Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor
Abstract Background The etiology of prostate cancer (PCa) is multiple and complex. Among the causes recently cited are chronic infections engendered by microorganisms that often go unnoticed. A typical illustration of such a case is infection due to mollicutes bacteria. Generally known by their lurk...
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | Infectious Agents and Cancer |
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| Online Access: | https://doi.org/10.1186/s13027-021-00344-9 |
| _version_ | 1818456556042190848 |
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| author | Osama Mohammed Saed Abdul-Wahab Mishari H. Al-Shyarba Boutheina Ben Abdelmoumen Mardassi Nessrine Sassi Majed Saad Shaya Al Fayi Hassan Otifi Abdullah Hassan Al Murea Béhija Mlik Elhem Yacoub |
| author_facet | Osama Mohammed Saed Abdul-Wahab Mishari H. Al-Shyarba Boutheina Ben Abdelmoumen Mardassi Nessrine Sassi Majed Saad Shaya Al Fayi Hassan Otifi Abdullah Hassan Al Murea Béhija Mlik Elhem Yacoub |
| author_sort | Osama Mohammed Saed Abdul-Wahab |
| collection | DOAJ |
| description | Abstract Background The etiology of prostate cancer (PCa) is multiple and complex. Among the causes recently cited are chronic infections engendered by microorganisms that often go unnoticed. A typical illustration of such a case is infection due to mollicutes bacteria. Generally known by their lurking nature, urogenital mollicutes are the most incriminated in PCa. This study was thus carried out in an attempt to establish the presence of these mollicutes by PCR in biopsies of confirmed PCa patients and to evaluate their prevalence. Methods A total of 105 Formalin-Fixed Paraffin-Embedded prostate tissues collected from 50 patients suffering from PCa and 55 with benign prostate hyperplasia were subjected to PCR amplification targeting species-specific genes of 5 urogenital mollicutes species, Mycoplasma genitalium, M. hominis, M. fermentans, Ureaplasma parvum, and U. urealyticum. PCR products were then sequenced to confirm species identification. Results significance was statistically assessed using Chi-square and Odds ratio tests. Results PCR amplification showed no positive results for M. genitalium, M. hominis, and M. fermentans in all tested patients. Strikingly, Ureaplasma spp. were detected among 30% (15/50) of PCa patients. Nucleotide sequencing further confirmed the identified ureaplasma species, which were distributed as follows: 7 individuals with only U. parvum, 5 with only U. urealyticum, and 3 co-infection cases. Association of the two ureaplasma species with PCa cases proved statistically significant (P < 0.05), and found to represent a risk factor. Of note, Ureaplasma spp. were mostly identified in patients aged 60 and above with prostatic specific antigen (PSA) level > 4 ng/ml and an invasive malignant prostate tumor (Gleason score 8–10). Conclusions This study uncovered a significant association of Ureaplasma spp. with PCa arguing in favour of their potential involvement in this condition. Yet, this finding, though statistically supported, warrants a thorough investigation at a much larger scale. |
| first_indexed | 2024-12-14T22:28:32Z |
| format | Article |
| id | doaj.art-cdc562406916471a892fb752f2acc830 |
| institution | Directory Open Access Journal |
| issn | 1750-9378 |
| language | English |
| last_indexed | 2024-12-14T22:28:32Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Agents and Cancer |
| spelling | doaj.art-cdc562406916471a892fb752f2acc8302022-12-21T22:45:17ZengBMCInfectious Agents and Cancer1750-93782021-01-0116111110.1186/s13027-021-00344-9Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumorOsama Mohammed Saed Abdul-Wahab0Mishari H. Al-Shyarba1Boutheina Ben Abdelmoumen Mardassi2Nessrine Sassi3Majed Saad Shaya Al Fayi4Hassan Otifi5Abdullah Hassan Al Murea6Béhija Mlik7Elhem Yacoub8Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, King Khalid UniversityDepartment of Surgery, College of Medicine, King Khalid UniversitySpecialized Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El-ManarSpecialized Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El-ManarDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, King Khalid UniversityDepartment of Pathology, College of Medicine, King Khalid UniversityLaboratory Department, Aseer Central HospitalSpecialized Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El-ManarSpecialized Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El-ManarAbstract Background The etiology of prostate cancer (PCa) is multiple and complex. Among the causes recently cited are chronic infections engendered by microorganisms that often go unnoticed. A typical illustration of such a case is infection due to mollicutes bacteria. Generally known by their lurking nature, urogenital mollicutes are the most incriminated in PCa. This study was thus carried out in an attempt to establish the presence of these mollicutes by PCR in biopsies of confirmed PCa patients and to evaluate their prevalence. Methods A total of 105 Formalin-Fixed Paraffin-Embedded prostate tissues collected from 50 patients suffering from PCa and 55 with benign prostate hyperplasia were subjected to PCR amplification targeting species-specific genes of 5 urogenital mollicutes species, Mycoplasma genitalium, M. hominis, M. fermentans, Ureaplasma parvum, and U. urealyticum. PCR products were then sequenced to confirm species identification. Results significance was statistically assessed using Chi-square and Odds ratio tests. Results PCR amplification showed no positive results for M. genitalium, M. hominis, and M. fermentans in all tested patients. Strikingly, Ureaplasma spp. were detected among 30% (15/50) of PCa patients. Nucleotide sequencing further confirmed the identified ureaplasma species, which were distributed as follows: 7 individuals with only U. parvum, 5 with only U. urealyticum, and 3 co-infection cases. Association of the two ureaplasma species with PCa cases proved statistically significant (P < 0.05), and found to represent a risk factor. Of note, Ureaplasma spp. were mostly identified in patients aged 60 and above with prostatic specific antigen (PSA) level > 4 ng/ml and an invasive malignant prostate tumor (Gleason score 8–10). Conclusions This study uncovered a significant association of Ureaplasma spp. with PCa arguing in favour of their potential involvement in this condition. Yet, this finding, though statistically supported, warrants a thorough investigation at a much larger scale.https://doi.org/10.1186/s13027-021-00344-9Prostate cancerUrogenital mollicutesPCRSequencing |
| spellingShingle | Osama Mohammed Saed Abdul-Wahab Mishari H. Al-Shyarba Boutheina Ben Abdelmoumen Mardassi Nessrine Sassi Majed Saad Shaya Al Fayi Hassan Otifi Abdullah Hassan Al Murea Béhija Mlik Elhem Yacoub Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor Infectious Agents and Cancer Prostate cancer Urogenital mollicutes PCR Sequencing |
| title | Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor |
| title_full | Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor |
| title_fullStr | Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor |
| title_full_unstemmed | Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor |
| title_short | Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor |
| title_sort | molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor |
| topic | Prostate cancer Urogenital mollicutes PCR Sequencing |
| url | https://doi.org/10.1186/s13027-021-00344-9 |
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