Ginseng, Tribulus Extracts and Pollen Grains Supplementation Improves Sexual State, Testes Redox Status, and Testicular Histology in Nile Tilapia Males
This study aimed to investigate the effect of dietary supplementation of three natural antioxidants on sex hormone levels, enzymatic and non-enzymatic antioxidant systems, and histological changes in the testes of male Nile tilapia, <i>Oreochromis niloticus</i>. A total of 210 male Nile...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/875 |
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| author | Abdallah Tageldein Mansour Ahmed Saud Alsaqufi Eglal Ali Omar Hossam S. El-Beltagi Tarek Mohamed Srour Mokhtar Ibrahim Yousef |
| author_facet | Abdallah Tageldein Mansour Ahmed Saud Alsaqufi Eglal Ali Omar Hossam S. El-Beltagi Tarek Mohamed Srour Mokhtar Ibrahim Yousef |
| author_sort | Abdallah Tageldein Mansour |
| collection | DOAJ |
| description | This study aimed to investigate the effect of dietary supplementation of three natural antioxidants on sex hormone levels, enzymatic and non-enzymatic antioxidant systems, and histological changes in the testes of male Nile tilapia, <i>Oreochromis niloticus</i>. A total of 210 male Nile tilapia were distributed into seven treatments (three replicates for each) with an initial weight of 3.67 g fish<sup>−1</sup>. The fish were fed experimental diets (32% crude protein) without supplementation as control or supplemented with ginseng extract (GE; 0.2 and 0.4 g GE kg<sup>−1</sup> diet), <i>Tribulus terrestris</i> extract (TT; 0.6 and 1.2 g TT kg<sup>−1</sup> diet), and date palm pollen grains (DPPG; 3 and 6 g DPPG kg<sup>−1</sup> diet) for 84 days. The results revealed a significant increase in the luteinizing hormone level with TT, DPPG, and GE supplementation increased the levels by 22.9%, 18.5%, and 17.6%, respectively. The testosterone level also increased significantly with TT<sub>1.2</sub>, GE<sub>0.4</sub>, TT<sub>0.6</sub>, and DPPG<sub>6</sub> by 86.23%, 64.49%, 57.40%, and 24.62%, respectively. The antioxidant status in the testis homogenate showed a significant decrease in the level of thiobarbituric acid-reactive substances when using different dietary substances. In addition, glutathione reduced contents, glutathione <i>S</i>-transferases, glutathione peroxidase, catalase, and superoxide dismutase activities significantly increased with different dietary supplementation in a dose-dependent manner. The histological evaluation revealed normal histological features of the testes in all treatments with increasing active seminiferous tubules (%) in GE, TT, and DPPG supplemented groups, especially with the highest levels. In conclusion, the dietary supplementation of GE, TT, and DPPG enhanced sex hormones level, redox status, and testis structure and could improve the male reproductive performance of Nile tilapia. |
| first_indexed | 2024-03-10T03:26:45Z |
| format | Article |
| id | doaj.art-cdbca2750e864429a800d5e5d4ad99c3 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:26:45Z |
| publishDate | 2022-04-01 |
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| series | Antioxidants |
| spelling | doaj.art-cdbca2750e864429a800d5e5d4ad99c32023-11-23T09:51:05ZengMDPI AGAntioxidants2076-39212022-04-0111587510.3390/antiox11050875Ginseng, Tribulus Extracts and Pollen Grains Supplementation Improves Sexual State, Testes Redox Status, and Testicular Histology in Nile Tilapia MalesAbdallah Tageldein Mansour0Ahmed Saud Alsaqufi1Eglal Ali Omar2Hossam S. El-Beltagi3Tarek Mohamed Srour4Mokhtar Ibrahim Yousef5Al Bilad Bank Scholarly Chair for Food Security in Saudi Arabia, The Deanship of Scientific Research, The Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Al-Ahsa 31982, Saudi ArabiaAl Bilad Bank Scholarly Chair for Food Security in Saudi Arabia, The Deanship of Scientific Research, The Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Fish and Animal Production, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptAl Bilad Bank Scholarly Chair