Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.
Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are sec...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2808357?pdf=render |
| _version_ | 1819020898101886976 |
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| author | Roy R Chaudhuri Mohammed Sebaihia Jon L Hobman Mark A Webber Denisse L Leyton Martin D Goldberg Adam F Cunningham Anthony Scott-Tucker Paul R Ferguson Christopher M Thomas Gad Frankel Christoph M Tang Edward G Dudley Ian S Roberts David A Rasko Mark J Pallen Julian Parkhill James P Nataro Nicholas R Thomson Ian R Henderson |
| author_facet | Roy R Chaudhuri Mohammed Sebaihia Jon L Hobman Mark A Webber Denisse L Leyton Martin D Goldberg Adam F Cunningham Anthony Scott-Tucker Paul R Ferguson Christopher M Thomas Gad Frankel Christoph M Tang Edward G Dudley Ian S Roberts David A Rasko Mark J Pallen Julian Parkhill James P Nataro Nicholas R Thomson Ian R Henderson |
| author_sort | Roy R Chaudhuri |
| collection | DOAJ |
| description | Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation.In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12.This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies. |
| first_indexed | 2024-12-21T03:58:31Z |
| format | Article |
| id | doaj.art-cd9e2f44dec2419cbd0ada1a80ba75e1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T03:58:31Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cd9e2f44dec2419cbd0ada1a80ba75e12022-12-21T19:16:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0151e880110.1371/journal.pone.0008801Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.Roy R ChaudhuriMohammed SebaihiaJon L HobmanMark A WebberDenisse L LeytonMartin D GoldbergAdam F CunninghamAnthony Scott-TuckerPaul R FergusonChristopher M ThomasGad FrankelChristoph M TangEdward G DudleyIan S RobertsDavid A RaskoMark J PallenJulian ParkhillJames P NataroNicholas R ThomsonIan R HendersonEscherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation.In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12.This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies.http://europepmc.org/articles/PMC2808357?pdf=render |
| spellingShingle | Roy R Chaudhuri Mohammed Sebaihia Jon L Hobman Mark A Webber Denisse L Leyton Martin D Goldberg Adam F Cunningham Anthony Scott-Tucker Paul R Ferguson Christopher M Thomas Gad Frankel Christoph M Tang Edward G Dudley Ian S Roberts David A Rasko Mark J Pallen Julian Parkhill James P Nataro Nicholas R Thomson Ian R Henderson Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. PLoS ONE |
| title | Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. |
| title_full | Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. |
| title_fullStr | Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. |
| title_full_unstemmed | Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. |
| title_short | Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. |
| title_sort | complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative escherichia coli strain 042 |
| url | http://europepmc.org/articles/PMC2808357?pdf=render |
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