Watershed-Induced Limnological and Microbial Status in Two Oligotrophic Andean Lakes Exposed to the Same Climatic Scenario
Laguna Negra and Lo Encañado are two oligotrophic Andean lakes forming part of the system fed by meltwater from distinct glacial tongues of the Echaurren glacier in central Chile, which is in a recession period. The recent increase in temperature and decline in precipitation have led to an increase...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00357/full |
| _version_ | 1818522705567154176 |
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| author | Alex Echeverría-Vega Guillermo Chong Antonio E. Serrano Mariela Guajardo Olga Encalada Victor Parro Yolanda Blanco Luis Rivas Kevin C. Rose Mercedes Moreno-Paz José A. Luque José A. Luque Nathalie A. Cabrol Nathalie A. Cabrol Cecilia S. Demergasso |
| author_facet | Alex Echeverría-Vega Guillermo Chong Antonio E. Serrano Mariela Guajardo Olga Encalada Victor Parro Yolanda Blanco Luis Rivas Kevin C. Rose Mercedes Moreno-Paz José A. Luque José A. Luque Nathalie A. Cabrol Nathalie A. Cabrol Cecilia S. Demergasso |
| author_sort | Alex Echeverría-Vega |
| collection | DOAJ |
| description | Laguna Negra and Lo Encañado are two oligotrophic Andean lakes forming part of the system fed by meltwater from distinct glacial tongues of the Echaurren glacier in central Chile, which is in a recession period. The recent increase in temperature and decline in precipitation have led to an increase of glacial meltwater and sediments entering these lakes. Although the lacustrine systems are also hydrogeologically connected, the limnology of the lakes is strongly controlled by the surface processes related to the respective sub-watersheds and hydrology. Watershed characteristics (area and length, slope, lithology, resistance to erosion, among others) affect the chemical and physical characteristics of both lakes (e.g., nutrient concentration and turbidity). We studied physical and chemical variables and performed 16S rRNA amplicon sequencing to determine the specific microbial signature of the lakes. The transparency, temperature, turbidity and concentrations of chlorophyll-a, dissolved organic matter, nutrients and the total number of cells, revealed the different status of both lakes at the time of sampling. The predominant bacterial groups in both lakes were Proteobacteria, Verrucomicrobia, and Bacteroidetes. Interestingly, the contribution of phototrophs was significantly higher in LN compared to LE (13 and 4% respectively) and the major fraction corresponded to Anoxygenic Phototrophs (AP) represented by Chloroflexi, Alpha, and Betaproteobacteria. Multivariate analyses showed that the nutrient levels and the light availability of both lakes, which finally depend on the hydrological characteristics of the respective watersheds, explain the differential community composition/function. The abundance of a diverse photoheterotrophic bacterioplankton community suggests that the ability to utilize solar energy along with organic and inorganic substrates is a key function in these oligotrophic mountain lakes. |
| first_indexed | 2024-12-11T05:36:37Z |
| format | Article |
| id | doaj.art-cdc23534ad8d4b79a1bef4d570c75b3c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T05:36:37Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cdc23534ad8d4b79a1bef4d570c75b3c2022-12-22T01:19:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-03-01910.3389/fmicb.2018.00357296225Watershed-Induced Limnological and Microbial Status in Two Oligotrophic Andean Lakes Exposed to the Same Climatic ScenarioAlex Echeverría-Vega0Guillermo Chong1Antonio E. Serrano2Mariela Guajardo3Olga Encalada4Victor Parro5Yolanda Blanco6Luis Rivas7Kevin C. Rose8Mercedes Moreno-Paz9José A. Luque10José A. Luque11Nathalie A. Cabrol12Nathalie A. Cabrol13Cecilia S. Demergasso14Centro de Biotecnología, Universidad Católica del Norte, Antofagasta, ChileDepartamento de Ciencias Geológicas, Universidad Católica de Norte, Antofagasta, ChileCentro de Biotecnología, Universidad Católica del Norte, Antofagasta, ChileCentro de Investigación Científica y Tecnológica para la Minería, Antofagasta, ChileCentro de Biotecnología, Universidad Católica del Norte, Antofagasta, ChileDepartment of Molecular Evolution, Centro de Astrobiología (CSIC-INTA), Madrid, SpainDepartment of Molecular Evolution, Centro de Astrobiología (CSIC-INTA), Madrid, SpainDepartment of Molecular Evolution, Centro de Astrobiología (CSIC-INTA), Madrid, SpainDepartment of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, United StatesDepartment of Molecular Evolution, Centro de Astrobiología (CSIC-INTA), Madrid, SpainDepartamento de Ciencias Geológicas, Universidad Católica de Norte, Antofagasta, ChileCentro de Investigación Tecnológica del Agua en el Desierto (CEITSAZA), Universidad Católica del Norte, Antofagasta, ChileCarl Sagan Center, SETI Institute, Mountain View, CA, United StatesSpace Science Division, NASA Ames Research Center, Moffett Field, CA, United StatesCentro de Biotecnología, Universidad Católica del Norte, Antofagasta, ChileLaguna Negra and Lo Encañado are two oligotrophic Andean lakes forming part of the system fed by meltwater from distinct glacial tongues of the Echaurren glacier in central Chile, which is in a recession period. The recent increase in temperature and decline in precipitation have led to an increase of glacial meltwater and sediments entering these lakes. Although the lacustrine systems are also hydrogeologically connected, the limnology of the lakes is strongly controlled by the surface processes related to the respective sub-watersheds and hydrology. Watershed characteristics (area and length, slope, lithology, resistance to erosion, among others) affect the chemical and physical characteristics of both lakes (e.g., nutrient concentration and turbidity). We studied physical and chemical variables and performed 16S rRNA amplicon sequencing to determine the specific microbial signature of the lakes. The transparency, temperature, turbidity and concentrations of chlorophyll-a, dissolved organic matter, nutrients and the total number of cells, revealed the different status of both lakes at the time of sampling. The predominant bacterial groups in both lakes were Proteobacteria, Verrucomicrobia, and Bacteroidetes. Interestingly, the contribution of phototrophs was significantly higher in LN compared to LE (13 and 4% respectively) and the major fraction corresponded to Anoxygenic Phototrophs (AP) represented by Chloroflexi, Alpha, and Betaproteobacteria. Multivariate analyses showed that the nutrient levels and the light availability of both lakes, which finally depend on the hydrological characteristics of the respective watersheds, explain the differential community composition/function. The abundance of a diverse photoheterotrophic bacterioplankton community suggests that the ability to utilize solar energy along with organic and inorganic substrates is a key function in these oligotrophic mountain lakes.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00357/fulloligotrophic lakesmicrobial communitiesglacial melting16srRNA gene sequencingwatershed influence |
| spellingShingle | Alex Echeverría-Vega Guillermo Chong Antonio E. Serrano Mariela Guajardo Olga Encalada Victor Parro Yolanda Blanco Luis Rivas Kevin C. Rose Mercedes Moreno-Paz José A. Luque José A. Luque Nathalie A. Cabrol Nathalie A. Cabrol Cecilia S. Demergasso Watershed-Induced Limnological and Microbial Status in Two Oligotrophic Andean Lakes Exposed to the Same Climatic Scenario Frontiers in Microbiology oligotrophic lakes microbial communities glacial melting 16srRNA gene sequencing watershed influence |
| title | Watershed-Induced Limnological and Microbial Status in Two Oligotrophic Andean Lakes Exposed to the Same Climatic Scenario |
| title_full | Watershed-Induced Limnological and Microbial Status in Two Oligotrophic Andean Lakes Exposed to the Same Climatic Scenario |
| title_fullStr | Watershed-Induced Limnological and Microbial Status in Two Oligotrophic Andean Lakes Exposed to the Same Climatic Scenario |
| title_full_unstemmed | Watershed-Induced Limnological and Microbial Status in Two Oligotrophic Andean Lakes Exposed to the Same Climatic Scenario |
| title_short | Watershed-Induced Limnological and Microbial Status in Two Oligotrophic Andean Lakes Exposed to the Same Climatic Scenario |
| title_sort | watershed induced limnological and microbial status in two oligotrophic andean lakes exposed to the same climatic scenario |
| topic | oligotrophic lakes microbial communities glacial melting 16srRNA gene sequencing watershed influence |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00357/full |
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