H<sub>2</sub>O<sub>2</sub> Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in <i>Aspergillus nidulans</i>
Reactive oxygen species (ROS) regulate several aspects of cell physiology in filamentous fungi including the antioxidant response and development. However, little is known about the signaling pathways involved in these processes. Here, we report <i>Aspergillus nidulans</i> global phospho...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/8/624 |
| _version_ | 1797523295738789888 |
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| author | Ulises Carrasco-Navarro Jesús Aguirre |
| author_facet | Ulises Carrasco-Navarro Jesús Aguirre |
| author_sort | Ulises Carrasco-Navarro |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) regulate several aspects of cell physiology in filamentous fungi including the antioxidant response and development. However, little is known about the signaling pathways involved in these processes. Here, we report <i>Aspergillus nidulans</i> global phosphoproteome during mycelial growth and show that under these conditions, H<sub>2</sub>O<sub>2</sub> induces major changes in protein phosphorylation. Among the 1964 phosphoproteins we identified, H<sub>2</sub>O<sub>2</sub> induced the phosphorylation of 131 proteins at one or more sites as well as the dephosphorylation of a larger set of proteins. A detailed analysis of these phosphoproteins shows that H<sub>2</sub>O<sub>2</sub> affected the phosphorylation of critical regulatory nodes of phosphoinositide, MAPK, and TOR signaling as well as the phosphorylation of multiple proteins involved in the regulation of gene expression, primary and secondary metabolism, and development. Our results provide a novel and extensive protein phosphorylation landscape in <i>A. nidulans</i>, indicating that H<sub>2</sub>O<sub>2</sub> induces a shift in general metabolism from anabolic to catabolic, and the activation of multiple stress survival pathways. Our results expand the significance of H<sub>2</sub>O<sub>2</sub> in eukaryotic cell signaling. |
| first_indexed | 2024-03-10T08:41:22Z |
| format | Article |
| id | doaj.art-cdabe835286f4591a9910fdb3151a67c |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T08:41:22Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-cdabe835286f4591a9910fdb3151a67c2023-11-22T08:16:55ZengMDPI AGJournal of Fungi2309-608X2021-07-017862410.3390/jof7080624H<sub>2</sub>O<sub>2</sub> Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in <i>Aspergillus nidulans</i>Ulises Carrasco-Navarro0Jesús Aguirre1Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, Ciudad de México 04510, MexicoDepartamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, Ciudad de México 04510, MexicoReactive oxygen species (ROS) regulate several aspects of cell physiology in filamentous fungi including the antioxidant response and development. However, little is known about the signaling pathways involved in these processes. Here, we report <i>Aspergillus nidulans</i> global phosphoproteome during mycelial growth and show that under these conditions, H<sub>2</sub>O<sub>2</sub> induces major changes in protein phosphorylation. Among the 1964 phosphoproteins we identified, H<sub>2</sub>O<sub>2</sub> induced the phosphorylation of 131 proteins at one or more sites as well as the dephosphorylation of a larger set of proteins. A detailed analysis of these phosphoproteins shows that H<sub>2</sub>O<sub>2</sub> affected the phosphorylation of critical regulatory nodes of phosphoinositide, MAPK, and TOR signaling as well as the phosphorylation of multiple proteins involved in the regulation of gene expression, primary and secondary metabolism, and development. Our results provide a novel and extensive protein phosphorylation landscape in <i>A. nidulans</i>, indicating that H<sub>2</sub>O<sub>2</sub> induces a shift in general metabolism from anabolic to catabolic, and the activation of multiple stress survival pathways. Our results expand the significance of H<sub>2</sub>O<sub>2</sub> in eukaryotic cell signaling.https://www.mdpi.com/2309-608X/7/8/624ROS signalingstressphosphoinositide signalingMAPKTORnitrogen metabolism |
| spellingShingle | Ulises Carrasco-Navarro Jesús Aguirre H<sub>2</sub>O<sub>2</sub> Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in <i>Aspergillus nidulans</i> Journal of Fungi ROS signaling stress phosphoinositide signaling MAPK TOR nitrogen metabolism |
| title | H<sub>2</sub>O<sub>2</sub> Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in <i>Aspergillus nidulans</i> |
| title_full | H<sub>2</sub>O<sub>2</sub> Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in <i>Aspergillus nidulans</i> |
| title_fullStr | H<sub>2</sub>O<sub>2</sub> Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in <i>Aspergillus nidulans</i> |
| title_full_unstemmed | H<sub>2</sub>O<sub>2</sub> Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in <i>Aspergillus nidulans</i> |
| title_short | H<sub>2</sub>O<sub>2</sub> Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in <i>Aspergillus nidulans</i> |
| title_sort | h sub 2 sub o sub 2 sub induces major phosphorylation changes in critical regulators of signal transduction gene expression metabolism and developmental networks in i aspergillus nidulans i |
| topic | ROS signaling stress phosphoinositide signaling MAPK TOR nitrogen metabolism |
| url | https://www.mdpi.com/2309-608X/7/8/624 |
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