Rapid and Quantitative Determination of Sildenafil in Cocktail Based on Surface Enhanced Raman Spectroscopy

Sildenafil (SD) and its related compounds are the most common adulterants found in herbal preparations used as sexual enhancer or man’s virility products. However, the abuse of SD threatens human health such as through headache, back pain, rhinitis, etc. Therefore, it is important to accur...

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Main Authors: Lei Lin, Fangfang Qu, Pengcheng Nie, Hui Zhang, Bingquan Chu, Yong He
Format: Article
Language:English
Published: MDPI AG 2019-05-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/24/9/1790
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author Lei Lin
Fangfang Qu
Pengcheng Nie
Hui Zhang
Bingquan Chu
Yong He
author_facet Lei Lin
Fangfang Qu
Pengcheng Nie
Hui Zhang
Bingquan Chu
Yong He
author_sort Lei Lin
collection DOAJ
description Sildenafil (SD) and its related compounds are the most common adulterants found in herbal preparations used as sexual enhancer or man&#8217;s virility products. However, the abuse of SD threatens human health such as through headache, back pain, rhinitis, etc. Therefore, it is important to accurately detect the presence of SD in alcoholic beverages. In this study, the Opto Trace Raman 202 (OTR 202) was used as a surface-enhanced Raman spectroscopy (SERS) active colloids to detect SD. The results demonstrated that the limit of detection (LOD) of SD was found to be as low as 0.1 mg/L. Moreover, 1235, 1401, 1530, and 1584 cm<sup>&#8722;1</sup> could be qualitatively determined as SD characteristic peaks. In a practical application, SD in cocktail could be easily detected using SERS based on OTR 202. Also, there was a good linear correlation between the intensity of Raman peaks at 1235, 1401, 1530, and 1584 cm<sup>&#8722;1</sup> and the logarithm of SD concentration in cocktail was in the range of 0.1&#8722;10 mg/L (0.9822 &lt; R<sup>2</sup> &lt; 0.9860). The relative standard deviation (RSD) was less than 12.7% and the recovery ranged from 93.0%&#8722;105.8%. Moreover, the original 500&#8722;1700 cm<sup>&#8722;1</sup> SERS spectra were pretreated and the partial least squares (PLS) was applied to establish the prediction model between SERS spectra and SD content in cocktail and the highest determination coefficient (Rp<sup>2</sup>) reached 0.9856. In summary, the SD in cocktail could be rapidly and quantitatively determined by SERS, which was beneficial to provide a rapid and accurate scheme for the detection of SD in alcoholic beverages.
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spelling doaj.art-cda62c86dadf4529add01ca797ea5b392022-12-22T00:30:23ZengMDPI AGMolecules1420-30492019-05-01249179010.3390/molecules24091790molecules24091790Rapid and Quantitative Determination of Sildenafil in Cocktail Based on Surface Enhanced Raman SpectroscopyLei Lin0Fangfang Qu1Pengcheng Nie2Hui Zhang3Bingquan Chu4Yong He5College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaSildenafil (SD) and its related compounds are the most common adulterants found in herbal preparations used as sexual enhancer or man&#8217;s virility products. However, the abuse of SD threatens human health such as through headache, back pain, rhinitis, etc. Therefore, it is important to accurately detect the presence of SD in alcoholic beverages. In this study, the Opto Trace Raman 202 (OTR 202) was used as a surface-enhanced Raman spectroscopy (SERS) active colloids to detect SD. The results demonstrated that the limit of detection (LOD) of SD was found to be as low as 0.1 mg/L. Moreover, 1235, 1401, 1530, and 1584 cm<sup>&#8722;1</sup> could be qualitatively determined as SD characteristic peaks. In a practical application, SD in cocktail could be easily detected using SERS based on OTR 202. Also, there was a good linear correlation between the intensity of Raman peaks at 1235, 1401, 1530, and 1584 cm<sup>&#8722;1</sup> and the logarithm of SD concentration in cocktail was in the range of 0.1&#8722;10 mg/L (0.9822 &lt; R<sup>2</sup> &lt; 0.9860). The relative standard deviation (RSD) was less than 12.7% and the recovery ranged from 93.0%&#8722;105.8%. Moreover, the original 500&#8722;1700 cm<sup>&#8722;1</sup> SERS spectra were pretreated and the partial least squares (PLS) was applied to establish the prediction model between SERS spectra and SD content in cocktail and the highest determination coefficient (Rp<sup>2</sup>) reached 0.9856. In summary, the SD in cocktail could be rapidly and quantitatively determined by SERS, which was beneficial to provide a rapid and accurate scheme for the detection of SD in alcoholic beverages.https://www.mdpi.com/1420-3049/24/9/1790sildenafilcocktailsurface enhanced Raman spectroscopyquantitative determinationpartial least squares
spellingShingle Lei Lin
Fangfang Qu
Pengcheng Nie
Hui Zhang
Bingquan Chu
Yong He
Rapid and Quantitative Determination of Sildenafil in Cocktail Based on Surface Enhanced Raman Spectroscopy
Molecules
sildenafil
cocktail
surface enhanced Raman spectroscopy
quantitative determination
partial least squares
title Rapid and Quantitative Determination of Sildenafil in Cocktail Based on Surface Enhanced Raman Spectroscopy
title_full Rapid and Quantitative Determination of Sildenafil in Cocktail Based on Surface Enhanced Raman Spectroscopy
title_fullStr Rapid and Quantitative Determination of Sildenafil in Cocktail Based on Surface Enhanced Raman Spectroscopy
title_full_unstemmed Rapid and Quantitative Determination of Sildenafil in Cocktail Based on Surface Enhanced Raman Spectroscopy
title_short Rapid and Quantitative Determination of Sildenafil in Cocktail Based on Surface Enhanced Raman Spectroscopy
title_sort rapid and quantitative determination of sildenafil in cocktail based on surface enhanced raman spectroscopy
topic sildenafil
cocktail
surface enhanced Raman spectroscopy
quantitative determination
partial least squares
url https://www.mdpi.com/1420-3049/24/9/1790
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AT fangfangqu rapidandquantitativedeterminationofsildenafilincocktailbasedonsurfaceenhancedramanspectroscopy
AT pengchengnie rapidandquantitativedeterminationofsildenafilincocktailbasedonsurfaceenhancedramanspectroscopy
AT huizhang rapidandquantitativedeterminationofsildenafilincocktailbasedonsurfaceenhancedramanspectroscopy
AT bingquanchu rapidandquantitativedeterminationofsildenafilincocktailbasedonsurfaceenhancedramanspectroscopy
AT yonghe rapidandquantitativedeterminationofsildenafilincocktailbasedonsurfaceenhancedramanspectroscopy