Highly Localized Enrichment of <i>Trypanosoma brucei</i> Parasites Using Dielectrophoresis
Human African trypanosomiasis (HAT), also known as sleeping sickness, is a vector-borne neglected tropical disease endemic to rural sub-Saharan Africa. Current methods of early detection in the affected rural communities generally begin with general screening using the card agglutination test for tr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/11/6/625 |
| _version_ | 1827714221222133760 |
|---|---|
| author | Devin Keck Callie Stuart Josie Duncan Emily Gullette Rodrigo Martinez-Duarte |
| author_facet | Devin Keck Callie Stuart Josie Duncan Emily Gullette Rodrigo Martinez-Duarte |
| author_sort | Devin Keck |
| collection | DOAJ |
| description | Human African trypanosomiasis (HAT), also known as sleeping sickness, is a vector-borne neglected tropical disease endemic to rural sub-Saharan Africa. Current methods of early detection in the affected rural communities generally begin with general screening using the card agglutination test for trypanosomiasis (CATT), a serological test. However, the gold standard for confirmation of trypanosomiasis remains the direct observation of the causative parasite, <i>Trypanosoma brucei</i>. Here, we present the use of dielectrophoresis (DEP) to enrich <i>T. brucei</i> parasites in specific locations to facilitate their identification in a future diagnostic assay. DEP refers to physical movement that can be selectively induced on the parasites when exposing them to electric field gradients of specific magnitude, phase and frequency. The long-term goal of our work is to use DEP to selectively trap and enrich <i>T. brucei</i> in specific locations while eluting all other cells in a sample. This would allow for a diagnostic test that enables the user to characterize the presence of parasites in specific locations determined <i>a priori</i> instead of relying on scanning a sample. In the work presented here, we report the characterization of the conditions that lead to high enrichment, 780% in 50 s, of the parasite in specific locations using an array of titanium microelectrodes. |
| first_indexed | 2024-03-10T18:52:42Z |
| format | Article |
| id | doaj.art-cdba393716914372b063f392cd13ae33 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T18:52:42Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-cdba393716914372b063f392cd13ae332023-11-20T05:04:28ZengMDPI AGMicromachines2072-666X2020-06-0111662510.3390/mi11060625Highly Localized Enrichment of <i>Trypanosoma brucei</i> Parasites Using DielectrophoresisDevin Keck0Callie Stuart1Josie Duncan2Emily Gullette3Rodrigo Martinez-Duarte4Multiscale Manufacturing Laboratory, Department of Mechanical Engineering, Clemson University, Clemson, SC 29634, USAMultiscale Manufacturing Laboratory, Department of Mechanical Engineering, Clemson University, Clemson, SC 29634, USAMultiscale Manufacturing Laboratory, Department of Mechanical Engineering, Clemson University, Clemson, SC 29634, USAMultiscale Manufacturing Laboratory, Department of Mechanical Engineering, Clemson University, Clemson, SC 29634, USAMultiscale Manufacturing Laboratory, Department of Mechanical Engineering, Clemson University, Clemson, SC 29634, USAHuman African trypanosomiasis (HAT), also known as sleeping sickness, is a vector-borne neglected tropical disease endemic to rural sub-Saharan Africa. Current methods of early detection in the affected rural communities generally begin with general screening using the card agglutination test for trypanosomiasis (CATT), a serological test. However, the gold standard for confirmation of trypanosomiasis remains the direct observation of the causative parasite, <i>Trypanosoma brucei</i>. Here, we present the use of dielectrophoresis (DEP) to enrich <i>T. brucei</i> parasites in specific locations to facilitate their identification in a future diagnostic assay. DEP refers to physical movement that can be selectively induced on the parasites when exposing them to electric field gradients of specific magnitude, phase and frequency. The long-term goal of our work is to use DEP to selectively trap and enrich <i>T. brucei</i> in specific locations while eluting all other cells in a sample. This would allow for a diagnostic test that enables the user to characterize the presence of parasites in specific locations determined <i>a priori</i> instead of relying on scanning a sample. In the work presented here, we report the characterization of the conditions that lead to high enrichment, 780% in 50 s, of the parasite in specific locations using an array of titanium microelectrodes.https://www.mdpi.com/2072-666X/11/6/625sleeping sicknessHuman African trypanosomiasistrypanosomatitaniumdielectrophoresis |
| spellingShingle | Devin Keck Callie Stuart Josie Duncan Emily Gullette Rodrigo Martinez-Duarte Highly Localized Enrichment of <i>Trypanosoma brucei</i> Parasites Using Dielectrophoresis Micromachines sleeping sickness Human African trypanosomiasis trypanosoma titanium dielectrophoresis |
| title | Highly Localized Enrichment of <i>Trypanosoma brucei</i> Parasites Using Dielectrophoresis |
| title_full | Highly Localized Enrichment of <i>Trypanosoma brucei</i> Parasites Using Dielectrophoresis |
| title_fullStr | Highly Localized Enrichment of <i>Trypanosoma brucei</i> Parasites Using Dielectrophoresis |
| title_full_unstemmed | Highly Localized Enrichment of <i>Trypanosoma brucei</i> Parasites Using Dielectrophoresis |
| title_short | Highly Localized Enrichment of <i>Trypanosoma brucei</i> Parasites Using Dielectrophoresis |
| title_sort | highly localized enrichment of i trypanosoma brucei i parasites using dielectrophoresis |
| topic | sleeping sickness Human African trypanosomiasis trypanosoma titanium dielectrophoresis |
| url | https://www.mdpi.com/2072-666X/11/6/625 |
| work_keys_str_mv | AT devinkeck highlylocalizedenrichmentofitrypanosomabruceiiparasitesusingdielectrophoresis AT calliestuart highlylocalizedenrichmentofitrypanosomabruceiiparasitesusingdielectrophoresis AT josieduncan highlylocalizedenrichmentofitrypanosomabruceiiparasitesusingdielectrophoresis AT emilygullette highlylocalizedenrichmentofitrypanosomabruceiiparasitesusingdielectrophoresis AT rodrigomartinezduarte highlylocalizedenrichmentofitrypanosomabruceiiparasitesusingdielectrophoresis |