for Food Security in Saudi Arabia, The Deanship of Scientific Research, The Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Fish and Animal Production, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptDepartment of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria 21526, EgyptThis study aimed to investigate the effect of dietary supplementation of three natural antioxidants on sex hormone levels, enzymatic and non-enzymatic antioxidant systems, and histological changes in the testes of male Nile tilapia, <i>Oreochromis niloticus</i>. A total of 210 male Nile tilapia were distributed into seven treatments (three replicates for each) with an initial weight of 3.67 g fish<sup>−1</sup>. The fish were fed experimental diets (32% crude protein) without supplementation as control or supplemented with ginseng extract (GE; 0.2 and 0.4 g GE kg<sup>−1</sup> diet), <i>Tribulus terrestris</i> extract (TT; 0.6 and 1.2 g TT kg<sup>−1</sup> diet), and date palm pollen grains (DPPG; 3 and 6 g DPPG kg<sup>−1</sup> diet) for 84 days. The results revealed a significant increase in the luteinizing hormone level with TT, DPPG, and GE supplementation increased the levels by 22.9%, 18.5%, and 17.6%, respectively. The testosterone level also increased significantly with TT<sub>1.2</sub>, GE<sub>0.4</sub>, TT<sub>0.6</sub>, and DPPG<sub>6</sub> by 86.23%, 64.49%, 57.40%, and 24.62%, respectively. The antioxidant status in the testis homogenate showed a significant decrease in the level of thiobarbituric acid-reactive substances when using different dietary substances. In addition, glutathione reduced contents, glutathione <i>S</i>-transferases, glutathione peroxidase, catalase, and superoxide dismutase activities significantly increased with different dietary supplementation in a dose-dependent manner. The histological evaluation revealed normal histological features of the testes in all treatments with increasing active seminiferous tubules (%) in GE, TT, and DPPG supplemented groups, especially with the highest levels. In conclusion, the dietary supplementation of GE, TT, and DPPG enhanced sex hormones level, redox status, and testis structure and could improve the male reproductive performance of Nile tilapia.https://www.mdpi.com/2076-3921/11/5/875antioxidant statussexual performanceNile tilapia<i>Tribulus terrestris</i>ginsengdate palm pollen |
| spellingShingle | Abdallah Tageldein Mansour Ahmed Saud Alsaqufi Eglal Ali Omar Hossam S. El-Beltagi Tarek Mohamed Srour Mokhtar Ibrahim Yousef Ginseng, Tribulus Extracts and Pollen Grains Supplementation Improves Sexual State, Testes Redox Status, and Testicular Histology in Nile Tilapia Males Antioxidants antioxidant status sexual performance Nile tilapia <i>Tribulus terrestris</i> ginseng date palm pollen |
| title | Ginseng, Tribulus Extracts and Pollen Grains Supplementation Improves Sexual State, Testes Redox Status, and Testicular Histology in Nile Tilapia Males |
| title_full | Ginseng, Tribulus Extracts and Pollen Grains Supplementation Improves Sexual State, Testes Redox Status, and Testicular Histology in Nile Tilapia Males |
| title_fullStr | Ginseng, Tribulus Extracts and Pollen Grains Supplementation Improves Sexual State, Testes Redox Status, and Testicular Histology in Nile Tilapia Males |
| title_full_unstemmed | Ginseng, Tribulus Extracts and Pollen Grains Supplementation Improves Sexual State, Testes Redox Status, and Testicular Histology in Nile Tilapia Males |
| title_short | Ginseng, Tribulus Extracts and Pollen Grains Supplementation Improves Sexual State, Testes Redox Status, and Testicular Histology in Nile Tilapia Males |
| title_sort | ginseng tribulus extracts and pollen grains supplementation improves sexual state testes redox status and testicular histology in nile tilapia males |
| topic | antioxidant status sexual performance Nile tilapia <i>Tribulus terrestris</i> ginseng date palm pollen |
| url | https://www.mdpi.com/2076-3921/11/5/875 |
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