Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production by <i>Lactobacillus plantarum</i> J23
At present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production by <i>Lac...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/2079-7737/9/7/171 |
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| author | Jianming Zhang Yushan Bu Chengcheng Zhang Huaxi Yi Daqun Liu Jingkai Jiao |
| author_facet | Jianming Zhang Yushan Bu Chengcheng Zhang Huaxi Yi Daqun Liu Jingkai Jiao |
| author_sort | Jianming Zhang |
| collection | DOAJ |
| description | At present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production by <i>Lactobacillus plantarum</i> J23 was developed. First, the effects of the composition of MRS broth on bacteriocin Lac-B23 production and bacterial growth were researched by a one variable at a time approach. Then, a Plackett-Burman design was used to screen significant components for production. Finally, the steepest ascent and central composite designs were used to obtain an optimum medium. The final composition of the modified MRS was much simpler than MRS broth, and the modified MRS contained only glucose, yeast extract, dipotassium phosphate, manganese sulfate monohydrate, Tween 80 and sodium acetate anhydrous. The highest bacteriocin Lac-B23 production reached 2560 activity units (AU)/mL in the modified MRS, which is nine times higher than that in MRS broth (280 AU/mL). Meanwhile, the cost per liter of the modified MRS (8.56 Ren Min Bi (RMB)/L) is 34.70% the cost of MRS broth (13.11 RMB/L), and the cost per arbitrary units of bacteriocin Lac-B23 in the modified MRS is approximately fourteen times more convenient (3.34 RMB/10<sup>6</sup> AU) than in the MRS broth (46.82 RMB/10<sup>6</sup> AU). |
| first_indexed | 2024-03-10T18:26:08Z |
| format | Article |
| id | doaj.art-cdbebd0eb57c4848b79ae929d4a71861 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T18:26:08Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-cdbebd0eb57c4848b79ae929d4a718612023-11-20T07:03:03ZengMDPI AGBiology2079-77372020-07-019717110.3390/biology9070171Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production by <i>Lactobacillus plantarum</i> J23Jianming Zhang0Yushan Bu1Chengcheng Zhang2Huaxi Yi3Daqun Liu4Jingkai Jiao5Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310016, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266100, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310016, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266100, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310016, ChinaState Key Laboratory of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, ChinaAt present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production by <i>Lactobacillus plantarum</i> J23 was developed. First, the effects of the composition of MRS broth on bacteriocin Lac-B23 production and bacterial growth were researched by a one variable at a time approach. Then, a Plackett-Burman design was used to screen significant components for production. Finally, the steepest ascent and central composite designs were used to obtain an optimum medium. The final composition of the modified MRS was much simpler than MRS broth, and the modified MRS contained only glucose, yeast extract, dipotassium phosphate, manganese sulfate monohydrate, Tween 80 and sodium acetate anhydrous. The highest bacteriocin Lac-B23 production reached 2560 activity units (AU)/mL in the modified MRS, which is nine times higher than that in MRS broth (280 AU/mL). Meanwhile, the cost per liter of the modified MRS (8.56 Ren Min Bi (RMB)/L) is 34.70% the cost of MRS broth (13.11 RMB/L), and the cost per arbitrary units of bacteriocin Lac-B23 in the modified MRS is approximately fourteen times more convenient (3.34 RMB/10<sup>6</sup> AU) than in the MRS broth (46.82 RMB/10<sup>6</sup> AU).https://www.mdpi.com/2079-7737/9/7/171bacteriocin Lac-B23low-costhigh-efficiencyculture medium |
| spellingShingle | Jianming Zhang Yushan Bu Chengcheng Zhang Huaxi Yi Daqun Liu Jingkai Jiao Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production by <i>Lactobacillus plantarum</i> J23 Biology bacteriocin Lac-B23 low-cost high-efficiency culture medium |
| title | Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production by <i>Lactobacillus plantarum</i> J23 |
| title_full | Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production by <i>Lactobacillus plantarum</i> J23 |
| title_fullStr | Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production by <i>Lactobacillus plantarum</i> J23 |
| title_full_unstemmed | Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production by <i>Lactobacillus plantarum</i> J23 |
| title_short | Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production by <i>Lactobacillus plantarum</i> J23 |
| title_sort | development of a low cost and high efficiency culture medium for bacteriocin lac b23 production by i lactobacillus plantarum i j23 |
| topic | bacteriocin Lac-B23 low-cost high-efficiency culture medium |
| url | https://www.mdpi.com/2079-7737/9/7/171 |
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