Far-infrared ray radiation promotes neurite outgrowth of neuron-like PC12 cells through AKT1 signaling
Background/purpose: Far-infrared (FIR) therapy is a safe and noninvasive source for medical applications. Animal study has shown the effects of FIR in promoting nerve repair. However, the cellular mechanism is not well known. Nerve growth factor (NGF) treated neuron-like PC12 cells for neurite outgr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-02-01
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| Series: | Journal of the Formosan Medical Association |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664618304595 |
| _version_ | 1811235037520068608 |
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| author | Jue-Long Wang Yong-Chong Lin Tai-Horng Young Min-Huey Chen |
| author_facet | Jue-Long Wang Yong-Chong Lin Tai-Horng Young Min-Huey Chen |
| author_sort | Jue-Long Wang |
| collection | DOAJ |
| description | Background/purpose: Far-infrared (FIR) therapy is a safe and noninvasive source for medical applications. Animal study has shown the effects of FIR in promoting nerve repair. However, the cellular mechanism is not well known. Nerve growth factor (NGF) treated neuron-like PC12 cells for neurite outgrowth have been widely employed as the in vitro model for neural regeneration. Methods: In this study, we tried to evaluate the potential of FIR in promoting neurite outgrowth and related mechanism by using NGF-treated neuron-like PC12 cells as a cellular model. We found that FIR could promote neurites outgrowth of neuron-like PC12 cells at earlier culture period. Results: The neurite outgrowth-enhancing effect of FIR irradiation was more obvious when lower NGF concentration (1 ng/ml and 10 ng/ml) was added into the medium. We also found that FIR had no thermal effects on culture medium. The effects of FIR in promoting neurite outgrowth were dose dependent, and higher power density of FIR provided more effects for improving neurite outgrowth. The mechanism of FIR in promoting neurite outgrowth was through AKT1 pathway. Conclusion: The effects of FIR irradiation on promoting neurite outgrowth and neural regeneration of NGF-treated neuron-like PC12 cells are dose dependent and through activation of AKT1 phosphorylation. This study provided important information for understanding the cellular mechanism of FIR in promoting neurite outgrowth and possible neural regeneration for further clinical applications. Keywords: Far-infrared, Neurite outgrowth, Neural regeneration, Neuron-like PC12 cells |
| first_indexed | 2024-04-12T11:45:32Z |
| format | Article |
| id | doaj.art-cdb20884d09d4df98e7e8a1bd1921f05 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-04-12T11:45:32Z |
| publishDate | 2019-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-cdb20884d09d4df98e7e8a1bd1921f052022-12-22T03:34:24ZengElsevierJournal of the Formosan Medical Association0929-66462019-02-011182600610Far-infrared ray radiation promotes neurite outgrowth of neuron-like PC12 cells through AKT1 signalingJue-Long Wang0Yong-Chong Lin1Tai-Horng Young2Min-Huey Chen3Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; School of Medicine, National Defense Medical Center, Taipei, Taiwan; Department of Nursing, Chung Hwa University of Medical Technology, Tainan, TaiwanInstitute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, TaiwanInstitute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan; Corresponding author. Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, TaiwanGraduate Institute of Clinical Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Corresponding author. Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, No. 1, Chang-de St., Taipei 100, Taiwan. Fax: +886 2 2383 1346.Background/purpose: Far-infrared (FIR) therapy is a safe and noninvasive source for medical applications. Animal study has shown the effects of FIR in promoting nerve repair. However, the cellular mechanism is not well known. Nerve growth factor (NGF) treated neuron-like PC12 cells for neurite outgrowth have been widely employed as the in vitro model for neural regeneration. Methods: In this study, we tried to evaluate the potential of FIR in promoting neurite outgrowth and related mechanism by using NGF-treated neuron-like PC12 cells as a cellular model. We found that FIR could promote neurites outgrowth of neuron-like PC12 cells at earlier culture period. Results: The neurite outgrowth-enhancing effect of FIR irradiation was more obvious when lower NGF concentration (1 ng/ml and 10 ng/ml) was added into the medium. We also found that FIR had no thermal effects on culture medium. The effects of FIR in promoting neurite outgrowth were dose dependent, and higher power density of FIR provided more effects for improving neurite outgrowth. The mechanism of FIR in promoting neurite outgrowth was through AKT1 pathway. Conclusion: The effects of FIR irradiation on promoting neurite outgrowth and neural regeneration of NGF-treated neuron-like PC12 cells are dose dependent and through activation of AKT1 phosphorylation. This study provided important information for understanding the cellular mechanism of FIR in promoting neurite outgrowth and possible neural regeneration for further clinical applications. Keywords: Far-infrared, Neurite outgrowth, Neural regeneration, Neuron-like PC12 cellshttp://www.sciencedirect.com/science/article/pii/S0929664618304595 |
| spellingShingle | Jue-Long Wang Yong-Chong Lin Tai-Horng Young Min-Huey Chen Far-infrared ray radiation promotes neurite outgrowth of neuron-like PC12 cells through AKT1 signaling Journal of the Formosan Medical Association |
| title | Far-infrared ray radiation promotes neurite outgrowth of neuron-like PC12 cells through AKT1 signaling |
| title_full | Far-infrared ray radiation promotes neurite outgrowth of neuron-like PC12 cells through AKT1 signaling |
| title_fullStr | Far-infrared ray radiation promotes neurite outgrowth of neuron-like PC12 cells through AKT1 signaling |
| title_full_unstemmed | Far-infrared ray radiation promotes neurite outgrowth of neuron-like PC12 cells through AKT1 signaling |
| title_short | Far-infrared ray radiation promotes neurite outgrowth of neuron-like PC12 cells through AKT1 signaling |
| title_sort | far infrared ray radiation promotes neurite outgrowth of neuron like pc12 cells through akt1 signaling |
| url | http://www.sciencedirect.com/science/article/pii/S0929664618304595 |
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