| Summary: | Reactive oxygen species (ROS) regulate several aspects of cell physiology in filamentous fungi including the antioxidant response and development. However, little is known about the signaling pathways involved in these processes. Here, we report <i>Aspergillus nidulans</i> global phosphoproteome during mycelial growth and show that under these conditions, H<sub>2</sub>O<sub>2</sub> induces major changes in protein phosphorylation. Among the 1964 phosphoproteins we identified, H<sub>2</sub>O<sub>2</sub> induced the phosphorylation of 131 proteins at one or more sites as well as the dephosphorylation of a larger set of proteins. A detailed analysis of these phosphoproteins shows that H<sub>2</sub>O<sub>2</sub> affected the phosphorylation of critical regulatory nodes of phosphoinositide, MAPK, and TOR signaling as well as the phosphorylation of multiple proteins involved in the regulation of gene expression, primary and secondary metabolism, and development. Our results provide a novel and extensive protein phosphorylation landscape in <i>A. nidulans</i>, indicating that H<sub>2</sub>O<sub>2</sub> induces a shift in general metabolism from anabolic to catabolic, and the activation of multiple stress survival pathways. Our results expand the significance of H<sub>2</sub>O<sub>2</sub> in eukaryotic cell signaling.
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