all chromosomal. Some were solitary elements; one was in <i>res</i> of Tn<i>1013<sup>#</sup></i>, a relative of a reported carrier of <i>int</i>-type <i>res</i> site hunters (class 1 integrons), but most were in <i>res</i> of Tn<i>6603</i>, a new Tn<i>501</i>-related transposon of unknown phenotype. Most of the Tn<i>6603</i>::Tn elements, and some Tn<i>6603</i> and Tn<i>6603</i>::Tn elements found in GenBank sequences, were at identical sites in an hypothetical gene of <i>P. aeruginosa</i> genomic island PAGI-5v. The island in clonally differing strains was at either of two tRNA<sup>Lys</sup> loci, suggesting lateral transfer to these sites. This observation is consistent with the membership of the prototype PAGI-5 island to the ICE family of mobile genetic elements. Additionally, the <i>res</i> site hunters in the nested transposons occupied different positions in the Tn<i>6603</i> carrier. This suggested independent insertion events on five occasions at least. Tn<i>5053</i> family members that were <i>mer</i>-/<i>tni</i>-defective were found in Tn<i>6603</i>- and Tn<i>501</i>-like carriers in GenBank sequences of non-clinical <i>Pseudomonas</i> spp. The transposition events in these cases presumably utilized <i>tni</i> functions in trans, as can occur with class 1 integrons. We suggest that in the clinical context, <i>P. aeruginosa</i> strains that carry Tn<i>6603</i> alone or in PAGI-5v can serve to disseminate functional <i>res</i> site hunters; these in turn can provide the requisite <i>trans</i>-acting <i>tni</i> functions to assist in the dissemination of class 1 integrons, and hence of their associated antibiotic resistance determinants. |
| first_indexed | 2024-03-10T14:04:18Z |
| format | Article |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T14:04:18Z |
| publishDate | 2020-12-01 |
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| spelling | doaj.art-cd9b747cf4794468aad4aa96e609b5482023-11-21T00:51:22ZengMDPI AGMicroorganisms2076-26072020-12-01812199710.3390/microorganisms8121997Tn<i>6603</i>, a Carrier of Tn<i>5053</i> Family Transposons, Occurs in the Chromosome and in a Genomic Island of <i>Pseudomonas aeruginosa</i> Clinical StrainsVaheesan Rajabal0Vilma A. Stanisich1Steve Petrovski2Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC 3086, AustraliaDepartment of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC 3086, AustraliaDepartment of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC 3086, AustraliaTransposons of the <i>Pseudomonas</i><i>aeruginosa</i> accessory gene pool contribute to phenotype and to genome plasticity. We studied local <i>P. aeruginosa</i> strains to ascertain the encroachment of <i>mer</i>-type <i>res</i> site hunter transposons into clinical settings and their associations with other functional modules. Five different Tn<i>5053</i> family transposons were detected, all chromosomal. Some were solitary elements; one was in <i>res</i> of Tn<i>1013<sup>#</sup></i>, a relative of a reported carrier of <i>int</i>-type <i>res</i> site hunters (class 1 integrons), but most were in <i>res</i> of Tn<i>6603</i>, a new Tn<i>501</i>-related transposon of unknown phenotype. Most of the Tn<i>6603</i>::Tn elements, and some Tn<i>6603</i> and Tn<i>6603</i>::Tn elements found in GenBank sequences, were at identical sites in an hypothetical gene of <i>P. aeruginosa</i> genomic island PAGI-5v. The island in clonally differing strains was at either of two tRNA<sup>Lys</sup> loci, suggesting lateral transfer to these sites. This observation is consistent with the membership of the prototype PAGI-5 island to the ICE family of mobile genetic elements. Additionally, the <i>res</i> site hunters in the nested transposons occupied different positions in the Tn<i>6603</i> carrier. This suggested independent insertion events on five occasions at least. Tn<i>5053</i> family members that were <i>mer</i>-/<i>tni</i>-defective were found in Tn<i>6603</i>- and Tn<i>501</i>-like carriers in GenBank sequences of non-clinical <i>Pseudomonas</i> spp. The transposition events in these cases presumably utilized <i>tni</i> functions in trans, as can occur with class 1 integrons. We suggest that in the clinical context, <i>P. aeruginosa</i> strains that carry Tn<i>6603</i> alone or in PAGI-5v can serve to disseminate functional <i>res</i> site hunters; these in turn can provide the requisite <i>trans</i>-acting <i>tni</i> functions to assist in the dissemination of class 1 integrons, and hence of their associated antibiotic resistance determinants.https://www.mdpi.com/2076-2607/8/12/1997Tn<i>5053</i> family transposonsTn<i>6603</i>clinical <i>Pseudomonas aeruginosa</i>genomic island PAGI-5vclass 1 integronsnested transposons |
| spellingShingle | Vaheesan Rajabal Vilma A. Stanisich Steve Petrovski Tn<i>6603</i>, a Carrier of Tn<i>5053</i> Family Transposons, Occurs in the Chromosome and in a Genomic Island of <i>Pseudomonas aeruginosa</i> Clinical Strains Microorganisms Tn<i>5053</i> family transposons Tn<i>6603</i> clinical <i>Pseudomonas aeruginosa</i> genomic island PAGI-5v class 1 integrons nested transposons |
| title | Tn<i>6603</i>, a Carrier of Tn<i>5053</i> Family Transposons, Occurs in the Chromosome and in a Genomic Island of <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_full | Tn<i>6603</i>, a Carrier of Tn<i>5053</i> Family Transposons, Occurs in the Chromosome and in a Genomic Island of <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_fullStr | Tn<i>6603</i>, a Carrier of Tn<i>5053</i> Family Transposons, Occurs in the Chromosome and in a Genomic Island of <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_full_unstemmed | Tn<i>6603</i>, a Carrier of Tn<i>5053</i> Family Transposons, Occurs in the Chromosome and in a Genomic Island of <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_short | Tn<i>6603</i>, a Carrier of Tn<i>5053</i> Family Transposons, Occurs in the Chromosome and in a Genomic Island of <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_sort | tn i 6603 i a carrier of tn i 5053 i family transposons occurs in the chromosome and in a genomic island of i pseudomonas aeruginosa i clinical strains |
| topic | Tn<i>5053</i> family transposons Tn<i>6603</i> clinical <i>Pseudomonas aeruginosa</i> genomic island PAGI-5v class 1 integrons nested transposons |
| url | https://www.mdpi.com/2076-2607/8/12/1997 |
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