Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease
The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been util...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Analytica |
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| Online Access: | https://www.mdpi.com/2673-4532/4/3/21 |
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| author | Joshua Millar Luke Gibbons Catia Costa Ella Schneider Johanna von Gerichten Melanie J. Bailey Susan Campbell Catherine Duckett Sarah Doyle Laura M. Cole |
| author_facet | Joshua Millar Luke Gibbons Catia Costa Ella Schneider Johanna von Gerichten Melanie J. Bailey Susan Campbell Catherine Duckett Sarah Doyle Laura M. Cole |
| author_sort | Joshua Millar |
| collection | DOAJ |
| description | The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (<i>Rho<sup>−/−</sup></i>) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and <i>Rho<sup>−/−</sup></i> tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE). |
| first_indexed | 2024-03-10T23:07:54Z |
| format | Article |
| id | doaj.art-cd9d9cdaae364613b307ba81de363f2a |
| institution | Directory Open Access Journal |
| issn | 2673-4532 |
| language | English |
| last_indexed | 2024-03-10T23:07:54Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Analytica |
| spelling | doaj.art-cd9d9cdaae364613b307ba81de363f2a2023-11-19T09:13:30ZengMDPI AGAnalytica2673-45322023-07-014326427910.3390/analytica4030021Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular DiseaseJoshua Millar0Luke Gibbons1Catia Costa2Ella Schneider3Johanna von Gerichten4Melanie J. Bailey5Susan Campbell6Catherine Duckett7Sarah Doyle8Laura M. Cole9Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKImmunobiology Research Group, Department of Clinical Medicine, Trinity College Institute of Neuroscience (TCIN), School of Medicine, Trinity College Dublin (TCD), D02 R590 Dublin, IrelandIon Beam Centre, University of Surrey, Guildford GU2 7XH, UKIon Beam Centre, University of Surrey, Guildford GU2 7XH, UKDepartment of Chemistry, University of Surrey, Guildford GU2 7XH, UKDepartment of Chemistry, University of Surrey, Guildford GU2 7XH, UKCentre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKCentre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKImmunobiology Research Group, Department of Clinical Medicine, Trinity College Institute of Neuroscience (TCIN), School of Medicine, Trinity College Dublin (TCD), D02 R590 Dublin, IrelandCentre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKThe metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (<i>Rho<sup>−/−</sup></i>) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and <i>Rho<sup>−/−</sup></i> tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE).https://www.mdpi.com/2673-4532/4/3/21multimodallaser ablationICP-MSPIXEIBAocular tissue |
| spellingShingle | Joshua Millar Luke Gibbons Catia Costa Ella Schneider Johanna von Gerichten Melanie J. Bailey Susan Campbell Catherine Duckett Sarah Doyle Laura M. Cole Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease Analytica multimodal laser ablation ICP-MS PIXE IBA ocular tissue |
| title | Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease |
| title_full | Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease |
| title_fullStr | Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease |
| title_full_unstemmed | Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease |
| title_short | Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease |
| title_sort | multimodal imaging of metals in a retinal degeneration model to inform on ocular disease |
| topic | multimodal laser ablation ICP-MS PIXE IBA ocular tissue |
| url | https://www.mdpi.com/2673-4532/4/3/21 |
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