Antiviral Activity of CD437 Against Mumps Virus
Many efforts have been dedicated to the discovery of antiviral drug candidates against the mumps virus (MuV); however, no specific drug has yet been approved. The development of efficient screening methods is a key factor for the discovery of antiviral candidates. In this study, we evaluated a scree...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.751909/full |
| _version_ | 1831677940521238528 |
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| author | Fumihiro Kato Yuichiro Nakatsu Keiko Murano Aika Wakata Toru Kubota Takayuki Hishiki Toshiyuki Yamaji Minoru Kidokoro Minoru Kidokoro Hiroshi Katoh Makoto Takeda |
| author_facet | Fumihiro Kato Yuichiro Nakatsu Keiko Murano Aika Wakata Toru Kubota Takayuki Hishiki Toshiyuki Yamaji Minoru Kidokoro Minoru Kidokoro Hiroshi Katoh Makoto Takeda |
| author_sort | Fumihiro Kato |
| collection | DOAJ |
| description | Many efforts have been dedicated to the discovery of antiviral drug candidates against the mumps virus (MuV); however, no specific drug has yet been approved. The development of efficient screening methods is a key factor for the discovery of antiviral candidates. In this study, we evaluated a screening method using an Aequorea coerulescens green fluorescent protein-expressing MuV infectious molecular clone. The application of this system to screen for active compounds against MuV replication revealed that CD437, a retinoid acid receptor agonist, has anti-MuV activity. The point of antiviral action was a late step(s) in the MuV life cycle. The replication of other paramyxoviruses was also inhibited by CD437. The induction of retinoic acid-inducible gene (RIG)-I expression is a reported mechanism for the antiviral activity of retinoids, but our results indicated that CD437 did not stimulate RIG-I expression. Indeed, we observed antiviral activity despite the absence of RIG-I, suggesting that CD437 antiviral activity does not require RIG-I induction. |
| first_indexed | 2024-12-20T04:50:23Z |
| format | Article |
| id | doaj.art-cd9e9428311f4df4a3b2f70f310d8440 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T04:50:23Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cd9e9428311f4df4a3b2f70f310d84402022-12-21T19:52:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.751909751909Antiviral Activity of CD437 Against Mumps VirusFumihiro Kato0Yuichiro Nakatsu1Keiko Murano2Aika Wakata3Toru Kubota4Takayuki Hishiki5Toshiyuki Yamaji6Minoru Kidokoro7Minoru Kidokoro8Hiroshi Katoh9Makoto Takeda10Department of Virology III, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Microbiology, Kanagawa Prefectural Institute of Public Health, Chigasaki, JapanDepartment of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Quality Assurance, Radiological Safety, and Information Management, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, JapanMany efforts have been dedicated to the discovery of antiviral drug candidates against the mumps virus (MuV); however, no specific drug has yet been approved. The development of efficient screening methods is a key factor for the discovery of antiviral candidates. In this study, we evaluated a screening method using an Aequorea coerulescens green fluorescent protein-expressing MuV infectious molecular clone. The application of this system to screen for active compounds against MuV replication revealed that CD437, a retinoid acid receptor agonist, has anti-MuV activity. The point of antiviral action was a late step(s) in the MuV life cycle. The replication of other paramyxoviruses was also inhibited by CD437. The induction of retinoic acid-inducible gene (RIG)-I expression is a reported mechanism for the antiviral activity of retinoids, but our results indicated that CD437 did not stimulate RIG-I expression. Indeed, we observed antiviral activity despite the absence of RIG-I, suggesting that CD437 antiviral activity does not require RIG-I induction.https://www.frontiersin.org/articles/10.3389/fmicb.2021.751909/fullCD437mumps virusknockout cellscreeningantiviral compound |
| spellingShingle | Fumihiro Kato Yuichiro Nakatsu Keiko Murano Aika Wakata Toru Kubota Takayuki Hishiki Toshiyuki Yamaji Minoru Kidokoro Minoru Kidokoro Hiroshi Katoh Makoto Takeda Antiviral Activity of CD437 Against Mumps Virus Frontiers in Microbiology CD437 mumps virus knockout cell screening antiviral compound |
| title | Antiviral Activity of CD437 Against Mumps Virus |
| title_full | Antiviral Activity of CD437 Against Mumps Virus |
| title_fullStr | Antiviral Activity of CD437 Against Mumps Virus |
| title_full_unstemmed | Antiviral Activity of CD437 Against Mumps Virus |
| title_short | Antiviral Activity of CD437 Against Mumps Virus |
| title_sort | antiviral activity of cd437 against mumps virus |
| topic | CD437 mumps virus knockout cell screening antiviral compound |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.751909/full |
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