Molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis cases
This study aimed to investigate the presence and distribution of virulence determinants and their antimicrobial susceptibilities of 44 Pseudomonas aeruginosa isolates obtained from clinical bovine mastitis cases. In addition, selected 6 P. aeruginosa isolates were further characterized using whole-g...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2022-11-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2951 |
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| author | Özkan ASLANTAŞ Süheyla TÜRKYILMAZ Oktay KESKİN Ayfer GÜLLÜ YÜCETEPE Kaan BÜYÜKALTAY |
| author_facet | Özkan ASLANTAŞ Süheyla TÜRKYILMAZ Oktay KESKİN Ayfer GÜLLÜ YÜCETEPE Kaan BÜYÜKALTAY |
| author_sort | Özkan ASLANTAŞ |
| collection | DOAJ |
| description | This study aimed to investigate the presence and distribution of virulence determinants and their antimicrobial susceptibilities of
44 Pseudomonas aeruginosa isolates obtained from clinical bovine mastitis cases. In addition, selected 6 P. aeruginosa isolates were further
characterized using whole-genome sequencing (WGS). Based on the presence of T3SS-related genes, 25% of the isolates were found to carry
exoU and/or exoS genes belonging to invasive (exoU-/exoS+, 18.2%) and cytotoxic (exoU+/exoS-, 6.8%) strains. But, none of the isolates
carried both exoU and exoS genes. In terms of other virulence genes examined, various virulence gene profiles were observed among the
isolates. The majority of the isolates (72.7%) were susceptible to all tested antimicrobials. Resistance rates to ciprofloxacin and carbapenems
(imipenem and meropenem) were determined as 25% and 4.5%, respectively. WGS analysis indicated the presence of different resistome,
but fosA genes in all isolates, and different combinations of mutations in gyrA, parC, oprD, efflux pump, and genes playing a role in the
regulation of ampC gene expression. Different sequence types (STs) and serotypes were found in representative isolates with the occurrence
of the O11-ST235 clone, which is a worldwide multidrug-resistant high-risk clone representing a serious public health threat. The findings
of this study provide valuable information on P. aeruginosa isolated from clinical bovine mastitis cases and current antimicrobial resistance
levels and virulence determinants. |
| first_indexed | 2024-03-13T04:41:48Z |
| format | Article |
| id | doaj.art-cdb174b5b7bf4121af7f58013c9c46a2 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:41:48Z |
| publishDate | 2022-11-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-cdb174b5b7bf4121af7f58013c9c46a22023-06-19T05:49:02ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512022-11-0128674775910.9775/kvfd.2022.281182951Molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis casesÖzkan ASLANTAŞ0Süheyla TÜRKYILMAZ1Oktay KESKİN2Ayfer GÜLLÜ YÜCETEPE3Kaan BÜYÜKALTAY4Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Microbiology, TR-31060 Hatay - TürkiyeAydın Adnan Menderes University, Faculty of Veterinary Medicine, Department of Microbiology, TR-09010 Aydın - TürkiyeHarran University, Faculty of Veterinary Medicine, Department of Microbiology, TR-63290 Şanlıurfa - TürkiyeHarran University, Faculty of Veterinary Medicine, Department of Microbiology, TR-63290 Şanlıurfa - TürkiyeMiddle East Technical University, Bioinformatic Institute, TR-06800 Ankara - TürkiyeThis study aimed to investigate the presence and distribution of virulence determinants and their antimicrobial susceptibilities of 44 Pseudomonas aeruginosa isolates obtained from clinical bovine mastitis cases. In addition, selected 6 P. aeruginosa isolates were further characterized using whole-genome sequencing (WGS). Based on the presence of T3SS-related genes, 25% of the isolates were found to carry exoU and/or exoS genes belonging to invasive (exoU-/exoS+, 18.2%) and cytotoxic (exoU+/exoS-, 6.8%) strains. But, none of the isolates carried both exoU and exoS genes. In terms of other virulence genes examined, various virulence gene profiles were observed among the isolates. The majority of the isolates (72.7%) were susceptible to all tested antimicrobials. Resistance rates to ciprofloxacin and carbapenems (imipenem and meropenem) were determined as 25% and 4.5%, respectively. WGS analysis indicated the presence of different resistome, but fosA genes in all isolates, and different combinations of mutations in gyrA, parC, oprD, efflux pump, and genes playing a role in the regulation of ampC gene expression. Different sequence types (STs) and serotypes were found in representative isolates with the occurrence of the O11-ST235 clone, which is a worldwide multidrug-resistant high-risk clone representing a serious public health threat. The findings of this study provide valuable information on P. aeruginosa isolated from clinical bovine mastitis cases and current antimicrobial resistance levels and virulence determinants.https://vetdergikafkas.org/pdf.php?id=2951antimicrobial resistanceclinical bovine mastitispseudomonas aeruginosavirulencewhole-genome sequenci |
| spellingShingle | Özkan ASLANTAŞ Süheyla TÜRKYILMAZ Oktay KESKİN Ayfer GÜLLÜ YÜCETEPE Kaan BÜYÜKALTAY Molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis cases Kafkas Universitesi Veteriner Fakültesi Dergisi antimicrobial resistance clinical bovine mastitis pseudomonas aeruginosa virulence whole-genome sequenci |
| title | Molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis cases |
| title_full | Molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis cases |
| title_fullStr | Molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis cases |
| title_full_unstemmed | Molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis cases |
| title_short | Molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis cases |
| title_sort | molecular characterization of pseudomonas aeruginosa isolated from clinical bovine mastitis cases |
| topic | antimicrobial resistance clinical bovine mastitis pseudomonas aeruginosa virulence whole-genome sequenci |
| url | https://vetdergikafkas.org/pdf.php?id=2951 |
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