Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent eviden...
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/21/5216 |
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| author | Jung-Won Choi Soyeon Lim Jung Hwa Kang Sung Hwan Hwang Ki-Chul Hwang Sang Woo Kim Seahyoung Lee |
| author_facet | Jung-Won Choi Soyeon Lim Jung Hwa Kang Sung Hwan Hwang Ki-Chul Hwang Sang Woo Kim Seahyoung Lee |
| author_sort | Jung-Won Choi |
| collection | DOAJ |
| description | Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent evidence indicates that even immune-cell-released vesicles such as NK-cell-derived exosomes also exert anticancer effect. Nevertheless, the underlying mechanisms remain elusive. In the present study, the anticancer potential of isolated extracellular vesicles (EVs) from expanded and activated NK-cell-enriched lymphocytes (NKLs) prepared by house-developed protocol was evaluated both in vitro and in vivo. Moreover, isolated EVs were characterized by using two-dimensional electrophoresis (2-DE)-based proteome and network analysis, and functional study using identified factors was performed. Our data indicated that the EVs from expanded and active NKLs had anticancer properties, and a number of molecules, such as Fas ligand, TRAIL, NKG2D, β-actin, and fibrinogen, were identified as effector candidates based on the proteome analysis and functional study. The results of the present study suggest the possibility of NK-cell-derived EVs as a viable immunotherapeutic strategy for cancer. |
| first_indexed | 2024-03-10T14:59:14Z |
| format | Article |
| id | doaj.art-cda944b4e12047688b8e236275c9e4e3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:59:14Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cda944b4e12047688b8e236275c9e4e32023-11-20T20:20:02ZengMDPI AGMolecules1420-30492020-11-012521521610.3390/molecules25215216Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer EffectorsJung-Won Choi0Soyeon Lim1Jung Hwa Kang2Sung Hwan Hwang3Ki-Chul Hwang4Sang Woo Kim5Seahyoung Lee6Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 210-701, KoreaInstitute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 210-701, KoreaIMMUNISBIO Co. Ltd., B2F MTP Mall, International St. Mary’s Hospital, Incheon Metropolitan City 22711, KoreaIMMUNISBIO Co. Ltd., B2F MTP Mall, International St. Mary’s Hospital, Incheon Metropolitan City 22711, KoreaInstitute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 210-701, KoreaInstitute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 210-701, KoreaInstitute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 210-701, KoreaCancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent evidence indicates that even immune-cell-released vesicles such as NK-cell-derived exosomes also exert anticancer effect. Nevertheless, the underlying mechanisms remain elusive. In the present study, the anticancer potential of isolated extracellular vesicles (EVs) from expanded and activated NK-cell-enriched lymphocytes (NKLs) prepared by house-developed protocol was evaluated both in vitro and in vivo. Moreover, isolated EVs were characterized by using two-dimensional electrophoresis (2-DE)-based proteome and network analysis, and functional study using identified factors was performed. Our data indicated that the EVs from expanded and active NKLs had anticancer properties, and a number of molecules, such as Fas ligand, TRAIL, NKG2D, β-actin, and fibrinogen, were identified as effector candidates based on the proteome analysis and functional study. The results of the present study suggest the possibility of NK-cell-derived EVs as a viable immunotherapeutic strategy for cancer.https://www.mdpi.com/1420-3049/25/21/5216extracellular vesiclesnatural-killer-enriched lymphocytes (NKL)anticancer immunotherapyproteome analysis |
| spellingShingle | Jung-Won Choi Soyeon Lim Jung Hwa Kang Sung Hwan Hwang Ki-Chul Hwang Sang Woo Kim Seahyoung Lee Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors Molecules extracellular vesicles natural-killer-enriched lymphocytes (NKL) anticancer immunotherapy proteome analysis |
| title | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
| title_full | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
| title_fullStr | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
| title_full_unstemmed | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
| title_short | Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors |
| title_sort | proteome analysis of human natural killer cell derived extracellular vesicles for identification of anticancer effectors |
| topic | extracellular vesicles natural-killer-enriched lymphocytes (NKL) anticancer immunotherapy proteome analysis |
| url | https://www.mdpi.com/1420-3049/25/21/5216 |
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