Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon Gonads
Sturgeons are highly valuable species from both a conservation and economic point of view. Their very sought-after caviar extracted from mature females has both a rich culture around it and opens the way for interesting research. Because of the advanced age at which females and males could be identi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1011 |
| _version_ | 1827288335880552448 |
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| author | Alexandru Burcea Iulia Elena Florescu (Gune) Gina-Oana Pistol Marilena Maereanu Andreea Dudu Marieta Costache Sergiu Emil Georgescu |
| author_facet | Alexandru Burcea Iulia Elena Florescu (Gune) Gina-Oana Pistol Marilena Maereanu Andreea Dudu Marieta Costache Sergiu Emil Georgescu |
| author_sort | Alexandru Burcea |
| collection | DOAJ |
| description | Sturgeons are highly valuable species from both a conservation and economic point of view. Their very sought-after caviar extracted from mature females has both a rich culture around it and opens the way for interesting research. Because of the advanced age at which females and males could be identified in aquaculture it is important to develop a set of molecular markers that could make the distinction easy. For this, a better understanding of the sexual development of sturgeons is necessary. We selected the Dmrt1 (doublesex and mab-3 related transcription factor 1) and the Cyp17a1 (17α-hydroxylase) markers with the scope of investigating the gonad development of sturgeon individuals. Both markers appear to be expressed in the gonad but no statistically significant difference in expression is observed between females and males. This could mean that the markers are equally involved in female and male sexual development at the stage of sampling. This study paves the way for a better understanding of sturgeon sexual development. |
| first_indexed | 2024-04-24T11:30:19Z |
| format | Article |
| id | doaj.art-cdc4a6313bbc4122adb7e8865e551889 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:30:19Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-cdc4a6313bbc4122adb7e8865e5518892024-04-10T10:54:05ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-0151178781011Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon GonadsAlexandru Burcea0Iulia Elena Florescu (Gune)1Gina-Oana Pistol2Marilena Maereanu3Andreea Dudu4Marieta Costache5Sergiu Emil Georgescu6University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, zip code 050095, Bucharest, Splaiul Independentei 91-95, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, zip code 050095, Bucharest, Splaiul Independentei 91-95, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, zip code 050095, Bucharest, Splaiul Independentei 91-95, RomaniaS.C. Danube Research-Consulting S.R.L., zip code 825200, Isaccea, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, zip code 050095, Bucharest, Splaiul Independentei 91-95, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, zip code 050095, Bucharest, Splaiul Independentei 91-95, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, zip code 050095, Bucharest, Splaiul Independentei 91-95, RomaniaSturgeons are highly valuable species from both a conservation and economic point of view. Their very sought-after caviar extracted from mature females has both a rich culture around it and opens the way for interesting research. Because of the advanced age at which females and males could be identified in aquaculture it is important to develop a set of molecular markers that could make the distinction easy. For this, a better understanding of the sexual development of sturgeons is necessary. We selected the Dmrt1 (doublesex and mab-3 related transcription factor 1) and the Cyp17a1 (17α-hydroxylase) markers with the scope of investigating the gonad development of sturgeon individuals. Both markers appear to be expressed in the gonad but no statistically significant difference in expression is observed between females and males. This could mean that the markers are equally involved in female and male sexual development at the stage of sampling. This study paves the way for a better understanding of sturgeon sexual development.https://spasb.ro/index.php/public_html/article/view/1011gonad developmentwestern blotsturgeonsaquaculture |
| spellingShingle | Alexandru Burcea Iulia Elena Florescu (Gune) Gina-Oana Pistol Marilena Maereanu Andreea Dudu Marieta Costache Sergiu Emil Georgescu Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon Gonads Scientific Papers Animal Science and Biotechnologies gonad development western blot sturgeons aquaculture |
| title | Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon Gonads |
| title_full | Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon Gonads |
| title_fullStr | Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon Gonads |
| title_full_unstemmed | Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon Gonads |
| title_short | Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon Gonads |
| title_sort | dmrt1 and cyp17a1 protein detection and relative quantification in best beluga hybrid sturgeon gonads |
| topic | gonad development western blot sturgeons aquaculture |
| url | https://spasb.ro/index.php/public_html/article/view/1011 |
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