Tractography of Porcine Meniscus Microstructure Using High-Resolution Diffusion Magnetic Resonance Imaging
To noninvasively evaluate the three-dimensional collagen fiber architecture of porcine meniscus using diffusion MRI, meniscal specimens were scanned using a 3D diffusion-weighted spin-echo pulse sequence at 7.0 T. The collagen fiber alignment was revealed in each voxel and the complex 3D collagen ne...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.876784/full |
| _version_ | 1818201461834645504 |
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| author | Jikai Shen Jikai Shen Qi Zhao Yi Qi Gary Cofer G. Allan Johnson G. Allan Johnson Nian Wang Nian Wang Nian Wang |
| author_facet | Jikai Shen Jikai Shen Qi Zhao Yi Qi Gary Cofer G. Allan Johnson G. Allan Johnson Nian Wang Nian Wang Nian Wang |
| author_sort | Jikai Shen |
| collection | DOAJ |
| description | To noninvasively evaluate the three-dimensional collagen fiber architecture of porcine meniscus using diffusion MRI, meniscal specimens were scanned using a 3D diffusion-weighted spin-echo pulse sequence at 7.0 T. The collagen fiber alignment was revealed in each voxel and the complex 3D collagen network was visualized for the entire meniscus using tractography. The proposed automatic segmentation methods divided the whole meniscus to different zones (Red-Red, Red-White, and White-White) and different parts (anterior, body, and posterior). The diffusion tensor imaging (DTI) metrics were quantified based on the segmentation results. The heatmap was generated to investigate the connections among different regions of meniscus. Strong zonal-dependent diffusion properties were demonstrated by DTI metrics. The fractional anisotropy (FA) value increased from 0.13 (White-White zone) to 0.26 (Red-Red zone) and the radial diffusivity (RD) value changed from 1.0 × 10-3 mm2/s (White-White zone) to 0.7 × 10-3 mm2/s (Red-Red zone). Coexistence of both radial and circumferential collagen fibers in the meniscus was evident by diffusion tractography. Weak connections were found between White-White zone and Red-Red zone in each part of the meniscus. The anterior part and posterior part were less connected, while the body part showed high connections to both anterior part and posterior part. The tractography based on diffusion MRI may provide a complementary method to study the integrity of meniscus and nondestructively visualize the 3D collagen fiber architecture. |
| first_indexed | 2024-12-12T02:53:56Z |
| format | Article |
| id | doaj.art-cd9f7fbacc49475d98e547a7af20f4d4 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-12T02:53:56Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-cd9f7fbacc49475d98e547a7af20f4d42022-12-22T00:40:49ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-05-011310.3389/fendo.2022.876784876784Tractography of Porcine Meniscus Microstructure Using High-Resolution Diffusion Magnetic Resonance ImagingJikai Shen0Jikai Shen1Qi Zhao2Yi Qi3Gary Cofer4G. Allan Johnson5G. Allan Johnson6Nian Wang7Nian Wang8Nian Wang9Department of Biomedical Engineering, Duke University, Durham, NC, United StatesSchool of Life Sciences, Westlake University, Hangzhou, ChinaPhysical Education Institute, Jimei University, Xiamen, ChinaDepartment of Radiology, Duke University School of Medicine, Durham, NC, United StatesDepartment of Radiology, Duke University School of Medicine, Durham, NC, United StatesDepartment of Biomedical Engineering, Duke University, Durham, NC, United StatesDepartment of Radiology, Duke University School of Medicine, Durham, NC, United StatesDepartment of Radiology, Duke University School of Medicine, Durham, NC, United StatesDepartment of Radiology and Imaging Sciences, Indiana University School of Medicine, Indianapolis, IN, United StatesStark Neurosciences Research Institute, Indiana University, Indianapolis, IN, United StatesTo noninvasively evaluate the three-dimensional collagen fiber architecture of porcine meniscus using diffusion MRI, meniscal specimens were scanned using a 3D diffusion-weighted spin-echo pulse sequence at 7.0 T. The collagen fiber alignment was revealed in each voxel and the complex 3D collagen network was visualized for the entire meniscus using tractography. The proposed automatic segmentation methods divided the whole meniscus to different zones (Red-Red, Red-White, and White-White) and different parts (anterior, body, and posterior). The diffusion tensor imaging (DTI) metrics were quantified based on the segmentation results. The heatmap was generated to investigate the connections among different regions of meniscus. Strong zonal-dependent diffusion properties were demonstrated by DTI metrics. The fractional anisotropy (FA) value increased from 0.13 (White-White zone) to 0.26 (Red-Red zone) and the radial diffusivity (RD) value changed from 1.0 × 10-3 mm2/s (White-White zone) to 0.7 × 10-3 mm2/s (Red-Red zone). Coexistence of both radial and circumferential collagen fibers in the meniscus was evident by diffusion tractography. Weak connections were found between White-White zone and Red-Red zone in each part of the meniscus. The anterior part and posterior part were less connected, while the body part showed high connections to both anterior part and posterior part. The tractography based on diffusion MRI may provide a complementary method to study the integrity of meniscus and nondestructively visualize the 3D collagen fiber architecture.https://www.frontiersin.org/articles/10.3389/fendo.2022.876784/fullDTIkneetractographymeniscusconnectivitysegmentation |
| spellingShingle | Jikai Shen Jikai Shen Qi Zhao Yi Qi Gary Cofer G. Allan Johnson G. Allan Johnson Nian Wang Nian Wang Nian Wang Tractography of Porcine Meniscus Microstructure Using High-Resolution Diffusion Magnetic Resonance Imaging Frontiers in Endocrinology DTI knee tractography meniscus connectivity segmentation |
| title | Tractography of Porcine Meniscus Microstructure Using High-Resolution Diffusion Magnetic Resonance Imaging |
| title_full | Tractography of Porcine Meniscus Microstructure Using High-Resolution Diffusion Magnetic Resonance Imaging |
| title_fullStr | Tractography of Porcine Meniscus Microstructure Using High-Resolution Diffusion Magnetic Resonance Imaging |
| title_full_unstemmed | Tractography of Porcine Meniscus Microstructure Using High-Resolution Diffusion Magnetic Resonance Imaging |
| title_short | Tractography of Porcine Meniscus Microstructure Using High-Resolution Diffusion Magnetic Resonance Imaging |
| title_sort | tractography of porcine meniscus microstructure using high resolution diffusion magnetic resonance imaging |
| topic | DTI knee tractography meniscus connectivity segmentation |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.876784/full |
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