Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro
A new chemical component, cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide, was isolated from Angelica sinensis and its structure elucidated from its NMR and MS spectra and confirmed by X-ray single crystal diffraction analysis. We also explored the antioxidative properties of...
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| Format: | Article |
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MDPI AG
2013-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/1/520 |
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| author | Xuedong Liu Cuiping Yan Zhipeng Chen Baochang Cai Ding Zhao Fangzhou Yin Zebin Weng Guangming Yang Weidong Li Dan Liu Yang Pan Yu Wu |
| author_facet | Xuedong Liu Cuiping Yan Zhipeng Chen Baochang Cai Ding Zhao Fangzhou Yin Zebin Weng Guangming Yang Weidong Li Dan Liu Yang Pan Yu Wu |
| author_sort | Xuedong Liu |
| collection | DOAJ |
| description | A new chemical component, cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide, was isolated from Angelica sinensis and its structure elucidated from its NMR and MS spectra and confirmed by X-ray single crystal diffraction analysis. We also explored the antioxidative properties of cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide on human umbilical vein endothelial cells (HUVECs) against injuries induced by hydrogen peroxide (H2O2) using an MTT assay and flow cytometry analysis. In addition, the activities of superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH), nitric oxide (NO) and reactive oxygen species (ROS) were determined. We found that cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide increased the viability of HUVECs injured by H2O2 in a dose-dependent manner, reduced the apoptosis of HUVEC, and enhanced HUVEC proliferation. Our results demonstrated the remarkable in vitro antioxidative activities of this compound, indicating that it could be a potential antioxidant with protective effects against H2O2-induced HUVEC injuries. |
| first_indexed | 2024-12-11T04:51:42Z |
| format | Article |
| id | doaj.art-cdc3ec6b257a4363a8ae75ddfe3cbaa4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T04:51:42Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cdc3ec6b257a4363a8ae75ddfe3cbaa42022-12-22T01:20:23ZengMDPI AGMolecules1420-30492013-01-0118152053410.3390/molecules18010520Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in VitroXuedong LiuCuiping YanZhipeng ChenBaochang CaiDing ZhaoFangzhou YinZebin WengGuangming YangWeidong LiDan LiuYang PanYu WuA new chemical component, cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide, was isolated from Angelica sinensis and its structure elucidated from its NMR and MS spectra and confirmed by X-ray single crystal diffraction analysis. We also explored the antioxidative properties of cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide on human umbilical vein endothelial cells (HUVECs) against injuries induced by hydrogen peroxide (H2O2) using an MTT assay and flow cytometry analysis. In addition, the activities of superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH), nitric oxide (NO) and reactive oxygen species (ROS) were determined. We found that cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide increased the viability of HUVECs injured by H2O2 in a dose-dependent manner, reduced the apoptosis of HUVEC, and enhanced HUVEC proliferation. Our results demonstrated the remarkable in vitro antioxidative activities of this compound, indicating that it could be a potential antioxidant with protective effects against H2O2-induced HUVEC injuries.http://www.mdpi.com/1420-3049/18/1/520cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilideantioxidanthuman umbilical vein endothelial cellcell cycle |
| spellingShingle | Xuedong Liu Cuiping Yan Zhipeng Chen Baochang Cai Ding Zhao Fangzhou Yin Zebin Weng Guangming Yang Weidong Li Dan Liu Yang Pan Yu Wu Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro Molecules cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide antioxidant human umbilical vein endothelial cell cell cycle |
| title | Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro |
| title_full | Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro |
| title_fullStr | Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro |
| title_full_unstemmed | Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro |
| title_short | Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro |
| title_sort | antioxidant properties of cis z z 039 3a 7a 039 7a 3a 039 dihydroxy ligustilide on human umbilical vein endothelial cells in vitro |
| topic | cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide antioxidant human umbilical vein endothelial cell cell cycle |
| url | http://www.mdpi.com/1420-3049/18/1/520 |
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