Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses

Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses

Viral metagenomics next-generation sequencing (mNGS) is increasingly being used to characterize the human virome. The impact of viral nucleic extraction on virome profiling has been poorly studied. Here, we aimed to compare the sensitivity and sample and reagent contamination of three extraction met...

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Bibliographic Details
Main Authors: Marina Sabatier, Antonin Bal, Grégory Destras, Hadrien Regue, Grégory Quéromès, Valérie Cheynet, Bruno Lina, Claire Bardel, Karen Brengel-Pesce, Vincent Navratil, Laurence Josset
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Microorganisms
Subjects:
viral metagenomics
next-generation sequencing
acid nucleic extraction
sample cross-contamination
kitome
Online Access:https://www.mdpi.com/2076-2607/8/10/1539

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