Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis
The integrity of plant genetic information is constantly challenged by various internal and external factors. Therefore, plants use a sophisticated molecular network to identify, signal and repair damaged DNA. Here, we report on the identification and analysis of four uncharacterized Arabidopsis BRC...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1023358/full |
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| author | Jovanka Vladejić Fen Yang Fen Yang Eva Dvořák Tomaštíková Jaroslav Doležel Jan J. Palecek Ales Pecinka Ales Pecinka |
| author_facet | Jovanka Vladejić Fen Yang Fen Yang Eva Dvořák Tomaštíková Jaroslav Doležel Jan J. Palecek Ales Pecinka Ales Pecinka |
| author_sort | Jovanka Vladejić |
| collection | DOAJ |
| description | The integrity of plant genetic information is constantly challenged by various internal and external factors. Therefore, plants use a sophisticated molecular network to identify, signal and repair damaged DNA. Here, we report on the identification and analysis of four uncharacterized Arabidopsis BRCT5 DOMAIN CONTAINING PROTEINs (BCPs). Proteins with the BRCT5 domain are frequently involved in the maintenance of genome stability across eukaryotes. The screening for sensitivity to induced DNA damage identified BCP1 as the most interesting candidate. We show that BCP1 loss of function mutants are hypersensitive to various types of DNA damage and accumulate an increased number of dead cells in root apical meristems upon DNA damage. Analysis of publicly available sog1 transcriptomic and SOG1 genome-wide DNA binding data revealed that BCP1 is inducible by gamma radiation and is a direct target of this key DNA damage signaling transcription factor. Importantly, bcp1 plants showed a reduced frequency of somatic homologous recombination in response to both endogenous and induced DNA damage. Altogether, we identified a novel plant-specific DNA repair factor that acts downstream of SOG1 in homology-based repair. |
| first_indexed | 2024-04-13T06:03:37Z |
| format | Article |
| id | doaj.art-cdb2666dcb884f2cb474dae172e7c105 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T06:03:37Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-cdb2666dcb884f2cb474dae172e7c1052022-12-22T02:59:20ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10233581023358Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in ArabidopsisJovanka Vladejić0Fen Yang1Fen Yang2Eva Dvořák Tomaštíková3Jaroslav Doležel4Jan J. Palecek5Ales Pecinka6Ales Pecinka7Institute of Experimental Botany (IEB), Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), Olomouc, CzechiaInstitute of Experimental Botany (IEB), Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), Olomouc, CzechiaDepartment of Cell Biology and Genetics, Faculty of Science, Palacký University, Olomouc, CzechiaInstitute of Experimental Botany (IEB), Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), Olomouc, CzechiaInstitute of Experimental Botany (IEB), Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), Olomouc, CzechiaNational Centre for Biomolecular Research (NCBR), Faculty of Science, Masaryk University, Brno, CzechiaInstitute of Experimental Botany (IEB), Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), Olomouc, CzechiaDepartment of Cell Biology and Genetics, Faculty of Science, Palacký University, Olomouc, CzechiaThe integrity of plant genetic information is constantly challenged by various internal and external factors. Therefore, plants use a sophisticated molecular network to identify, signal and repair damaged DNA. Here, we report on the identification and analysis of four uncharacterized Arabidopsis BRCT5 DOMAIN CONTAINING PROTEINs (BCPs). Proteins with the BRCT5 domain are frequently involved in the maintenance of genome stability across eukaryotes. The screening for sensitivity to induced DNA damage identified BCP1 as the most interesting candidate. We show that BCP1 loss of function mutants are hypersensitive to various types of DNA damage and accumulate an increased number of dead cells in root apical meristems upon DNA damage. Analysis of publicly available sog1 transcriptomic and SOG1 genome-wide DNA binding data revealed that BCP1 is inducible by gamma radiation and is a direct target of this key DNA damage signaling transcription factor. Importantly, bcp1 plants showed a reduced frequency of somatic homologous recombination in response to both endogenous and induced DNA damage. Altogether, we identified a novel plant-specific DNA repair factor that acts downstream of SOG1 in homology-based repair.https://www.frontiersin.org/articles/10.3389/fpls.2022.1023358/fullDNA damage repairgenome stabilityBRCT domainBRCT5 domainhomologous recombinationArabidopsis |
| spellingShingle | Jovanka Vladejić Fen Yang Fen Yang Eva Dvořák Tomaštíková Jaroslav Doležel Jan J. Palecek Ales Pecinka Ales Pecinka Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis Frontiers in Plant Science DNA damage repair genome stability BRCT domain BRCT5 domain homologous recombination Arabidopsis |
| title | Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis |
| title_full | Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis |
| title_fullStr | Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis |
| title_full_unstemmed | Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis |
| title_short | Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis |
| title_sort | analysis of brct5 domain containing proteins reveals a new component of dna damage repair in arabidopsis |
| topic | DNA damage repair genome stability BRCT domain BRCT5 domain homologous recombination Arabidopsis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1023358/full |
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