A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity
<p>Abstract</p> <p>Background</p> <p>The spread of drug resistance in malaria parasites and the limited number of effective drugs for treatment indicates the need for new anti-malarial compounds. Current assays evaluating drugs against <it>Plasmodium falciparum<...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-08-01
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| Series: | Malaria Journal |
| Subjects: | |
| Online Access: | http://www.malariajournal.com/content/11/1/272 |
| _version_ | 1828454093977288704 |
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| author | Men Tran Huy Nguyen Trang Dai Thi Shuaibu Mohammed Hirayama Kenji Kamei Kaeko |
| author_facet | Men Tran Huy Nguyen Trang Dai Thi Shuaibu Mohammed Hirayama Kenji Kamei Kaeko |
| author_sort | Men Tran |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The spread of drug resistance in malaria parasites and the limited number of effective drugs for treatment indicates the need for new anti-malarial compounds. Current assays evaluating drugs against <it>Plasmodium falciparum</it> require expensive materials and equipment, thus limiting the search for new drugs, particularly in developing countries. This study describes an inexpensive procedure that is based on the advantage of a positive correlation between the haemozoin level of infected erythrocytes and parasite load.</p> <p>Methods</p> <p>The relationship between parasitaemia and the haemozoin level of infected erythrocytes was investigated after converting haemozoin into monomeric haem. The 50% inhibitory concentration (IC<sub>50</sub>) values of chloroquine, quinine, artemisinin, quinidine and clotrimazole against <it>P. falciparum</it> K1 and 9A strains were determined using the novel assay method.</p> <p>Results</p> <p>The haemozoin of parasites was extracted and converted into monomeric haem, allowing the use of a colorimeter to efficiently and rapidly measure the growth of the parasites. There was a strong and direct linear relationship between the absorbance of haem converted from haemozoin and the percentage of the parasite (R<sup>2</sup> = 0.9929). Furthermore, the IC<sub>50</sub> values of drugs were within the range of the values previously reported.</p> <p>Conclusion</p> <p>The haemozoin-based colorimetric assay can be considered as an alternative, simple, robust, inexpensive and convenient method, making it applicable in developing countries.</p> |
| first_indexed | 2024-12-11T00:17:18Z |
| format | Article |
| id | doaj.art-cda90e0ff2784d94bed654f6e7f89b42 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-11T00:17:18Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-cda90e0ff2784d94bed654f6e7f89b422022-12-22T01:27:54ZengBMCMalaria Journal1475-28752012-08-0111127210.1186/1475-2875-11-272A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activityMen TranHuy NguyenTrang Dai ThiShuaibu MohammedHirayama KenjiKamei Kaeko<p>Abstract</p> <p>Background</p> <p>The spread of drug resistance in malaria parasites and the limited number of effective drugs for treatment indicates the need for new anti-malarial compounds. Current assays evaluating drugs against <it>Plasmodium falciparum</it> require expensive materials and equipment, thus limiting the search for new drugs, particularly in developing countries. This study describes an inexpensive procedure that is based on the advantage of a positive correlation between the haemozoin level of infected erythrocytes and parasite load.</p> <p>Methods</p> <p>The relationship between parasitaemia and the haemozoin level of infected erythrocytes was investigated after converting haemozoin into monomeric haem. The 50% inhibitory concentration (IC<sub>50</sub>) values of chloroquine, quinine, artemisinin, quinidine and clotrimazole against <it>P. falciparum</it> K1 and 9A strains were determined using the novel assay method.</p> <p>Results</p> <p>The haemozoin of parasites was extracted and converted into monomeric haem, allowing the use of a colorimeter to efficiently and rapidly measure the growth of the parasites. There was a strong and direct linear relationship between the absorbance of haem converted from haemozoin and the percentage of the parasite (R<sup>2</sup> = 0.9929). Furthermore, the IC<sub>50</sub> values of drugs were within the range of the values previously reported.</p> <p>Conclusion</p> <p>The haemozoin-based colorimetric assay can be considered as an alternative, simple, robust, inexpensive and convenient method, making it applicable in developing countries.</p>http://www.malariajournal.com/content/11/1/272Anti-malarialAssayHaemozoinMalaria |
| spellingShingle | Men Tran Huy Nguyen Trang Dai Thi Shuaibu Mohammed Hirayama Kenji Kamei Kaeko A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity Malaria Journal Anti-malarial Assay Haemozoin Malaria |
| title | A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity |
| title_full | A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity |
| title_fullStr | A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity |
| title_full_unstemmed | A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity |
| title_short | A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity |
| title_sort | simple and inexpensive haemozoin based colorimetric method to evaluate anti malarial drug activity |
| topic | Anti-malarial Assay Haemozoin Malaria |
| url | http://www.malariajournal.com/content/11/1/272 |
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