Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse Skin
Tissue regeneration capabilities vary significantly throughout an organism’s lifespan. For example, mammals can fully regenerate until they reach specific developmental stages, after which they can only repair the tissue without restoring its original architecture and function. The high regenerative...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/9/1215 |
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| author | Valentina Castillo Pamela Díaz-Astudillo Rocío Corrales-Orovio Sebastián San Martín José Tomás Egaña |
| author_facet | Valentina Castillo Pamela Díaz-Astudillo Rocío Corrales-Orovio Sebastián San Martín José Tomás Egaña |
| author_sort | Valentina Castillo |
| collection | DOAJ |
| description | Tissue regeneration capabilities vary significantly throughout an organism’s lifespan. For example, mammals can fully regenerate until they reach specific developmental stages, after which they can only repair the tissue without restoring its original architecture and function. The high regenerative potential of fetal stages has been attributed to various factors, such as stem cells, the immune system, specific growth factors, and the presence of extracellular matrix molecules upon damage. To better understand the local differences between regenerative and reparative tissues, we conducted a comparative analysis of skin derived from mice at regenerative and reparative stages. Our findings show that both types of skin differ in their molecular composition, structure, and functionality. We observed a significant increase in cellular density, nucleic acid content, neutral lipid density, Collagen III, and glycosaminoglycans in regenerative skin compared with reparative skin. Additionally, regenerative skin had significantly higher porosity, metabolic activity, water absorption capacity, and elasticity than reparative skin. Finally, our results also revealed significant differences in lipid distribution, extracellular matrix pore size, and proteoglycans between the two groups. This study provides comprehensive data on the molecular and structural clues that enable full tissue regeneration in fetal stages, which could aid in developing new biomaterials and strategies for tissue engineering and regeneration. |
| first_indexed | 2024-03-11T04:22:36Z |
| format | Article |
| id | doaj.art-cda28329738746e595357b7ec0a233c0 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T04:22:36Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-cda28329738746e595357b7ec0a233c02023-11-17T22:42:54ZengMDPI AGCells2073-44092023-04-01129121510.3390/cells12091215Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse SkinValentina Castillo0Pamela Díaz-Astudillo1Rocío Corrales-Orovio2Sebastián San Martín3José Tomás Egaña4Institute for Biological and Medical Engineering, Schools of Engineering, Biological Sciences, and Medicine, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileBiomedical Research Center, School of Medicine, Universidad de Valparaiso, Valparaiso 2540064, ChileInstitute for Biological and Medical Engineering, Schools of Engineering, Biological Sciences, and Medicine, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileBiomedical Research Center, School of Medicine, Universidad de Valparaiso, Valparaiso 2540064, ChileInstitute for Biological and Medical Engineering, Schools of Engineering, Biological Sciences, and Medicine, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileTissue regeneration capabilities vary significantly throughout an organism’s lifespan. For example, mammals can fully regenerate until they reach specific developmental stages, after which they can only repair the tissue without restoring its original architecture and function. The high regenerative potential of fetal stages has been attributed to various factors, such as stem cells, the immune system, specific growth factors, and the presence of extracellular matrix molecules upon damage. To better understand the local differences between regenerative and reparative tissues, we conducted a comparative analysis of skin derived from mice at regenerative and reparative stages. Our findings show that both types of skin differ in their molecular composition, structure, and functionality. We observed a significant increase in cellular density, nucleic acid content, neutral lipid density, Collagen III, and glycosaminoglycans in regenerative skin compared with reparative skin. Additionally, regenerative skin had significantly higher porosity, metabolic activity, water absorption capacity, and elasticity than reparative skin. Finally, our results also revealed significant differences in lipid distribution, extracellular matrix pore size, and proteoglycans between the two groups. This study provides comprehensive data on the molecular and structural clues that enable full tissue regeneration in fetal stages, which could aid in developing new biomaterials and strategies for tissue engineering and regeneration.https://www.mdpi.com/2073-4409/12/9/1215tissue regenerationtissue repairskinextracellular matrix |
| spellingShingle | Valentina Castillo Pamela Díaz-Astudillo Rocío Corrales-Orovio Sebastián San Martín José Tomás Egaña Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse Skin Cells tissue regeneration tissue repair skin extracellular matrix |
| title | Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse Skin |
| title_full | Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse Skin |
| title_fullStr | Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse Skin |
| title_full_unstemmed | Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse Skin |
| title_short | Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse Skin |
| title_sort | comprehensive characterization of tissues derived from animals at different regenerative stages a comparative analysis between fetal and adult mouse skin |
| topic | tissue regeneration tissue repair skin extracellular matrix |
| url | https://www.mdpi.com/2073-4409/12/9/1215 |
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