Protective Effect of <i>Urtica dioica</i> Extract against Oxidative Stress in Human Skin Fibroblasts
<i>Urtica dioica</i> is a species with well-established significance in folk medicine in many countries. It was utilized to support the treatment of arthritis, allergies, and urinary tract disorders; however, the substantial presence of antioxidants suggests that nettle extract could als...
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MDPI AG
2023-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/11/2182 |
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| author | Agnieszka Skalska-Kamińska Weronika Wójciak Magdalena Żuk Roman Paduch Magdalena Wójciak |
| author_facet | Agnieszka Skalska-Kamińska Weronika Wójciak Magdalena Żuk Roman Paduch Magdalena Wójciak |
| author_sort | Agnieszka Skalska-Kamińska |
| collection | DOAJ |
| description | <i>Urtica dioica</i> is a species with well-established significance in folk medicine in many countries. It was utilized to support the treatment of arthritis, allergies, and urinary tract disorders; however, the substantial presence of antioxidants suggests that nettle extract could also have a positive impact on the skin. The objective of this study was to assess the impact of nettle extract on human skin fibroblasts subjected to oxidative stress. Various solvents were tested to prepare an extract rich in polyphenolic compounds with high antioxidant potential. The chemical composition was determined using ultra-high-performance liquid chromatography with mass spectrometry (UPLC-DAD-MS). H<sub>2</sub>O<sub>2</sub> treatment was used to induce oxidative stress and cell viability, and the metabolism was evaluated through NR and MTT assays. Our study demonstrated that extraction with 80% ethanol, followed by the drying and re-dissolving of the extract in pure water, was more efficient than direct extraction with water. This yielded an extract rich in polyphenolic compounds, with chlorogenic acid and caffeoylmalic acid as the predominant compounds, averaging 64.9 and 114.4 µg/mL, respectively. The extract exhibited antioxidant properties in the DPPH and ABTS assays. Furthermore, it did not exhibit cytotoxicity and did not negatively affect cell metabolism. In addition, it effectively reduced ROS in the H<sub>2</sub>O<sub>2</sub>-stimulated cells, and at the highest concentration tested, the ROS levels returned to those of the untreated control. The extract also protected against H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity. The cell viability was maintained at the level of the untreated control when the cells were pretreated with the extract before H<sub>2</sub>O<sub>2</sub> exposure. These findings indicate that <i>U. dioica</i> extract is a valuable and safe additive in skincare products. |
| first_indexed | 2024-03-09T16:40:30Z |
| format | Article |
| id | doaj.art-cda5cc8b660944839e7de26b009017d7 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T16:40:30Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-cda5cc8b660944839e7de26b009017d72023-11-24T14:52:29ZengMDPI AGLife2075-17292023-11-011311218210.3390/life13112182Protective Effect of <i>Urtica dioica</i> Extract against Oxidative Stress in Human Skin FibroblastsAgnieszka Skalska-Kamińska0Weronika Wójciak1Magdalena Żuk2Roman Paduch3Magdalena Wójciak4Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Virology and Immunology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, 19 Akademicka Street, 20-033 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland<i>Urtica dioica</i> is a species with well-established significance in folk medicine in many countries. It was utilized to support the treatment of arthritis, allergies, and urinary tract disorders; however, the substantial presence of antioxidants suggests that nettle extract could also have a positive impact on the skin. The objective of this study was to assess the impact of nettle extract on human skin fibroblasts subjected to oxidative stress. Various solvents were tested to prepare an extract rich in polyphenolic compounds with high antioxidant potential. The chemical composition was determined using ultra-high-performance liquid chromatography with mass spectrometry (UPLC-DAD-MS). H<sub>2</sub>O<sub>2</sub> treatment was used to induce oxidative stress and cell viability, and the metabolism was evaluated through NR and MTT assays. Our study demonstrated that extraction with 80% ethanol, followed by the drying and re-dissolving of the extract in pure water, was more efficient than direct extraction with water. This yielded an extract rich in polyphenolic compounds, with chlorogenic acid and caffeoylmalic acid as the predominant compounds, averaging 64.9 and 114.4 µg/mL, respectively. The extract exhibited antioxidant properties in the DPPH and ABTS assays. Furthermore, it did not exhibit cytotoxicity and did not negatively affect cell metabolism. In addition, it effectively reduced ROS in the H<sub>2</sub>O<sub>2</sub>-stimulated cells, and at the highest concentration tested, the ROS levels returned to those of the untreated control. The extract also protected against H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity. The cell viability was maintained at the level of the untreated control when the cells were pretreated with the extract before H<sub>2</sub>O<sub>2</sub> exposure. These findings indicate that <i>U. dioica</i> extract is a valuable and safe additive in skincare products.https://www.mdpi.com/2075-1729/13/11/2182nettlefibroblastsantioxidantschromatographic analysisoxidative stress |
| spellingShingle | Agnieszka Skalska-Kamińska Weronika Wójciak Magdalena Żuk Roman Paduch Magdalena Wójciak Protective Effect of <i>Urtica dioica</i> Extract against Oxidative Stress in Human Skin Fibroblasts Life nettle fibroblasts antioxidants chromatographic analysis oxidative stress |
| title | Protective Effect of <i>Urtica dioica</i> Extract against Oxidative Stress in Human Skin Fibroblasts |
| title_full | Protective Effect of <i>Urtica dioica</i> Extract against Oxidative Stress in Human Skin Fibroblasts |
| title_fullStr | Protective Effect of <i>Urtica dioica</i> Extract against Oxidative Stress in Human Skin Fibroblasts |
| title_full_unstemmed | Protective Effect of <i>Urtica dioica</i> Extract against Oxidative Stress in Human Skin Fibroblasts |
| title_short | Protective Effect of <i>Urtica dioica</i> Extract against Oxidative Stress in Human Skin Fibroblasts |
| title_sort | protective effect of i urtica dioica i extract against oxidative stress in human skin fibroblasts |
| topic | nettle fibroblasts antioxidants chromatographic analysis oxidative stress |
| url | https://www.mdpi.com/2075-1729/13/11/2182 |
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