Kinetics of the serological response up to one year after tularemia
Serological analysis is the predominant method used to diagnose tularemia, a zoonotic disease caused by the highly virulent bacterium F. tularensis. We determined F. tularensis-specific IgM and IgG antibody titers by an LPS-based ELISA assay on five occasions one to twelve months after onset of ulce...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1072703/full |
| _version_ | 1828070418354798592 |
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| author | Helena Lindgren Johan Eklund Kjell Eneslätt Anders Sjöstedt |
| author_facet | Helena Lindgren Johan Eklund Kjell Eneslätt Anders Sjöstedt |
| author_sort | Helena Lindgren |
| collection | DOAJ |
| description | Serological analysis is the predominant method used to diagnose tularemia, a zoonotic disease caused by the highly virulent bacterium F. tularensis. We determined F. tularensis-specific IgM and IgG antibody titers by an LPS-based ELISA assay on five occasions one to twelve months after onset of ulceroglandular tularemia in 19 individuals. Peak IgM antibody titers were observed at the one-month time point and peak IgG antibody titers at the two-month time point. Both IgG and IgM antibody levels declined linearly thereafter with rather similar kinetics. Compared to the average one-month antibody titers, average IgG titers were not significantly lower before the 12-month time point and IgM titers before the 4-month time point. All, but one average titer, were significantly increased compared to the cut-off of the assay. Average IgG and IgM titers were significantly lower for the group = 69 years old compared to the group < 69 years. Collectively, the data demonstrate a persistence of F. tularensis-specific IgM and IgG antibody titers for at least 12 months after ulceroglandular tularemia. Thus, low, but significantly elevated F. tularensis-specific antibody titers are of limited diagnostic value since they are not indicative of ongoing tularemia. |
| first_indexed | 2024-04-11T00:36:21Z |
| format | Article |
| id | doaj.art-cdbd9c00de5b4ff18e561c8c437bdea7 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T00:36:21Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-cdbd9c00de5b4ff18e561c8c437bdea72023-01-06T17:44:37ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-01-011210.3389/fcimb.2022.10727031072703Kinetics of the serological response up to one year after tularemiaHelena Lindgren0Johan Eklund1Kjell Eneslätt2Anders Sjöstedt3Department of Clinical Microbiology, Umeå University, Umeå, SwedenLjusdal-Ramsjö Primary Care Centre, Ljusdal, SwedenDepartment of Clinical Microbiology, Umeå University, Umeå, SwedenDepartment of Clinical Microbiology, Umeå University, Umeå, SwedenSerological analysis is the predominant method used to diagnose tularemia, a zoonotic disease caused by the highly virulent bacterium F. tularensis. We determined F. tularensis-specific IgM and IgG antibody titers by an LPS-based ELISA assay on five occasions one to twelve months after onset of ulceroglandular tularemia in 19 individuals. Peak IgM antibody titers were observed at the one-month time point and peak IgG antibody titers at the two-month time point. Both IgG and IgM antibody levels declined linearly thereafter with rather similar kinetics. Compared to the average one-month antibody titers, average IgG titers were not significantly lower before the 12-month time point and IgM titers before the 4-month time point. All, but one average titer, were significantly increased compared to the cut-off of the assay. Average IgG and IgM titers were significantly lower for the group = 69 years old compared to the group < 69 years. Collectively, the data demonstrate a persistence of F. tularensis-specific IgM and IgG antibody titers for at least 12 months after ulceroglandular tularemia. Thus, low, but significantly elevated F. tularensis-specific antibody titers are of limited diagnostic value since they are not indicative of ongoing tularemia.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1072703/fulltularemiaserological responsekineticsone yearelderly |
| spellingShingle | Helena Lindgren Johan Eklund Kjell Eneslätt Anders Sjöstedt Kinetics of the serological response up to one year after tularemia Frontiers in Cellular and Infection Microbiology tularemia serological response kinetics one year elderly |
| title | Kinetics of the serological response up to one year after tularemia |
| title_full | Kinetics of the serological response up to one year after tularemia |
| title_fullStr | Kinetics of the serological response up to one year after tularemia |
| title_full_unstemmed | Kinetics of the serological response up to one year after tularemia |
| title_short | Kinetics of the serological response up to one year after tularemia |
| title_sort | kinetics of the serological response up to one year after tularemia |
| topic | tularemia serological response kinetics one year elderly |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1072703/full |
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